医院感染非发酵革兰阴性杆菌的分布与耐药性分析

王锦; 李峰; 郭普; 张凡; 谢昌霞; 钟政荣

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医院感染非发酵革兰阴性杆菌的分布与耐药性分析

王锦 , 李峰 , 郭普 , 张凡 , 谢昌霞 , 钟政荣

文章导航 > 蚌埠医学院学报 > 2013, 37 > 6: (756-758)

引用本文:

Citation:

医院感染非发酵革兰阴性杆菌的分布与耐药性分析

1.
蚌埠医学院第一附属医院检验科

作者简介: 王锦(1982－)，女，检验师．

基金项目:

Distribution and drug resistance of clinical common non-fermentative gram-negative bacilli

1.
Department of Clinical Laboratory, The First Affiliated Hospital of Bengbu Medical College, Bengbu Anhui 233004, China

摘要: 目的：了解医院感染非发酵革兰阴性(G-)杆菌的临床分布特点及耐药性，为临床合理使用抗生素提供依据。方法：将各类临床标本中分离的728株非发酵G-杆菌进行统计和耐药性分析，细菌鉴定和药敏分析采用纸片琼脂扩散法和最小抑菌浓度法，按美国临床实验室标准化委员会制定的标准判断。结果：728株非发酵G-杆菌中，居前4位的依次为鲍曼不动杆菌330株，铜绿假单胞菌290株，嗜麦芽窄食单胞菌38株，洋葱伯克霍尔德菌38株。分布在呼吸系统为543株，以重症监护治疗病房最多，共308株。铜绿假单胞菌对亚胺培南、头孢哌酮/舒巴坦、哌拉西林/他唑巴坦耐药率低于30%；鲍曼不动杆菌耐药率最低的是米诺环素为31.2%，其次为头孢哌酮/舒巴坦为53.94%；嗜麦芽窄食单胞菌耐药率最低的是米诺环素为5.3%；洋葱伯克霍尔德菌对复方磺胺甲口恶唑、米诺环素耐药率较低，分别为21.05%、15.8%。结论：不同菌种的非发酵G-杆菌对药物的敏感性有较大差异。
- 非发酵菌 /
- 革兰阴性杆菌 /
- 耐药率 /
- 药物敏感性试验
Abstract: Objective:To monitor the distribution and drug resistance of clinical isolated common non-fermentative Gram-negative bacilli,so as to provide basis for appropriate use of antibiotics in clinic.Methods:The 728 strains common non-fermentative gram-negative bacilli were collected and their antimicrobial susceptibility was studied.The identification and drug susceptibility tests were detected by paper-disc agar-diffusion method and minimal inhibitory concentration method.The results were evaluated in accordance with the standard of American Clinical Laboratory Information Systems.Results:In the 728 strains non fermentative Gram-negative bacilli,the dominant four strains were Acinetobacter baumannii ( 330 strains ) firstly,Pseudomonas aeruginaosa ( 290 strains ),Stenotrophomonas maltophilia ( 38 strains) and Burkholderia cepacia ( also 38 strains).The main bacterias were most from sputum system,totally 543 strains and the most distribution were detected in intensive care unit ( 308 strains ).The resistant rates of Pseudomonas aeruginosa to imipenem cefoperaxone/sulbactam,piperacillin/tazobactam were all less than 30%.The resistant rates of Acinetobacter baumannii were 31.2% to minocycline,53.94% to cefoperaxone/sulbactam and all lower than others.The resistant rate of Stenotrophomonas maltophilia to minocycline was 5.3%.The resistant rates of Burkholderia cepacia to cotrimoxazole and minocycline were lower than others,21.05% and 15.8% respectively.Conclusions:Different kinds of nonfermentative Gram negative bacilli had a greater difference in drug sensitivity.
- nonfermentative bacteria /
- gram-negative bacilli /
- resistance rate /
- drug sensitivity rate

