MHC-Ⅱ、MHC-Ⅰ及-SMA在成年比格犬食管肌层的表达

周家梅; 梁亮; 涂丽莉; 朱秀玲; 张铭; 徐胜春

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MHC-Ⅱ、MHC-Ⅰ及-SMA在成年比格犬食管肌层的表达

周家梅 , 梁亮 , 涂丽莉 , 朱秀玲 , 张铭 , 徐胜春

文章导航 > 蚌埠医学院学报 > 2014, 38 > 5: (568-570)

引用本文:

Citation:

MHC-Ⅱ、MHC-Ⅰ及-SMA在成年比格犬食管肌层的表达

1.
1. 安徽中医药大学人体解剖学教研室，安徽合肥 230038；;
2.
2. 首都医科大学人体解剖学教研室，北京 100069；;
3.
3. 安徽医科大学人体解剖学教研室，安徽合肥 230038；;
4.
4. 皖南医学院人体解剖教研室，安徽芜湖 241002

作者简介: 周家梅(1980-)，女，硕士，助理实验师.

通讯作者: 徐胜春, chun028@163.com

基金项目:
安徽省自然科学基金资助项目(070413258x)

Expression of MHC-Ⅱ,MHC-Ⅰand -SMA in muscular layer of esophagus in adult dog

1.
1. Department of Anatomy，Anhui University of Chinese Medicine,Hefei Anhui 230038;
2.
2. Department of Anatomy，Capital Medical University,Beijing 100069;
3.
3. Department of Anatomy,Anhui Medical University,Hefei Anhui 230032;
4.
4. Department of Anatomy，Wannan Medical College,Wuhu Anhui 241002，China

Corresponding author: XU Shengchun, chun028@163.com

Fund Project: The natural science foundation of Anhui province (070413258x)

摘要: 目的：探讨健康成年比格犬食管肌层骨骼肌肌球蛋白重链-Ⅱ(MHC-Ⅱ)、骨骼肌肌球蛋白重链-Ⅰ(MHC-Ⅰ)和-平滑肌肌动蛋白(-SMA)的表达及空间分布。方法：采用组织学、免疫组织化学等方法对6只健康成年比格犬食管肌层中MHC-Ⅱ、MHC-Ⅰ和-SMA的表达进行观察。结果：成年比格犬食管肌层主要表达MHC-Ⅱ、MHC-Ⅰ；从食管的头端至尾端，MHC-Ⅱ的表达强度呈渐进性减弱，至食管下段近贲门部肌层仍有较强的表达；MHC-Ⅰ在食管肌层的表达由上往下逐渐加强，至食管下段近贲门部肌层有较强表达；-SMA在成年比格犬食管肌层中段下份开始有少量表达，表达由上往下逐渐加强，至食管下端，-SMA的表达由内肌层扩展至外肌层。结论：与人类不同，成年比格犬食管肌层主要由骨骼肌构成。成年比格犬食管肌层全长均表达MHC-Ⅱ和MHC-Ⅰ；-SMA主要表达在食管肌层下段。
- 肌球蛋白 /
- 肌动蛋白 /
- 比格犬 /
- 食管肌层 /
- 空间分布
Abstract: Objective:To investigate the expressions and spatial distributions of myosin heavy chain-Ⅱ(MHC-Ⅱ),myosin heavy chain-Ⅰ(MHC-Ⅰ) and -smooth muscle actin(-SMA) in muscular layer of esophagus in the healthy adult dogs.Methods:The expressions of MHC-Ⅱ,MHC-Ⅰ and -SMA in muscular layer of esophagus in 6 healthy adult dogs were detected by histology and immunohistochemistry.Results:The expressions of MHC-Ⅱ and MHC-Ⅰ in muscular layer of whole esophagus in adult dogs were detected.The expression intensity of MHC-Ⅱ gradually decreased from the head to end of esophagus,which was still strong in cardiac muscle layer around the lower esophagus.The expression intensity of MHC-Ⅰ gradually increased from the head to end of esophagus,which was still strong in cardiac muscle layer around the lower esophagus.The little -SMA expression began in the muscle layer of the middle-lower esophagus,which gradually increased from up to down of esophagus and extended from the inner muscular layer to outer muscular layer of esophagus.Conclusions:Unlike humans,the esophagus muscular layer of adult dog is mainly made up of skeletal muscle.The muscular layer of the whole esophagus in dog expresses the MHC-Ⅱ and MHC-Ⅰ,the expression of a-SMA mainly locates the muscular layer of lower esophagus.
- myosin /
- actin /
- adult dog /
- the muscular layer of esophagus /
- spatial distribution

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MHC-Ⅱ、MHC-Ⅰ及-SMA在成年比格犬食管肌层的表达

通讯作者: 徐胜春, chun028@163.com

作者简介: 周家梅(1980-)，女，硕士，助理实验师.

