抗肿瘤坏死因子-α单克隆抗体对阻塞性黄疸大鼠肝脏保护作用的实验研究

邱兆磊; 王振杰; 刘牧林; 黄建康; 董慧明; 郑士友

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

抗肿瘤坏死因子-α单克隆抗体对阻塞性黄疸大鼠肝脏保护作用的实验研究

邱兆磊 , 王振杰 , 刘牧林 , 黄建康 , 董慧明 , 郑士友

文章导航 > 蚌埠医学院学报 > 2006, 31 > 3: (235-237)

引用本文:

Citation:

抗肿瘤坏死因子-α单克隆抗体对阻塞性黄疸大鼠肝脏保护作用的实验研究

1. 蚌埠医学院附属医院急诊外科, 安徽蚌埠 233004;
2. 蚌埠医学院附属医院胃肠外科, 安徽蚌埠 233004;
3. 蚌埠医学院附属医院肿瘤外科, 安徽蚌埠 233004

作者简介: 邱兆磊(1976-),男,硕士研究生.

基金项目:
安徽省教育厅自然科学研究资助项目(2002kj219)
中图分类号: R575.63;R442.4

Experimental study about the protective effect of anti-TNF-α McAb on liver of rats with obstructive jaundice

1. Department of Emergency Surgery, Affiliated Hospital of Bengbu Medical College, Bengbu 233004, China;
2. Department of Gastrointestinal Surgery, Affiliated Hospital of Bengbu Medical College, Bengbu 233004, China;
3. Department of Surgical Oncology, Affiliated Hospital of Bengbu Medical College, Bengbu 233004, China
CLC number: R575.63;R442.4

摘要: 目的: 探讨肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)在阻塞性黄疸大鼠肝损伤中的作用及抗肿瘤坏死因子-α单克隆抗体(anti-TNF-αMcAb)的保护作用。方法: 将雄性SD大鼠24只随机分为胆总管结扎组(BDL)、BDL+anti-TNF-αMcAb组、假手术组(SO)。BDL+anti-TNF-αMcAb组治疗于胆总管结扎后第3天开始经尾静脉注射anti-TNF-αMcAb (1 mg/kg),连用5天;BDL组和SO组以同样的方法注射等量的生理盐水。分别检测各组大鼠血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)和TNF-α水平,并在光镜和电镜下观察各组大鼠肝组织的病理变化。结果: BDL组及BDL+anti-TNF-αMcAb治疗组大鼠血清ALT、AST和TNF-α水平均高于SO组(P<0.01),但BDL+anti-TNF-αMcAb治疗组增高的程度均低于BDL组(P<0.05~P<0.01),并且肝组织病理改变也较轻。SO组肝组织无明显病理改变。结论: TNF-α可能是阻塞性黄疸时肝损伤的重要介导因子,使用抗肿瘤坏死因子-α单克隆抗体,可以减轻阻塞性黄疸时肝损伤。
- 胆管阻塞,肝外 /
- 黄疸 /
- 肿瘤坏死因子-α /
- 抗肿瘤坏死因子-α单克隆抗体 /
- 肝损伤 /
- 大鼠
Abstract: Objective: To investigate the role of tumor necrosis factor-α(TNF-α) in liver damage in rats with obstructive jaundice and the protective effect of anti-TNF-αMcAb.Methods: Twenty-four male Sprague-Dawley rats were randomly divided into three groups:bile duct-ligation(BDL) group,bile duct-ligation+anti-TNF-αMcAb(BDL+anti-TNF-αMcAb) group and sham-operation(SO) group.BDL+anti-TNF-αMcAb group was subjected to damage with intravenous administration of anti-TNF-αMcAb(1mg/kg) for five days,starting from the 3rd day after bile duct-ligation;BDL group and SO group were administered with saline in the same manner.The levels of serum alanine amiotransferase(ALT),aspartate aminotransferase(AST) and TNF-α were detected in each group.The histopathology was observed by light and electron microscopy in the liver of rats with obstructive jaundice.Results: The levels of serum ALT,AST and TNF-α in BDL group and BDL+anti-TNF-αMcAb group were markedly higher than those in SO group(P<0.01),and the levels of serum ALT,AST and TNF-α in BDL+anti-TNF-αMcAb group were much lower than those in BDL group(P<0.05 to P<0.01),the changes of liver histopathology were minor in BDL+anti-TNF-αMcAb group.SO group had no apparent change.Conclusions: TNF-α may be one of the most important mediators in live damage induced by obstructive jaundice,and the use of anti-TNF-αMcAb can relieve the liver damage.
- bile duct obstruction /
- jaundice /
- tumor necrosis factor-α /
- anti-TNF-α monoclonal antibody /
- liver damage /
- rats

