不同结核杆菌抗原刺激外周血T细胞亚群产生干扰素的比较

盛玲玲; 夏惠; 孙悝; 陈勇; 李柏青

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不同结核杆菌抗原刺激外周血T细胞亚群产生干扰素的比较

盛玲玲 , 夏惠 , 孙悝 , 陈勇 , 李柏青

文章导航 > 蚌埠医学院学报 > 2014, 38 > 4: (426-428,432)

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不同结核杆菌抗原刺激外周血T细胞亚群产生干扰素的比较

1.
蚌埠医学院免疫学教研室, 感染与免疫安徽省重点实验室, 安徽蚌埠 233030

作者简介: 盛玲玲(1985-)，女，硕士研究生.

通讯作者: 李柏青, baiqingli@bbmc.edu.cn

基金项目:
国家自然科学基金面上资助项目(81072426)

Comparison of IFN- production in human peripheral blood T cell subpopulations stimulated by different antigens from Mycobacterium tuberculosis

1.
Department of Immunology, Bengbu Medical College, Anhui Key Laboratory of Infection and Immunity, Bengbu Anhui 233030, China

Corresponding author: LI Bai-qing, baiqingli@bbmc.edu.cn

摘要: 目的：比较磷酸化抗原(HDMAPP)、结核杆菌耐热抗原(Mtb-HAg)和结核杆菌低分子耐热抗原Mtb-HAg(10k)刺激外周血T细胞产生-干扰素(IFN-) 的反应能力方面的特点及差异。方法：采集正常人外周血，分别用HDMAPP、Mtb-HAg和Mtb-HAg(10k)刺激培养14h,再加入莫能霉素继续培养6 h，收集细胞，加入荧光标记单抗进行表面分子和胞内染色，然后在流式细胞仪上检测各实验组中CD3+细胞和CD3+细胞胞内IFN-的表达。结果：正常人各抗原实验组CD3+细胞和CD3+ 细胞胞内产生IFN-+细胞比率较阴性组对照组升高(P0.01)。各抗原刺激组CD3+ 细胞产生IFN-+细胞比率明显高于CD3+细胞(P0.05~P0.01)。3种抗原刺激组间CD3+细胞和CD3+细胞无明显不同(P0.05)。结论：HDMAPP、Mtb-HAg和Mtb-HAg(10k)均可以刺激外周血中T细胞中和细胞群产生IFN-，均可特异性诱导刺激CD3+细胞产生IFN-，HDMAPP、Mtb-HAg(10k)对CD3+细胞产生IFN-的表达量高于Mtb-HAg。
- 结核杆菌 /
- 抗原 /
- 干扰素 /
- T细胞 /
- T细胞
Abstract: Objective:To compare the characteristics and differences of peripheral blood T cells stimulated by HDMAPP,Mycobacterium tuberculosis heat-resistant antigen(Mtb-HAg) and low molecular peptide antigen of Mycobacterium tuberculosis(Mtb)(10 k) to produce interferon(IFN-).Methods:The peripheral blood cells from healthy adults were cultured with HDMAPP and Mtb-HAg and Mtb-HAg(10k) for 14 hours,then added Monensin to cultivate for another 6 hours.Cells were collected,and the surface molecule and intracellular cytokines were stained with different fluorochrome-conjugated mAbs.The expressions of IFN- in CD3+ and CD3+ cells of different experiment group were detected by flow cytometry.Results:The ratios of CD3+ and CD3+ cells to produce IFN- in all antigen experiment groups were higher than that in control group(P0.01).The ratios of CD3+ cells to produce IFN- in all antigen experiment groups were significantly higher than that of CD3+ cells(P0.05 to P0.01).The differences of CD3+ cells and CD3+ cells in three kinds of antigenic stimulation groups were not statistical significance(P0.05).Conclusions:CD3+ cells and CD3+ cells can produce IFN- by stimulating the peripheral blood T cells with HDMAPP,Mtb-HAg and Mtb-HAg(10k).CD3+ cells stimulated by three kinds of antigens can produce IFN-.The IFN- level in CD3+ cells stimulated by HDMAPP and Mtb-HAg(10 k) is higher than that of Mtb-HAg stimulating.
- Mycobacterium tuberculosis /
- antigen /
- interferon /
- T cell /
- T cell

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不同结核杆菌抗原刺激外周血T细胞亚群产生干扰素的比较

通讯作者: 李柏青, baiqingli@bbmc.edu.cn

作者简介: 盛玲玲(1985-)，女，硕士研究生.

