Ezrin蛋白与E-cadherin在食管鳞癌中的联合检测及其意义
The combining detection of Ezrin protein and E-cadherin in esophageal squamous cell carcinoma and its significance
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摘要: 目的:探讨食管鳞状细胞癌(ESCC)组织中Ezrin蛋白与E-cadherin的表达及其与肿瘤侵袭和转移的关系。方法:以30例癌旁正常黏膜组织作对照,应用免疫组织化学SP法检测76例ESCC组织标本中Ezrin蛋白与E-cadherin的表达。结果:ESCC组织中Ezrin蛋白与E-cadherin的异常表达率分别为78.95%和59.21%;而癌旁正常黏膜组织中异常表达率分别为26.67%和6.67%,两项指标差异均有统计学意义(P0.01)。Ezrin蛋白异常表达在ESCC淋巴结转移和组织分化程度间差异有统计学意义(P0.05和P0.01),E-cadherin 异常表达在ESCC组织的浸润深度、ESCC细胞的分化程度和淋巴结转移间差异均有统计学意义(P0.05~P0.01)。Ezrin蛋白与E-cadherin在ESCC中的异常表达呈正相关关系(P0.05)。E-cadherin异常表达组Ezrin蛋白表达阳性率显著高于E-cadherin正常表达患者(P0.05)。两者同时异常表达的40例中淋巴结转移高达70%,而两者同时正常表达的11例均无淋巴结转移,差异有统计学意义(P0.01)。结论:Ezrin蛋白与E-cadherin在ESCC组织中均异常表达,且呈正相关。联合检测Ezrin蛋白和E-cadherin的表达有助于综合判断ESCC的浸润、转移及预后。
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关键词:
- 食管肿瘤 /
- 肿瘤转移 /
- Ezrin蛋白 /
- E-cadherin /
- 免疫组织化学
Abstract: Objective:To investigate the expressions of Ezrin protein and E-cadherin in esophageal squamous cell carcinoma(ESCC) and their correlations with the invasion and metastasis of tumor.Methods:The expressions of Ezrin protein and E-cad in 76 ESCC tissue specimens and 30 normal tissue specimens were detected by SP method.Results:The abnormal expression rates of Ezrin protein and E-cadherin in ESCC tissue and normal tissue were 78.95% 59.21% and 26.67% 6.67%,respectively,the differences of two indicators were statistical significance(P0.01).The differences between the abnormal expression of Ezrin protein and the lymph node metastasis and tissue differentiation degree of esophageal squamous carcinoma were statistical significance(P0.05 and P0.01),the differences between the abnormal expression of E-cadherin and the invasion depth,lymph node metastasis and tissue differentiation degree of esophageal squamous carcinoma were statistical significance(P0.05 to P0.01).There was a positive correlation between the abnormal expression of Ezrin protein and E-cadherin in ESCC(P0.05).The positive expression rate of Ezrin protein in tissue specimens with abnormal E-cadherin expression was significantly higher than that in tissue specimens with normal E-cadherin expression(P0.05).The lymph node metastasis rate in 40 tissue specimens with abnormal E-cadherin and Ezrin protein expressions was 70%,no lymph node metastasis in 11 tissue specimens with normal E-cadherin and Ezrin protein expressions was found,the difference of which was statistical significance(P0.01).Conclusions:The abnormal expressions of Ezrin protein and E-cadherin in ESCC are positively correlated relationship.The combining detection of Ezrin protein and E-cadherin can help to judge the metastasis and prognosis of ESCC.-
Key words:
- esophageal neoplasms /
- neoplasm metastasis /
- Ezrin protein /
- E-cadherin /
- immunohistochemistry
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