全自动酶免分析仪加样中拖带阳性的解决方法探讨

朱安友; 王凤超; 杨萍; 葛鑫; 郭晖; 刘雅

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全自动酶免分析仪加样中拖带阳性的解决方法探讨

朱安友 , 王凤超 , 杨萍 , 葛鑫 , 郭晖 , 刘雅

文章导航 > 蚌埠医学院学报 > 2010, 35 > 8: (817-819)

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全自动酶免分析仪加样中拖带阳性的解决方法探讨

蚌埠医学院第一附属医院检验科, 安徽蚌埠 233004

作者简介: 朱安友(1968-),男,主管检验师.

中图分类号: R392.33

A study on the resolution of sampling probe towing positive in automatic enzyme immunoassay instrument

Department of Clinical Laboratories, The First Affiliated Hospital of Bengbu Medical College, Bengbu Anhui 233004, China
CLC number: R392.33

摘要: 目的:探讨全自动酶免分析仪加样中拖带阳性的解决办法。方法:收集常规筛查中的人体免疫缺陷病毒(human immunodeficiency virus,HIV)抗体、梅毒螺旋体(treponema pallidum,TP)抗体、丙型肝炎病毒(hepatitis C virus,HCV)抗体和乙肝表面抗原(hepatitis B surface antigen,HBsAg)强阳性标本各1份;分别在使用去离子水和0.1% Tween-20弱碱性溶液作为系统冲洗液的情况下,用RMP 200型全自动酶免分析仪做阳性拖带模拟试验;观察0.1% Tween-20弱碱性溶液作为系统冲洗液在消除加样针拖带阳性中的作用,以及其对酶免试验的影响。结果:在拖带模拟试验中,以去离子水作为系统冲洗液时,HIV抗体、TP抗体、HCV抗体和HBsAg强阳性标本都出现了阳性拖带现象,其中HIV抗体拖带阳性最为显著,有6孔被拖带阳性,HBsAg只出现1孔拖带阳性;而以0.1% Tween-20弱碱性溶液作为系统冲洗液时,HIV抗体、TP抗体、HCV抗体和HBsAg均无阳性拖带孔出现;并且在使用2种不同系统冲洗液情况下,相同检测项目之间的质控品和对照品的实验结果差异均无统计学意义(P>0.05)。结论:0.1% Tween-20弱碱性溶液作为系统冲洗液能够避免全自动酶免分析仪加样中拖带阳性现象的发生,并且对酶免试验结果没有显著影响。
- 免疫酶技术 /
- 自动分析 /
- 加样针 /
- 拖带阳性 /
- Tween-20
Abstract: Objective: To investigate the resolution of sampling probe towing positive in automatic enzyme immunoassay instrument.Methods: Anti-human immunodeficiency virus (HIV),anti-treponema pallidum (TP),anti-hepatitis C virus (HCV) or hepatitis B surface antigen (HBsAg) strong positive specimens were collected from routine screening test.The towing positive simulation test was performed in RMP 200 automatic enzyme immunoassay under use of demineralized water or 0.1% Tween-20 weak alkaline solution for sampling probe irrigation solutions conditions.By comparison of the two groups results,it was observed that 0.1% Tween-20 weak alkaline solution in role of avoid sampling probe towing positive and it effect on ELISA.Results: It was found that every of anti-HIV,anti-TP,anti-HCV,and HBsAg sepecimen had experienced towing positive under use of demineralized water for sampling probe irrigation solutions.Among them,anti-HIV towing positive was most obvious and had 6 towing positive holes;while HBsAg appeared only a towing weak positive hole.In contrast to demineralized water,all towing positive phenomena of anti-HIV,anti-TP,anti-HCV and HBsAg strong positive specimens were avoided under use of 0.1% Tween-20 weak alkaline solution for sampling probe irrigation solutions.Moreover,the results of quality control serum,positive and negative control under two different irrigation solutions had no significant difference for the same test item (P>0.05).Conclusions: The use of 0.1% Tween-20 weak alkaline solution for sampling probe irrigation solutions could avoid appearance of sampling probe towing positive in automatic enzyme immunoassay instrument and it had no significant effect on ELISA.
- immunoenzyme technique /
- autoanalysis /
- sampling probe /
- towing positive /
- Tween-20

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全自动酶免分析仪加样中拖带阳性的解决方法探讨

作者简介: 朱安友(1968-),男,主管检验师.

