Abstract: Objective: To investigate the growth characteristics of Cordyceps militaris hyphae.Methods: In the Cordyceps militaris strains' growth test in culture medium,the growth rate of hyphae and the amount of conidiophores were studied.Results: The data proved that the different Cordyceps militaris strains' growth rate of hayphae and the amount of conidiophores were similar when cultivated in the same culture medium,meanwhile,the results showed obvious differences when the same strain grew in different culture media.Conclusions: The components of culture medium may make great effects on the Cordyceps militaris hyphae's growth rate and the amount of conidiophores.