三氧化二砷对乳腺癌细胞长链非编码RNA HOX转录反义RNA的作用
Effect of arsenic trioxide on the long chain noncoding HOTAIR expression in breast cancer cells
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摘要: 目的：探讨长链非编码RNA HOX转录反义RNA(HOTAIR)在乳腺癌组织和细胞中的表达情况及三氧化二砷(As2O3)对HOTAIR基因表达的影响。方法：实时定量PCR检测在乳腺癌癌旁组织与癌组织中长链非编码RNA HOTAIR mRNA的表达，筛选高表达HOTAIR的乳腺癌细胞株，检测As2O3对HOTAIR mRNA表达的作用。先观察比较乳腺癌与癌旁组织中HOTAIR的表达，再检测乳腺癌细胞株中HOTAIR的表达，以及As2O3作用后MCF-7细胞中HOTAIR的表达变化。结果：乳腺癌组织中HOTAIR mRNA表达量为0.011±0.005，癌旁组织未见表达；乳腺癌细胞株SKBR-3和MAD-MB-231细胞均未表达HOTAIR mRNA，MCF-7细胞株高表达HOTAIR mRNA(1±0.236)；在As2O3作用下，MCF-7细胞中HOTAIR mRNA表达较空白组明显下降(P＜0.01)。结论：HOTAIR与乳腺癌的发展可能相关，As2O3可以抑制其表达。
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关键词:
- 乳腺肿瘤 /
- 长链非编码RNA /
- HOX转录反义RNA /
- 三氧化二砷
Abstract: Objective:To investigate the expression of long-chain non-coding HOX transcript antisense RNA(HOTAIR) in breast cancer tissues and cells,and effects of arsenic trioxide(As2O3) on its expression.Methods:The expressions of long-chain non-coding HOTAIR mRNA in breast carcer tissue and pericarcinomatous tissue were detected using real-time fluorescence quantitative PCR.Breast cancer cell lines with high expression HOTAIR was screened,the effect of As2O3 on the expression of HOTAIR mRNA was detected.The expressions of HOTAIR between breast cancer tissue,pericarcinomatous tissue and breast cancer cell lines were compared.The HOTAIR expression in MCF-7 cells treated with As2O3 was detected.Results:The expression quantity of HOTAIR mRNA in breast cancer was 0.011?0.005,no expression of HOTAIR was found in pericarcinomatous tissue.The HOTAIR mRNA expression was not found in SKBR-3 and MAD-MB-231 cells.The high expression of HOTAIR mRNA(1?0.236) was detected in MCF-7 cells.The expression level of HOTAIR mRNA in MCF-7 cells treated with As2O3 was significantly lower than that in blank group(PConclusions:The HOTAIR expression is associated with the incidence of breast cancer,and As2O3 can inhibit its expression. -
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