条件性恐惧消退大鼠内侧前额叶皮层边缘下区CaMKⅡ的变化

焦东亮; 许华山; 宋红梅; 王立金

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条件性恐惧消退大鼠内侧前额叶皮层边缘下区CaMKⅡ的变化

焦东亮 , 许华山 , 宋红梅 , 王立金

文章导航 > 蚌埠医学院学报 > 2013, 37 > 4: (381-383)

引用本文:

Citation:

条件性恐惧消退大鼠内侧前额叶皮层边缘下区CaMKⅡ的变化

1.
1. 蚌埠医学院临床心理学教研室, 安徽蚌埠 233030;
2.
2. 蚌埠医学院附属安民医院, 安徽怀远 233400

作者简介: 焦东亮(1973－ ),男,副教授.

通讯作者: 许华山, jdl3925697@163.com ;

基金项目:
安徽省高校省级科学研究基金资助项目(KJ2011B099)

The expression of calmodulin kinase Ⅱ in infra-limbic cortex of medial prefrontal cortex of rats after fear extinction

1.
1. Department of Clinical Psychology, Bengbu Medical College, Bengbu Anhui 233030;
2.
2. Anmin Hospital Affiliated to Bengbu Medical College, Huaiyuan Anhui 233400, China

Corresponding author: XU Hua-shan, jdl3925697@163.com ;

摘要: 目的:探讨条件性恐惧消退早期(1周内)大鼠记忆保持成绩和内侧前额叶皮层边缘下区钙调素依赖性蛋白激酶Ⅱ(CaMKⅡ)的动态变化。方法:将70只成年雄性SD大鼠随机分为正常对照组、恐惧消退组和恐惧对照组,其中恐惧对照组为条件恐惧建立后不进行消退训练,恐惧消退组为条件恐惧建立24 h后进行消退训练,采用以声音为提示的足底电击建立大鼠恐惧记忆,然后仅给予声音信号进行恐惧消退训练。在恐惧消退后第1、3、7天分别进行消退保持测试,并在各时间点取边缘下区标本进行CaMKⅡ的免疫组织化学检测。结果:在消退保持过程中,恐惧对照组和恐惧消退组大鼠消退成绩整体呈上升趋势,但均低于正常对照组,恐惧消退组消退成绩好于恐惧对照组(P0.01);恐惧对照组和恐惧消退组大鼠边缘下区CaMKⅡ免疫反应阳性物质逐渐增加,但均低于正常对照组,第1天无明显不同,第3、7天恐惧消退组CaMKⅡ免疫反应阳性物质均低于恐惧对照组(P0.01)。结论:恐惧消退训练后3～7 d,边缘下区CaMKⅡ水平变化与恐惧记忆消退训练相关。
- 应激障碍 /
- 创伤后 /
- 恐惧消退 /
- 钙调素依赖性蛋白激酶Ⅱ /
- 前额叶皮层 /
- 边缘下区
Abstract: Objective:To observe the expression change of calmodulin kinaseⅡ(CaMK Ⅱ)in infra-limbic cortex of medial prefrontal cortex of adult rats in the early stage of fear extinction.Methods:Seventy rats were randomly divided into normal control,fear extinction and fear control group.The fear extinction training was not implemented in fear control group.The fear extinction group were trained to extinct fear with sound signal at 24 hours after fear established by sound hint of planter electric shock.The expressions of CaMK Ⅱ in infra-limbic cortex were detected by immunohistochemistry at 1,3 and 7 days after fear extinction.Results:The expression of CaMK Ⅱ in fear extinction and fear control group increased gradually,which was lower than that in normal control group.The fear extinction group were better than that in fear control group(P0.01).The immunoreactivities of CaMK Ⅱin infra-limbic cortex of fear extinction and fear control group increased gradually,which were lower than that in normal control group.The immunoreactivities of CaMK Ⅱ in fear extinction group were lower than that in fear control group at 3 and 7 days after fear extinction(P0.01),but no significant difference at day 1.Conclusions:The CaMK Ⅱ level in infra-limbic cortex is consistent with the extinction memory at 3 and 7 days after fear extinction training.
- stress disorder /
- post-traumatic /
- fear extinction /
- calmodulin kinaseⅡ /
- medial prefrontal cortex /
- infra-limbic cortex

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条件性恐惧消退大鼠内侧前额叶皮层边缘下区CaMKⅡ的变化

通讯作者: 许华山, jdl3925697@163.com;

作者简介: 焦东亮(1973－ ),男,副教授.