1. 1. 安徽中医药大学人体解剖学教研室，安徽合肥 230038；;
2. 2. 首都医科大学人体解剖学教研室，北京 100069；;
3. 3. 安徽医科大学人体解剖学教研室，安徽合肥 230038；;
4. 4. 皖南医学院人体解剖教研室，安徽芜湖 241002

收稿日期: 2013-08-04
网络出版日期: 2014-05-15

基金项目: 安徽省自然科学基金资助项目(070413258x)

关键词:

摘要: 目的：探讨健康成年比格犬食管肌层骨骼肌肌球蛋白重链-Ⅱ(MHC-Ⅱ)、骨骼肌肌球蛋白重链-Ⅰ(MHC-Ⅰ)和-平滑肌肌动蛋白(-SMA)的表达及空间分布。方法：采用组织学、免疫组织化学等方法对6只健康成年比格犬食管肌层中MHC-Ⅱ、MHC-Ⅰ和-SMA的表达进行观察。结果：成年比格犬食管肌层主要表达MHC-Ⅱ、MHC-Ⅰ；从食管的头端至尾端，MHC-Ⅱ的表达强度呈渐进性减弱，至食管下段近贲门部肌层仍有较强的表达；MHC-Ⅰ在食管肌层的表达由上往下逐渐加强，至食管下段近贲门部肌层有较强表达；-SMA在成年比格犬食管肌层中段下份开始有少量表达，表达由上往下逐渐加强，至食管下端，-SMA的表达由内肌层扩展至外肌层。结论：与人类不同，成年比格犬食管肌层主要由骨骼肌构成。成年比格犬食管肌层全长均表达MHC-Ⅱ和MHC-Ⅰ；-SMA主要表达在食管肌层下段。

English Abstract

Expression of MHC-Ⅱ,MHC-Ⅰand -SMA in muscular layer of esophagus in adult dog

Corresponding author: XU Shengchun, chun028@163.com

1. 1. Department of Anatomy，Anhui University of Chinese Medicine,Hefei Anhui 230038;
2. 2. Department of Anatomy，Capital Medical University,Beijing 100069;
3. 3. Department of Anatomy,Anhui Medical University,Hefei Anhui 230032;
4. 4. Department of Anatomy，Wannan Medical College,Wuhu Anhui 241002，China

Received Date: 2013-08-04
Available Online: 2014-05-15

Fund Project: The natural science foundation of Anhui province (070413258x)

Abstract: Objective:To investigate the expressions and spatial distributions of myosin heavy chain-Ⅱ(MHC-Ⅱ),myosin heavy chain-Ⅰ(MHC-Ⅰ) and -smooth muscle actin(-SMA) in muscular layer of esophagus in the healthy adult dogs.Methods:The expressions of MHC-Ⅱ,MHC-Ⅰ and -SMA in muscular layer of esophagus in 6 healthy adult dogs were detected by histology and immunohistochemistry.Results:The expressions of MHC-Ⅱ and MHC-Ⅰ in muscular layer of whole esophagus in adult dogs were detected.The expression intensity of MHC-Ⅱ gradually decreased from the head to end of esophagus,which was still strong in cardiac muscle layer around the lower esophagus.The expression intensity of MHC-Ⅰ gradually increased from the head to end of esophagus,which was still strong in cardiac muscle layer around the lower esophagus.The little -SMA expression began in the muscle layer of the middle-lower esophagus,which gradually increased from up to down of esophagus and extended from the inner muscular layer to outer muscular layer of esophagus.Conclusions:Unlike humans,the esophagus muscular layer of adult dog is mainly made up of skeletal muscle.The muscular layer of the whole esophagus in dog expresses the MHC-Ⅱ and MHC-Ⅰ,the expression of a-SMA mainly locates the muscular layer of lower esophagus.