蚌埠医学院第一附属医院检验科, 安徽蚌埠 233004

收稿日期: 2009-12-30

关键词:

摘要: 目的:探讨全自动酶免分析仪加样中拖带阳性的解决办法。方法:收集常规筛查中的人体免疫缺陷病毒(human immunodeficiency virus,HIV)抗体、梅毒螺旋体(treponema pallidum,TP)抗体、丙型肝炎病毒(hepatitis C virus,HCV)抗体和乙肝表面抗原(hepatitis B surface antigen,HBsAg)强阳性标本各1份;分别在使用去离子水和0.1% Tween-20弱碱性溶液作为系统冲洗液的情况下,用RMP 200型全自动酶免分析仪做阳性拖带模拟试验;观察0.1% Tween-20弱碱性溶液作为系统冲洗液在消除加样针拖带阳性中的作用,以及其对酶免试验的影响。结果:在拖带模拟试验中,以去离子水作为系统冲洗液时,HIV抗体、TP抗体、HCV抗体和HBsAg强阳性标本都出现了阳性拖带现象,其中HIV抗体拖带阳性最为显著,有6孔被拖带阳性,HBsAg只出现1孔拖带阳性;而以0.1% Tween-20弱碱性溶液作为系统冲洗液时,HIV抗体、TP抗体、HCV抗体和HBsAg均无阳性拖带孔出现;并且在使用2种不同系统冲洗液情况下,相同检测项目之间的质控品和对照品的实验结果差异均无统计学意义(P>0.05)。结论:0.1% Tween-20弱碱性溶液作为系统冲洗液能够避免全自动酶免分析仪加样中拖带阳性现象的发生,并且对酶免试验结果没有显著影响。

English Abstract

A study on the resolution of sampling probe towing positive in automatic enzyme immunoassay instrument

Department of Clinical Laboratories, The First Affiliated Hospital of Bengbu Medical College, Bengbu Anhui 233004, China

Received Date: 2009-12-30

Abstract: Objective: To investigate the resolution of sampling probe towing positive in automatic enzyme immunoassay instrument.Methods: Anti-human immunodeficiency virus (HIV),anti-treponema pallidum (TP),anti-hepatitis C virus (HCV) or hepatitis B surface antigen (HBsAg) strong positive specimens were collected from routine screening test.The towing positive simulation test was performed in RMP 200 automatic enzyme immunoassay under use of demineralized water or 0.1% Tween-20 weak alkaline solution for sampling probe irrigation solutions conditions.By comparison of the two groups results,it was observed that 0.1% Tween-20 weak alkaline solution in role of avoid sampling probe towing positive and it effect on ELISA.Results: It was found that every of anti-HIV,anti-TP,anti-HCV,and HBsAg sepecimen had experienced towing positive under use of demineralized water for sampling probe irrigation solutions.Among them,anti-HIV towing positive was most obvious and had 6 towing positive holes;while HBsAg appeared only a towing weak positive hole.In contrast to demineralized water,all towing positive phenomena of anti-HIV,anti-TP,anti-HCV and HBsAg strong positive specimens were avoided under use of 0.1% Tween-20 weak alkaline solution for sampling probe irrigation solutions.Moreover,the results of quality control serum,positive and negative control under two different irrigation solutions had no significant difference for the same test item (P>0.05).Conclusions: The use of 0.1% Tween-20 weak alkaline solution for sampling probe irrigation solutions could avoid appearance of sampling probe towing positive in automatic enzyme immunoassay instrument and it had no significant effect on ELISA.