[1]	徐艳秋 , 王洪巨 . 慢性应激大鼠血清肿瘤坏死因子-α、白细胞介素-6的变化及其对心率和血压的影响. 蚌埠医学院学报, 2011, 36(2): 127-129.
[2]	徐志鹏 , 陈硬 , 宋琦 , 李启梁 , 朱恒 , 邱兆磊 , 纪忠 , 王振杰 . 醋酸钠林格液联合乌司他丁对失血性休克大鼠肝组织NF-κB p65蛋白表达及其细胞因子的影响. 蚌埠医学院学报, 2018, 43(10): 1334-1338. doi: 10.13898/j.cnki.issn.1000-2200.2018.10.016
[3]	温雪萍 , 王忠慧 , 丁梅芬 , 李艳 . 慢性阻塞性肺疾病患者血清中白细胞介素-10、肿瘤坏死因子-α、转化生长因子-β的变化. 蚌埠医学院学报, 2010, 35(12): 1272-1274.
[4]	马良龙 , 刘瑞林 , 刘牧林 . 急性胰腺炎大鼠胰腺组织核因子-κB动态变化. 蚌埠医学院学报, 2005, 30(2): 107-109.
[5]	郑列祥 , 杨邦明 , 杨军 , 陈志萍 . CT导引下经皮穿刺肝内胆管胆汁外引流术治疗恶性阻塞性黄疸28例. 蚌埠医学院学报, 2012, 36(8): 974-975.
[6]	张长春 , 邵晨 , 许刚 , 朱坤 , 叶雨辰 , 程飞 . 肿瘤坏死因子-α作用下椎间盘退变动物模型的建立. 蚌埠医学院学报, 2018, 43(10): 1329-1333,1338. doi: 10.13898/j.cnki.issn.1000-2200.2018.10.015
[7]	朱玉乾 , 郑传明 , 宁长青 . 腹腔穿刺引流对重症急性胰腺炎大鼠相关肝损伤的保护作用. 蚌埠医学院学报, 2023, 48(10): 1329-1332. doi: 10.13898/j.cnki.issn.1000-2200.2023.10.001
[8]	周汝娟 , 何龙锦 , 杨峰 . 血清白介素-6和肿瘤坏死因子-α与脑出血早期再出血的关系. 蚌埠医学院学报, 2010, 35(11): 1133-1134.
[9]	吴颖 , 崔朝勃 , 亢宏山 , 赵媛媛 , 刘淑红 . 乌司他丁对脓毒症患者血清肿瘤坏死因子-α、白细胞介素10、肌钙蛋白I和C反应蛋白水平的影响. 蚌埠医学院学报, 2016, 41(12): 1635-1638. doi: 10.13898/j.cnki.issn.1000-2200.2016.12.031
[10]	徐志庆 , 夏惠 , 李柏青 . 不同刺激剂对健康人和结核患者外周血T细胞产生γ干扰素和肿瘤坏死因子-α的影响. 蚌埠医学院学报, 2016, 41(3): 288-291. doi: 10.13898/j.cnki.issn.1000-2200.2016.03.003
[11]	仇大鹏 , 朱勋兵 , 李徽徽 , 袁伶俐 , 陈笑天 . 肿瘤坏死因子-α和白细胞介素-1β在脊髓型颈椎病中的表达及相关性研究. 蚌埠医学院学报, 2018, 43(7): 841-844. doi: 10.13898/j.cnki.issn.1000-2200.2018.07.001
[12]	马铭 , 袁耀宗 , 刘建生 , 田怡 , 张晓红 , 刘海玲 . 重组人生长激素联合支链氨基酸对肝硬化患者肿瘤坏死因子-α和白细胞介素-10的影响. 蚌埠医学院学报, 2015, 40(3): 357-358,361. doi: 10.13898/j.cnki.issn.1000-2200.2015.03.020
[13]	黄显元 , 游荔 . 2型糖尿病合并冠心病病人血清超敏C反应蛋白、肿瘤坏死因子-α、高迁移率蛋白B1水平变化及临床意义. 蚌埠医学院学报, 2020, 45(1): 48-50, 56. doi: 10.13898/j.cnki.issn.1000-2200.2020.01.012
[14]	袁晓宇 , 袁菊萍 , 黄中伟 . 血必净对热休克蛋白90在脓毒症小鼠肝肺损伤中表达的影响. 蚌埠医学院学报, 2016, 41(6): 717-721. doi: 10.13898/j.cnki.issn.1000-2200.2016.06.004
[15]	陆德锟 , 黄奕江 , 齐晓林 , 黄峻松 , 邵先安 , 徐长江 , 马宏昊 , 郝建 . 血必净对水下爆炸致兔急性闭合性肝损伤早期干预的效果研究. 蚌埠医学院学报, 2019, 44(2): 150-153. doi: 10.13898/j.cnki.issn.1000-2200.2019.02.003
[16]	陈晓 , 王启之 , 梁冰 , 杨庆宇 , 李琰 , 于东红 . Toll样受体4、肿瘤坏死因子-在溃疡性结肠炎大鼠结肠组织中的表达及意义. 蚌埠医学院学报, 2013, 37(2): 134-136.
[17]	朱锡玲 , 张翠玲 , 刘浩 , 余美玲 . 金匮护肝汤对四氯化碳引起小鼠急性肝损伤的保护作用. 蚌埠医学院学报, 2013, 37(3): 262-263,267.
[18]	孙硕 , 戎李 , 莫辰 , 张冰 , 高琴 , 王洪巨 , 康品方 , 张恒 . 糖尿病大鼠心肌损伤中程序性坏死通路相关蛋白的表达. 蚌埠医学院学报, 2020, 45(4): 437-441, 446. doi: 10.13898/j.cnki.issn.1000-2200.2020.04.005
[19]	李辉 , 康品方 , 徐庆梅 , 戎李 , 孙硕 , 张冰 , 高琴 , 高大胜 , 王洪巨 . 厄贝沙坦对糖尿病大鼠心肌损伤中MAPKs信号通路及相关因子的影响. 蚌埠医学院学报, 2020, 45(11): 1453-1457. doi: 10.13898/j.cnki.issn.1000-2200.2020.11.001
[20]	路黎娟 , 徐梅佳 , 董焱 . 重组人粒细胞集落刺激因子对缺血缺氧性脑损伤新生大鼠凋亡诱导因子表达的影响. 蚌埠医学院学报, 2016, 41(12): 1545-1549. doi: 10.13898/j.cnki.issn.1000-2200.2016.12.002

