4-1BBL/4-1BB信号对系统性红斑狼疮患者外周血淋巴细胞活化的影响

高元元; 吴俊英; 李柏青; 王慧; 梁友宝; 梁华

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4-1BBL/4-1BB信号对系统性红斑狼疮患者外周血淋巴细胞活化的影响

高元元 , 吴俊英 , 李柏青 , 王慧 , 梁友宝 , 梁华

文章导航 > 蚌埠医学院学报 > 2011, 36 > 11: (1177-1181)

引用本文:

Citation:

4-1BBL/4-1BB信号对系统性红斑狼疮患者外周血淋巴细胞活化的影响

1. 蚌埠医学院临床检验诊断学实验中心, 安徽蚌埠 233030;
2. 蚌埠医学院免疫学教研室, 安徽蚌埠 233030

作者简介: 高元元(1983-),女,硕士研究生,检验师.

通讯作者: 吴俊英, 教授.email:jywu8191@126.com ;

中图分类号: R593.24

The effect of costimulatory signal 4-1 BBL/4-1 BB on the activation of peripheral blood lymphocytes with systemic lupus erythematosus

1. Research Center of Clinical Laboratory Science, Bengbu Medical College, Bengbu Anhui 233030, China;
2. Department of Immunology, Bengbu Medical College, Bengbu Anhui 233030, China

Corresponding author: WU Jun-ying, 教授.email:jywu8191@126.com ;

CLC number: R593.24

摘要: 目的:探讨4-1BBL/4-1BB信号对系统性红斑狼疮(SLE)患者外周血淋巴细胞活化及共刺激分子表达的影响。方法:用CD3单抗刺激SLE患者外周血单个核细胞,并用抗4-1BBL单抗阻断4-1BBL/4-1BB共刺激信号,流式细胞术检测阻断前后T、B细胞上4-1BB、CD40L、4-1BBL、CD40以及CD69表达水平。结果:SLE患者T细胞上CD69表达明显高于正常对照组(P<0.01);SLE患者外周血T细胞上4-1BB、CD40L和B细胞上4-1BBL、CD40表达明显高于正常对照组(P<0.01),活化后T细胞上共刺激分子表达升高更加明显(P<0.01);抗4-1BBL单抗阻断后4-1BB、CD40L表达明显下降(P<0.01),B细胞上CD40表达下降,与SLE未活化组和活化组比较差异有统计学意义(P<0.05)。结论:SLE患者外周血T、B细胞活化水平升高,4-1BBL/4-1BB信号对其活化状态维持可以起重要作用。
- 红斑狼疮,系统性 /
- 4-1BB /
- CD40L /
- CD69
Abstract: Objective:To investigate the effect of costimulatory signal 4-1BBL/4-1 BB in the activation of the T lymphocytes and the expression of costimulatory molecule of patients with (SLE). Methods:The peripheral blood mononuclear cells (PBMCs) were stimulated with anti-CD3 and the costimulatory signal of 4-1BBL/4-1BB was blocked by anti-4-1BBL. The expressions of 4-1BB, CD40L,4-1BBL,CD40 and CD69 of T cell and B cell before blocked and after blocked by anti-4-1BBL were detected by flow cytometry. Results:The expression of CD69 on T cells of SLE patients showed significant difference(P<0.01) with healthy controls. The expressions of 4-1BB,CD40L,4-1BBL and CD40 on the lymphocytes of SLE patients were significantly higher than that of normal control(P<0.01). There were obviously increased expression of 4-1BB and CD40L on the T lymphocytes stimulated by anti-CD3 antibody in SLE patients than healthy controls (P<0.01). With the anti-4-1BBL monoantibody blocked, there were decreased expression of 4-1BB CD40L (P<0.01). The expression of CD40 on B cell was significantly decreased, which is less than SLE unstimulted group and SLE stimulated group(P<0.05). Conclusions:T cell and B cell activation level of SLE patients were increased and 4-1BBL/4-1BB signals play a role in keeping the activation.
- lupus erythematosu,systemic /
- 4-1BB /
- CD40L /
- CD69

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4-1BBL/4-1BB信号对系统性红斑狼疮患者外周血淋巴细胞活化的影响

通讯作者: 吴俊英, 教授.email:jywu8191@126.com;

作者简介: 高元元(1983-),女,硕士研究生,检验师.

1. 蚌埠医学院临床检验诊断学实验中心, 安徽蚌埠 233030;
2. 蚌埠医学院免疫学教研室, 安徽蚌埠 233030

收稿日期: 2010-11-26

关键词:

红斑狼疮,系统性 /
4-1BB /
CD40L /
CD69

摘要: 目的:探讨4-1BBL/4-1BB信号对系统性红斑狼疮(SLE)患者外周血淋巴细胞活化及共刺激分子表达的影响。方法:用CD3单抗刺激SLE患者外周血单个核细胞,并用抗4-1BBL单抗阻断4-1BBL/4-1BB共刺激信号,流式细胞术检测阻断前后T、B细胞上4-1BB、CD40L、4-1BBL、CD40以及CD69表达水平。结果:SLE患者T细胞上CD69表达明显高于正常对照组(P<0.01);SLE患者外周血T细胞上4-1BB、CD40L和B细胞上4-1BBL、CD40表达明显高于正常对照组(P<0.01),活化后T细胞上共刺激分子表达升高更加明显(P<0.01);抗4-1BBL单抗阻断后4-1BB、CD40L表达明显下降(P<0.01),B细胞上CD40表达下降,与SLE未活化组和活化组比较差异有统计学意义(P<0.05)。结论:SLE患者外周血T、B细胞活化水平升高,4-1BBL/4-1BB信号对其活化状态维持可以起重要作用。

English Abstract

The effect of costimulatory signal 4-1 BBL/4-1 BB on the activation of peripheral blood lymphocytes with systemic lupus erythematosus

Corresponding author: WU Jun-ying, 教授.email:jywu8191@126.com

1. Research Center of Clinical Laboratory Science, Bengbu Medical College, Bengbu Anhui 233030, China;
2. Department of Immunology, Bengbu Medical College, Bengbu Anhui 233030, China

Received Date: 2010-11-26

Abstract: Objective:To investigate the effect of costimulatory signal 4-1BBL/4-1 BB in the activation of the T lymphocytes and the expression of costimulatory molecule of patients with (SLE). Methods:The peripheral blood mononuclear cells (PBMCs) were stimulated with anti-CD3 and the costimulatory signal of 4-1BBL/4-1BB was blocked by anti-4-1BBL. The expressions of 4-1BB, CD40L,4-1BBL,CD40 and CD69 of T cell and B cell before blocked and after blocked by anti-4-1BBL were detected by flow cytometry. Results:The expression of CD69 on T cells of SLE patients showed significant difference(P<0.01) with healthy controls. The expressions of 4-1BB,CD40L,4-1BBL and CD40 on the lymphocytes of SLE patients were significantly higher than that of normal control(P<0.01). There were obviously increased expression of 4-1BB and CD40L on the T lymphocytes stimulated by anti-CD3 antibody in SLE patients than healthy controls (P<0.01). With the anti-4-1BBL monoantibody blocked, there were decreased expression of 4-1BB CD40L (P<0.01). The expression of CD40 on B cell was significantly decreased, which is less than SLE unstimulted group and SLE stimulated group(P<0.05). Conclusions:T cell and B cell activation level of SLE patients were increased and 4-1BBL/4-1BB signals play a role in keeping the activation.