脾脏在大鼠急性胰腺炎炎症介质释放中的作用

鲁正; 朱言亮; 何长林; 刘牧林; 刘金新

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脾脏在大鼠急性胰腺炎炎症介质释放中的作用

鲁正 , 朱言亮 , 何长林 , 刘牧林 , 刘金新

文章导航 > 蚌埠医学院学报 > 2005, 30 > 2: (110-112)

引用本文:

Citation:

脾脏在大鼠急性胰腺炎炎症介质释放中的作用

1. 蚌埠医学院附属医院普外科, 安徽蚌埠 233004(现为上海第二医科大学外科学博士研究生);
2. 华中科技大学同济医学院, 湖北武汉 430030

作者简介: 鲁正(1972-),男,安徽亳州人,博士研究生,主治医师,讲师.

基金项目:
安徽省教育厅自然科学研究资助项目(99jl0148)
中图分类号: R657.51;R331.21

Experimental study on the influence of spleen on the release of inflammatory mediator in rats with acute pancreatitis

1. Department of General Surgery, Affiliated Hospital of Bengbu Medical College, Bengbu 233004;
2. Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, China
CLC number: R657.51;R331.21

摘要: 目的: 探讨脾脏在全身炎症反应综合征(SIRS)中对炎症介质释放的作用。方法: 大鼠胆胰管内注入3%牛磺胆酸钠(0.7ml/kg)及胰蛋白酶(3000u/kg)制成急性胰腺炎模型以引起SIRS。实验大鼠随机分成4组:假手术组,脾切除组,脾切除+急性胰腺炎组,急性胰腺炎组。分别观察各组血清淀粉酶、TNF-α、IL-1β、IL-6及IL-10水平,取末段回肠行光镜及透射电镜观察肠黏膜受损情况。结果: 脾切除+急性胰腺炎组TNF-α、IL-1β及IL-10均低于急性胰腺炎组(P<0.01);病理学检查显示,脾切除+急性胰腺炎组肠黏膜上皮仅轻微水肿,肠黏膜屏障基本完整,而急性胰腺炎组肠黏膜上皮水肿明显,绒毛坏死,上皮细胞变性,炎性细胞浸润。结论: 脾脏在急性炎症反应早期,可以明显促进炎症介质的产生和释放,加重炎症反应。脾脏切除后可减少促炎因子的产生和释放,肠黏膜屏障受损减轻。
- 胰腺炎 /
- 脾/生理学 /
- 炎症介质 /
- 大鼠
Abstract: Objective: To investigate the influence of spleen on the production and release of inflammatory mediator in acute pancreatitis.Methods: Acute necrotizing pancreatitis(ANP) was induced in SD rats by injecting 3% sodium taurocholate and trypsin into the biliopancreatic duct.All rats were divided into four groups randomly:sham operation group,splenectomy group,acute pareatitis with splenectomy group,and acute pancreatitis group.The serum levels of TNF-α,IL-1β,IL-6 and IL-10 in each group were examined.The terminal ileum was excised to observe the injury of intestinal mucosal barrier by optical microscope and electronic microscope.Results: The serum levels of TNF-α,IL-1β and IL-10 in the acute pareatitis with splenectomy group were significantly lower than those in the acute pancreatitis group(P<0.01).Histological examination revealed mild edema of mucosal epithelium and normally appearing intact mucosal barrier in the acute pareatitis with splenectomy group,whereas acute pancreatitis group revealed severe injuries including apparent edema,necrosis of the intestinal villi,degeneration of epithelial cell and infiltration of neutrophils.Conclusions: Spleen may promote the production and release of inflammatory mediators in acute inflammatory response,and aggravate the inflammatory response.Splenectomy can reduce the production and release of inflammatory mediators,thus decrease the damage of intestinal mucosal barrier.
- pancreatitis /
- spleen/physiology /
- inflammatory mediator /
- rats

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脾脏在大鼠急性胰腺炎炎症介质释放中的作用

作者简介: 鲁正(1972-),男,安徽亳州人,博士研究生,主治医师,讲师.

1. 蚌埠医学院附属医院普外科, 安徽蚌埠 233004(现为上海第二医科大学外科学博士研究生);
2. 华中科技大学同济医学院, 湖北武汉 430030

收稿日期: 2004-09-08

基金项目: 安徽省教育厅自然科学研究资助项目(99jl0148)

关键词:

摘要: 目的: 探讨脾脏在全身炎症反应综合征(SIRS)中对炎症介质释放的作用。方法: 大鼠胆胰管内注入3%牛磺胆酸钠(0.7ml/kg)及胰蛋白酶(3000u/kg)制成急性胰腺炎模型以引起SIRS。实验大鼠随机分成4组:假手术组,脾切除组,脾切除+急性胰腺炎组,急性胰腺炎组。分别观察各组血清淀粉酶、TNF-α、IL-1β、IL-6及IL-10水平,取末段回肠行光镜及透射电镜观察肠黏膜受损情况。结果: 脾切除+急性胰腺炎组TNF-α、IL-1β及IL-10均低于急性胰腺炎组(P<0.01);病理学检查显示,脾切除+急性胰腺炎组肠黏膜上皮仅轻微水肿,肠黏膜屏障基本完整,而急性胰腺炎组肠黏膜上皮水肿明显,绒毛坏死,上皮细胞变性,炎性细胞浸润。结论: 脾脏在急性炎症反应早期,可以明显促进炎症介质的产生和释放,加重炎症反应。脾脏切除后可减少促炎因子的产生和释放,肠黏膜屏障受损减轻。

English Abstract

Experimental study on the influence of spleen on the release of inflammatory mediator in rats with acute pancreatitis

1. Department of General Surgery, Affiliated Hospital of Bengbu Medical College, Bengbu 233004;
2. Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, China

Received Date: 2004-09-08

Abstract: Objective: To investigate the influence of spleen on the production and release of inflammatory mediator in acute pancreatitis.Methods: Acute necrotizing pancreatitis(ANP) was induced in SD rats by injecting 3% sodium taurocholate and trypsin into the biliopancreatic duct.All rats were divided into four groups randomly:sham operation group,splenectomy group,acute pareatitis with splenectomy group,and acute pancreatitis group.The serum levels of TNF-α,IL-1β,IL-6 and IL-10 in each group were examined.The terminal ileum was excised to observe the injury of intestinal mucosal barrier by optical microscope and electronic microscope.Results: The serum levels of TNF-α,IL-1β and IL-10 in the acute pareatitis with splenectomy group were significantly lower than those in the acute pancreatitis group(P<0.01).Histological examination revealed mild edema of mucosal epithelium and normally appearing intact mucosal barrier in the acute pareatitis with splenectomy group,whereas acute pancreatitis group revealed severe injuries including apparent edema,necrosis of the intestinal villi,degeneration of epithelial cell and infiltration of neutrophils.Conclusions: Spleen may promote the production and release of inflammatory mediators in acute inflammatory response,and aggravate the inflammatory response.Splenectomy can reduce the production and release of inflammatory mediators,thus decrease the damage of intestinal mucosal barrier.