糖皮质激素对肥胖大鼠和普通大鼠下丘脑神经肽Y mRNA的影响

范艳萍; 石建华; 项平; 胡小磊; 苗艳君; 黄詠齐

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糖皮质激素对肥胖大鼠和普通大鼠下丘脑神经肽Y mRNA的影响

范艳萍 , 石建华 , 项平 , 胡小磊 , 苗艳君 , 黄詠齐

文章导航 > 蚌埠医学院学报 > 2009, 34 > 5: (369-371)

引用本文:

Citation:

糖皮质激素对肥胖大鼠和普通大鼠下丘脑神经肽Y mRNA的影响

1. 蚌埠医学院第一附属医院内分泌科, 安徽蚌埠 233004;
2. 蚌埠医学院第一附属医院中心实验室, 安徽蚌埠 233004

作者简介: 范艳萍(1980-).女.硕士,研究生

基金项目:
国家自然科学基金资助项目(No.30440026)
中图分类号: R589.2;Q577

Influence of glucocorticoids on neuropeptide Y mRNA expression in hypothalamus of obese and nonobese rats

1. Department of Endocrinotogy, The First Affiliated Hospital of Bengbu Medical College, Bengbu Anhui 233004, China;
2. Department of Central Laboratories, The First Affiliated Hospital of Bengbu Medical College, Bengbu Anhui 233004, China
CLC number: R589.2;Q577

摘要: 目的:研究糖皮质激素对肥胖大鼠和普通大鼠下丘脑中神经肽Y(neuropeptide Y,NPY)mRNA表达的影响。方法:高脂饲料构建肥胖大鼠模型,肥胖大鼠和普通大鼠分别随机分为生理盐水组、小剂量组和大剂量组,分别连续20天腹腔注射生理盐水0.2 ml/100 g、琥珀酸氢化可的松5 mg/kg和15 mg/kg。并观察其摄食变化,用实时荧光定量PCR方法,检测大鼠下丘脑中NPY mRNA的表达水平。结果:应用大剂量糖皮质激素后普通大鼠和肥胖大鼠的摄食量低于盐水组(P<0.05);应用大剂量糖皮质激素后普通大鼠和肥胖大鼠的下丘脑中NPY mRNA的表达水平低于盐水组(P<0.01);应用小剂量糖皮质激素后普通大鼠的下丘脑中NPY mRNA的表达水平低于盐水组(P<0.01)。结论:大剂量糖皮质激素使普通大鼠和肥胖大鼠食欲下降,抑制肥胖大鼠和普通大鼠NPY mRNA的表达;小剂量糖皮质激素抑制普通大鼠NPY mRNA的表达,NPY在食欲调节中起重要作用。
- 肥胖 /
- 糖皮质激素类 /
- 神经肽Y /
- 食欲 /
- 大鼠
Abstract: Objective: To investigate the effects of glucocorticoid on neuropeptide Y(NPY) gene expression of the hypothalamus in obese and nonobese rats.Methods: Obese rat model was established by high-fat diet.Both the obese and nonobese rats were randomly assigned to normal saline(NS) group,low dose and high dose of glucocorticoid groups,and were administered intraperitoneal injection with normal saline 0.2 ml/100 g,hydrocortisone sodium succinate 5 mg/kg and 15 mg/kg,respectively,for 20 days.Their food intake was measured.Real-time quantitative PCR was used to measure the expression of NPY mRNA in the hypothalamus of the rats.Results: After high dose of glucocorticoid injection,the food intake of the obese and nonobese rats was lower than that of the NS group(P<0.05);the mRNA expressions of NPY in the hypothalamus of the obese and nonobese rats was lower than that of the NS group(P<0.01);the expression of NPY mRNA of the nonobese rats in the low dose group was significantly lower than that of the NS group(P<0.01).Conclusions: High dose of glucocorticoid may inhibit the appetite and the NPY mRNA expression in the hypothalamus of the obese and nonobese rats;low dose of glucocorticoid can inhibit the NPY mRNA expression in the hypothalamus of nonobese rats.NPY plays an important role in regulation of the appetite of rats.
- obesity /
- glucocorticoids /
- neuropehtide Y /
- appetite /
- rats

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糖皮质激素对肥胖大鼠和普通大鼠下丘脑神经肽Y mRNA的影响

作者简介: 范艳萍(1980-).女.硕士,研究生

1. 蚌埠医学院第一附属医院内分泌科, 安徽蚌埠 233004;
2. 蚌埠医学院第一附属医院中心实验室, 安徽蚌埠 233004

收稿日期: 2008-07-11

基金项目: 国家自然科学基金资助项目(No.30440026)

关键词:

摘要: 目的:研究糖皮质激素对肥胖大鼠和普通大鼠下丘脑中神经肽Y(neuropeptide Y,NPY)mRNA表达的影响。方法:高脂饲料构建肥胖大鼠模型,肥胖大鼠和普通大鼠分别随机分为生理盐水组、小剂量组和大剂量组,分别连续20天腹腔注射生理盐水0.2 ml/100 g、琥珀酸氢化可的松5 mg/kg和15 mg/kg。并观察其摄食变化,用实时荧光定量PCR方法,检测大鼠下丘脑中NPY mRNA的表达水平。结果:应用大剂量糖皮质激素后普通大鼠和肥胖大鼠的摄食量低于盐水组(P<0.05);应用大剂量糖皮质激素后普通大鼠和肥胖大鼠的下丘脑中NPY mRNA的表达水平低于盐水组(P<0.01);应用小剂量糖皮质激素后普通大鼠的下丘脑中NPY mRNA的表达水平低于盐水组(P<0.01)。结论:大剂量糖皮质激素使普通大鼠和肥胖大鼠食欲下降,抑制肥胖大鼠和普通大鼠NPY mRNA的表达;小剂量糖皮质激素抑制普通大鼠NPY mRNA的表达,NPY在食欲调节中起重要作用。

English Abstract

Influence of glucocorticoids on neuropeptide Y mRNA expression in hypothalamus of obese and nonobese rats

1. Department of Endocrinotogy, The First Affiliated Hospital of Bengbu Medical College, Bengbu Anhui 233004, China;
2. Department of Central Laboratories, The First Affiliated Hospital of Bengbu Medical College, Bengbu Anhui 233004, China

Received Date: 2008-07-11

Abstract: Objective: To investigate the effects of glucocorticoid on neuropeptide Y(NPY) gene expression of the hypothalamus in obese and nonobese rats.Methods: Obese rat model was established by high-fat diet.Both the obese and nonobese rats were randomly assigned to normal saline(NS) group,low dose and high dose of glucocorticoid groups,and were administered intraperitoneal injection with normal saline 0.2 ml/100 g,hydrocortisone sodium succinate 5 mg/kg and 15 mg/kg,respectively,for 20 days.Their food intake was measured.Real-time quantitative PCR was used to measure the expression of NPY mRNA in the hypothalamus of the rats.Results: After high dose of glucocorticoid injection,the food intake of the obese and nonobese rats was lower than that of the NS group(P<0.05);the mRNA expressions of NPY in the hypothalamus of the obese and nonobese rats was lower than that of the NS group(P<0.01);the expression of NPY mRNA of the nonobese rats in the low dose group was significantly lower than that of the NS group(P<0.01).Conclusions: High dose of glucocorticoid may inhibit the appetite and the NPY mRNA expression in the hypothalamus of the obese and nonobese rats;low dose of glucocorticoid can inhibit the NPY mRNA expression in the hypothalamus of nonobese rats.NPY plays an important role in regulation of the appetite of rats.