[1]	李忠兴,郑家齐,李家宏.诊断细菌学[M].香港:黄河文化出版社,1992:375.
[2] 杨启文,王辉,徐英春,等.2009 年中国 13 家教学医院院内感染病原菌的抗生素耐药性监测[J].中华检验医学杂志,2011,34(5):422-430.
[3] 兰会华,郭世辉,梁宏洁,等.重症监护病房常见非发酵菌的分布及耐药性分析[J].中华医院感染学杂志,2010,20(13):1957-1959.
[4] 陈淑兰,路娟,宋熙瑶,等.常见非发酵菌的临床分布与耐药性分析[J].中华医院感染学杂志,2008,18(4):589-591.
[5] 姜维苓.非发酵菌感染及其抗菌药物治疗[J].中国药事,2009,23(7):710-712.
[6] 罗燕萍,张军民,刘丽,等.洋葱伯克霍尔德菌对 29 种抗生素的耐药性分析[J].中华医院感染学杂志,2002,12(10):731-733.

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医院感染非发酵革兰阴性杆菌的分布与耐药性分析

作者简介: 王锦(1982－)，女，检验师．

1. 蚌埠医学院第一附属医院检验科

收稿日期: 2012-07-27
网络出版日期: 2013-06-15

基金项目:

关键词:

摘要: 目的：了解医院感染非发酵革兰阴性(G-)杆菌的临床分布特点及耐药性，为临床合理使用抗生素提供依据。方法：将各类临床标本中分离的728株非发酵G-杆菌进行统计和耐药性分析，细菌鉴定和药敏分析采用纸片琼脂扩散法和最小抑菌浓度法，按美国临床实验室标准化委员会制定的标准判断。结果：728株非发酵G-杆菌中，居前4位的依次为鲍曼不动杆菌330株，铜绿假单胞菌290株，嗜麦芽窄食单胞菌38株，洋葱伯克霍尔德菌38株。分布在呼吸系统为543株，以重症监护治疗病房最多，共308株。铜绿假单胞菌对亚胺培南、头孢哌酮/舒巴坦、哌拉西林/他唑巴坦耐药率低于30%；鲍曼不动杆菌耐药率最低的是米诺环素为31.2%，其次为头孢哌酮/舒巴坦为53.94%；嗜麦芽窄食单胞菌耐药率最低的是米诺环素为5.3%；洋葱伯克霍尔德菌对复方磺胺甲口恶唑、米诺环素耐药率较低，分别为21.05%、15.8%。结论：不同菌种的非发酵G-杆菌对药物的敏感性有较大差异。

English Abstract

Distribution and drug resistance of clinical common non-fermentative gram-negative bacilli

1. Department of Clinical Laboratory, The First Affiliated Hospital of Bengbu Medical College, Bengbu Anhui 233004, China

Received Date: 2012-07-27
Available Online: 2013-06-15

Abstract: Objective:To monitor the distribution and drug resistance of clinical isolated common non-fermentative Gram-negative bacilli,so as to provide basis for appropriate use of antibiotics in clinic.Methods:The 728 strains common non-fermentative gram-negative bacilli were collected and their antimicrobial susceptibility was studied.The identification and drug susceptibility tests were detected by paper-disc agar-diffusion method and minimal inhibitory concentration method.The results were evaluated in accordance with the standard of American Clinical Laboratory Information Systems.Results:In the 728 strains non fermentative Gram-negative bacilli,the dominant four strains were Acinetobacter baumannii ( 330 strains ) firstly,Pseudomonas aeruginaosa ( 290 strains ),Stenotrophomonas maltophilia ( 38 strains) and Burkholderia cepacia ( also 38 strains).The main bacterias were most from sputum system,totally 543 strains and the most distribution were detected in intensive care unit ( 308 strains ).The resistant rates of Pseudomonas aeruginosa to imipenem cefoperaxone/sulbactam,piperacillin/tazobactam were all less than 30%.The resistant rates of Acinetobacter baumannii were 31.2% to minocycline,53.94% to cefoperaxone/sulbactam and all lower than others.The resistant rate of Stenotrophomonas maltophilia to minocycline was 5.3%.The resistant rates of Burkholderia cepacia to cotrimoxazole and minocycline were lower than others,21.05% and 15.8% respectively.Conclusions:Different kinds of nonfermentative Gram negative bacilli had a greater difference in drug sensitivity.