1. 1. 蚌埠医学院临床心理学教研室, 安徽蚌埠 233030;
2. 2. 蚌埠医学院附属安民医院, 安徽怀远 233400

收稿日期: 2012-04-25
网络出版日期: 2013-04-15

基金项目: 安徽省高校省级科学研究基金资助项目(KJ2011B099)

关键词:

摘要: 目的:探讨条件性恐惧消退早期(1周内)大鼠记忆保持成绩和内侧前额叶皮层边缘下区钙调素依赖性蛋白激酶Ⅱ(CaMKⅡ)的动态变化。方法:将70只成年雄性SD大鼠随机分为正常对照组、恐惧消退组和恐惧对照组,其中恐惧对照组为条件恐惧建立后不进行消退训练,恐惧消退组为条件恐惧建立24 h后进行消退训练,采用以声音为提示的足底电击建立大鼠恐惧记忆,然后仅给予声音信号进行恐惧消退训练。在恐惧消退后第1、3、7天分别进行消退保持测试,并在各时间点取边缘下区标本进行CaMKⅡ的免疫组织化学检测。结果:在消退保持过程中,恐惧对照组和恐惧消退组大鼠消退成绩整体呈上升趋势,但均低于正常对照组,恐惧消退组消退成绩好于恐惧对照组(P0.01);恐惧对照组和恐惧消退组大鼠边缘下区CaMKⅡ免疫反应阳性物质逐渐增加,但均低于正常对照组,第1天无明显不同,第3、7天恐惧消退组CaMKⅡ免疫反应阳性物质均低于恐惧对照组(P0.01)。结论:恐惧消退训练后3～7 d,边缘下区CaMKⅡ水平变化与恐惧记忆消退训练相关。

English Abstract

The expression of calmodulin kinase Ⅱ in infra-limbic cortex of medial prefrontal cortex of rats after fear extinction

Corresponding author: XU Hua-shan, jdl3925697@163.com

1. 1. Department of Clinical Psychology, Bengbu Medical College, Bengbu Anhui 233030;
2. 2. Anmin Hospital Affiliated to Bengbu Medical College, Huaiyuan Anhui 233400, China

Received Date: 2012-04-25
Available Online: 2013-04-15

Abstract: Objective:To observe the expression change of calmodulin kinaseⅡ(CaMK Ⅱ)in infra-limbic cortex of medial prefrontal cortex of adult rats in the early stage of fear extinction.Methods:Seventy rats were randomly divided into normal control,fear extinction and fear control group.The fear extinction training was not implemented in fear control group.The fear extinction group were trained to extinct fear with sound signal at 24 hours after fear established by sound hint of planter electric shock.The expressions of CaMK Ⅱ in infra-limbic cortex were detected by immunohistochemistry at 1,3 and 7 days after fear extinction.Results:The expression of CaMK Ⅱ in fear extinction and fear control group increased gradually,which was lower than that in normal control group.The fear extinction group were better than that in fear control group(P0.01).The immunoreactivities of CaMK Ⅱin infra-limbic cortex of fear extinction and fear control group increased gradually,which were lower than that in normal control group.The immunoreactivities of CaMK Ⅱ in fear extinction group were lower than that in fear control group at 3 and 7 days after fear extinction(P0.01),but no significant difference at day 1.Conclusions:The CaMK Ⅱ level in infra-limbic cortex is consistent with the extinction memory at 3 and 7 days after fear extinction training.