1. 蚌埠医学院免疫学教研室, 感染与免疫安徽省重点实验室, 安徽蚌埠 233030

收稿日期: 2012-12-19
网络出版日期: 2014-04-15

基金项目: 国家自然科学基金面上资助项目(81072426)

关键词:

摘要: 目的：比较磷酸化抗原(HDMAPP)、结核杆菌耐热抗原(Mtb-HAg)和结核杆菌低分子耐热抗原Mtb-HAg(10k)刺激外周血T细胞产生-干扰素(IFN-) 的反应能力方面的特点及差异。方法：采集正常人外周血，分别用HDMAPP、Mtb-HAg和Mtb-HAg(10k)刺激培养14h,再加入莫能霉素继续培养6 h，收集细胞，加入荧光标记单抗进行表面分子和胞内染色，然后在流式细胞仪上检测各实验组中CD3+细胞和CD3+细胞胞内IFN-的表达。结果：正常人各抗原实验组CD3+细胞和CD3+ 细胞胞内产生IFN-+细胞比率较阴性组对照组升高(P0.01)。各抗原刺激组CD3+ 细胞产生IFN-+细胞比率明显高于CD3+细胞(P0.05~P0.01)。3种抗原刺激组间CD3+细胞和CD3+细胞无明显不同(P0.05)。结论：HDMAPP、Mtb-HAg和Mtb-HAg(10k)均可以刺激外周血中T细胞中和细胞群产生IFN-，均可特异性诱导刺激CD3+细胞产生IFN-，HDMAPP、Mtb-HAg(10k)对CD3+细胞产生IFN-的表达量高于Mtb-HAg。

English Abstract

Comparison of IFN- production in human peripheral blood T cell subpopulations stimulated by different antigens from Mycobacterium tuberculosis

Corresponding author: LI Bai-qing, baiqingli@bbmc.edu.cn

1. Department of Immunology, Bengbu Medical College, Anhui Key Laboratory of Infection and Immunity, Bengbu Anhui 233030, China

Received Date: 2012-12-19
Available Online: 2014-04-15

Abstract: Objective:To compare the characteristics and differences of peripheral blood T cells stimulated by HDMAPP,Mycobacterium tuberculosis heat-resistant antigen(Mtb-HAg) and low molecular peptide antigen of Mycobacterium tuberculosis(Mtb)(10 k) to produce interferon(IFN-).Methods:The peripheral blood cells from healthy adults were cultured with HDMAPP and Mtb-HAg and Mtb-HAg(10k) for 14 hours,then added Monensin to cultivate for another 6 hours.Cells were collected,and the surface molecule and intracellular cytokines were stained with different fluorochrome-conjugated mAbs.The expressions of IFN- in CD3+ and CD3+ cells of different experiment group were detected by flow cytometry.Results:The ratios of CD3+ and CD3+ cells to produce IFN- in all antigen experiment groups were higher than that in control group(P0.01).The ratios of CD3+ cells to produce IFN- in all antigen experiment groups were significantly higher than that of CD3+ cells(P0.05 to P0.01).The differences of CD3+ cells and CD3+ cells in three kinds of antigenic stimulation groups were not statistical significance(P0.05).Conclusions:CD3+ cells and CD3+ cells can produce IFN- by stimulating the peripheral blood T cells with HDMAPP,Mtb-HAg and Mtb-HAg(10k).CD3+ cells stimulated by three kinds of antigens can produce IFN-.The IFN- level in CD3+ cells stimulated by HDMAPP and Mtb-HAg(10 k) is higher than that of Mtb-HAg stimulating.