点击查看大图

计量

文章访问数: 3399
HTML全文浏览量: 329
PDF下载量: 3
被引次数: 0

抗肿瘤坏死因子-α单克隆抗体对阻塞性黄疸大鼠肝脏保护作用的实验研究

作者简介: 邱兆磊(1976-),男,硕士研究生.

1. 蚌埠医学院附属医院急诊外科, 安徽蚌埠 233004;
2. 蚌埠医学院附属医院胃肠外科, 安徽蚌埠 233004;
3. 蚌埠医学院附属医院肿瘤外科, 安徽蚌埠 233004

收稿日期: 2005-10-24

基金项目: 安徽省教育厅自然科学研究资助项目(2002kj219)

关键词:

摘要: 目的: 探讨肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)在阻塞性黄疸大鼠肝损伤中的作用及抗肿瘤坏死因子-α单克隆抗体(anti-TNF-αMcAb)的保护作用。方法: 将雄性SD大鼠24只随机分为胆总管结扎组(BDL)、BDL+anti-TNF-αMcAb组、假手术组(SO)。BDL+anti-TNF-αMcAb组治疗于胆总管结扎后第3天开始经尾静脉注射anti-TNF-αMcAb (1 mg/kg),连用5天;BDL组和SO组以同样的方法注射等量的生理盐水。分别检测各组大鼠血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)和TNF-α水平,并在光镜和电镜下观察各组大鼠肝组织的病理变化。结果: BDL组及BDL+anti-TNF-αMcAb治疗组大鼠血清ALT、AST和TNF-α水平均高于SO组(P<0.01),但BDL+anti-TNF-αMcAb治疗组增高的程度均低于BDL组(P<0.05~P<0.01),并且肝组织病理改变也较轻。SO组肝组织无明显病理改变。结论: TNF-α可能是阻塞性黄疸时肝损伤的重要介导因子,使用抗肿瘤坏死因子-α单克隆抗体,可以减轻阻塞性黄疸时肝损伤。

English Abstract

Experimental study about the protective effect of anti-TNF-α McAb on liver of rats with obstructive jaundice

1. Department of Emergency Surgery, Affiliated Hospital of Bengbu Medical College, Bengbu 233004, China;
2. Department of Gastrointestinal Surgery, Affiliated Hospital of Bengbu Medical College, Bengbu 233004, China;
3. Department of Surgical Oncology, Affiliated Hospital of Bengbu Medical College, Bengbu 233004, China

Received Date: 2005-10-24

Abstract: Objective: To investigate the role of tumor necrosis factor-α(TNF-α) in liver damage in rats with obstructive jaundice and the protective effect of anti-TNF-αMcAb.Methods: Twenty-four male Sprague-Dawley rats were randomly divided into three groups:bile duct-ligation(BDL) group,bile duct-ligation+anti-TNF-αMcAb(BDL+anti-TNF-αMcAb) group and sham-operation(SO) group.BDL+anti-TNF-αMcAb group was subjected to damage with intravenous administration of anti-TNF-αMcAb(1mg/kg) for five days,starting from the 3rd day after bile duct-ligation;BDL group and SO group were administered with saline in the same manner.The levels of serum alanine amiotransferase(ALT),aspartate aminotransferase(AST) and TNF-α were detected in each group.The histopathology was observed by light and electron microscopy in the liver of rats with obstructive jaundice.Results: The levels of serum ALT,AST and TNF-α in BDL group and BDL+anti-TNF-αMcAb group were markedly higher than those in SO group(P<0.01),and the levels of serum ALT,AST and TNF-α in BDL+anti-TNF-αMcAb group were much lower than those in BDL group(P<0.05 to P<0.01),the changes of liver histopathology were minor in BDL+anti-TNF-αMcAb group.SO group had no apparent change.Conclusions: TNF-α may be one of the most important mediators in live damage induced by obstructive jaundice,and the use of anti-TNF-αMcAb can relieve the liver damage.