高浓度D-二聚体检测时稀释倍数的探讨

钟政荣; 徐云侠; 田万林; 宫继勇; 刘蕴霞; 张伦军

高浓度D-二聚体检测时稀释倍数的探讨

1.
1. 蚌埠医学院第一附属医院检验科, 安徽蚌埠 233004;
2.
2. 蚌埠医学院第一附属医院神经内科, 安徽蚌埠 233004

作者简介: 钟政荣(1972－ ),博士,副主任检验师.

基金项目:
安徽省教育厅自然科学研究重点资助项目(KJ2011A205)

Optimal dilution of high concentration D-dimer in D-dimer assay

1.
1. Department of Clinical Laboratory, The First Affiliated Hospital of Bengbu Medical College, Bengbu Anhui 233004, China;
2.
2. Department of Neurology, The First Affiliated Hospital of Bengbu Medical College, Bengbu Anhui 233004, China

摘要: 目的:分析高浓度D-二聚体(D-D)血浆稀释8倍时的错误结果,探讨高浓度D-D血浆检测时的最适稀释倍数。方法:将D-D标准血浆稀释成连续的6个稀释度进行标准曲线的制备,再将D-D标准血浆和高浓度血浆分别在机内和机外稀释2倍、4倍、8倍,再在定标后的血凝仪上进行检测,D-D报告结果机内模式仪器自动乘以稀释倍数,机外需人工乘以稀释倍数换算,最后比较机内和机外2种稀释模式的结果差异以及各稀释倍数与原液的结果差异。结果:D-D标准曲线呈一S型曲线。机内8倍稀释与机外2倍稀释后的D-D结果差异均有统计学意义(P0.01),但2倍稀释与原液结果差异无统计学意义(P0.05),2种稀释模式下相同稀释度之间的结果差异亦均无统计学意义(P0.05)。结论:高浓度D-D血浆稀释倍数愈大,结果差异愈明显,选择能够检出的最小稀释倍数是高浓度D-D检验结果的质量保证。
- D-二聚体 /
- 稀释 /
- 标准曲线
Abstract: Objective:To analyze the reasons for unreliable outcome resulting from the plasmas with high D-dimer(D-D) concentration diluted to 8 times,and explore the optimal dilution of the plasmas for D-D test.Methods:The D-D standard curve was derived from the six concentration levels of D-D standard plasma by a customary way.The standards and the patient plasma were diluted to 2 times,4 times and 8 times in a coagulation analyzer or by manual,and then they were analyzed in a calibrated analyzer;The results in the analyzer and by manual were statistically compared.Results:The standard curve of D-D was similar to the letter S.The D-D concentration when the plasma was diluted to 8 times in the analyzer was decreased significantly(P0.01),compared with that when diluted to 2 times by manual.However,there was not statistical difference between the plasma and its dilution to 2 times or between the same dilutions in both modes(P0.05).Conclusions:The higher the dilution is,the more obvious the difference will be.Therefore,the plasma with high D-D concentration should be diluted as less times as it can be determined in order to assure the reliability of D-D value.
- D-dimer /
- dilution /
- standard curve

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高浓度D-二聚体检测时稀释倍数的探讨

作者简介: 钟政荣(1972－ ),博士,副主任检验师.

Optimal dilution of high concentration D-dimer in D-dimer assay

计量

高浓度D-二聚体检测时稀释倍数的探讨

作者简介: 钟政荣(1972－ ),博士,副主任检验师.

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Optimal dilution of high concentration D-dimer in D-dimer assay

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高浓度D-二聚体检测时稀释倍数的探讨

作者简介: 钟政荣(1972－ ),博士,副主任检验师.

Optimal dilution of high concentration D-dimer in D-dimer assay

计量

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高浓度D-二聚体检测时稀释倍数的探讨

作者简介: 钟政荣(1972－ ),博士,副主任检验师.

English Abstract

Optimal dilution of high concentration D-dimer in D-dimer assay

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目录