埃兹蛋白在乳腺癌中的表达及意义

王明喜; 郑荣生; 承泽农; 汪子书; 高振远; 韩正全

埃兹蛋白在乳腺癌中的表达及意义

1.
1. 蚌埠医学院第一附属医院肿瘤内科;
2.
2. 蚌埠医学院第一附属医院病理科

作者简介: 王明喜(1973－)，男，硕士，主治医师．

基金项目:

Expression of Ezrin and its significance in breast cancer

1.
1. Department of Oncology;
2.
2. Department of Pathology, The First Affiliated Hospital of Bengbu Medical College, Bengbu Anhui 233004, China

摘要: 目的：探讨埃兹蛋白(Ezrin)在乳腺癌中的表达及其临床病理意义。方法：用免疫组织化学法(SP法)检测90例乳腺癌及10例乳腺腺病组织和10例乳腺导管内乳头状瘤组织Ezrin的表达情况。结果：Ezrin在乳腺癌、乳腺腺病、乳腺导管内乳头状瘤中的强阳性率分别为58.9%、10.0%和20.0%，差异有统计学意义(P0.01)。肿瘤淋巴结转移者Ezrin强阳性率高于无转移者(P0.01)，在肿瘤大小、TNM分期、年龄间的表达差异均无统计学意义(P0.05)。免疫组织化学显示Ezrin在乳腺癌中大部分表达定位于细胞质(62.2%)，部分表达定位于整个细胞膜(22.0%)与细胞膜顶端(15.8%)，而在乳腺腺病和乳腺导管内乳头状瘤中则主要表达定位于细胞膜顶端(7/9和6/9)，3组Ezrin表达定位差异有统计学意义(P0.01)。乳腺癌中Ezrin细胞膜顶端表达定位与淋巴结无转移、低级别分化、激素受体阳性均有一定关系(P0.05～P0.01);Ezrin细胞质表达定位与淋巴结转移、高级别分化、激素受体阴性亦均有一定关系(P0.05～P0.01);Ezrin细胞膜表达定位在人类表皮生长因子受体2阴阳性表达间差异有统计学意义(P0.05)。结论：Ezrin的表达增高和表达部位的变化在乳腺癌的发生、发展和转移过程中发挥着调控作用，可作为判断乳腺癌转移和预后的重要生物学指标。
- 乳腺肿瘤 /
- 埃兹蛋白 /
- 转移 /
- 免疫组织化学
Abstract: Objective: To investigate the expression of Ezrin and its significance in breast cancer.Methods:The Ezrin expressions in breast cancer tissue of 90 cases,adenosis tissue of 10 cases and breast intraductal papillary tumor tissue of 10 cases were detected by SP immunohistochemistry.Results:The strong positive expression rates of Ezrin in breast cancer tissue,adenosis tissue and breast intraductal papillary tumor tissue were 58.9%,10.0% and 20.0%,respectively,the differences of which were statistical significant(P 0.01).The expression level of Ezrin in cases with lymph node metastasis was higher than in whose without lymph node metastasis(P 0.01),and its expression differences in tumor size,TNM staging and age were not statistically significant (P0.05).The most expression position of Ezrin in breast cancer was in the cytoplasm(62.2%),part expression was in the membrane(22.0%) and cell membrane top(15.8%).The most expression position of Ezrin in adenosis tissue and breast intraductal papillary tumor tissue was in cell membrane top(7 /9 and 6 /9).The Ezrin expression differences of three groups were statistical significance(P0.01).The Ezrin expression in cell membrane top of breast cancer was related to the lymph node metastasis,differentiation stage and hormone receptor expression(P0.05 to P0.01).The Ezrin expression of in cytoplasm was related to the lymph node metastasis,high differentiation stage and hormone receptor negative expression(P0.05 to P0.01).The differences of Ezrin expression in membrane and HER2 expression were statistical significance (P0.05).Conclusions:The heightening expression and the expression position change of Ezrin play a regulating role on the occurrence,development and metastasis of breast cancer,which may be an important biological indicator in diagnosing the metastasis and prognosis of breast cancer.
- breast cancer /
- Ezrin /
- metastasis /
- immunohistochemistry

[2] Arpin M,Chirivino D,Naba A,et al.Emerging role for ERM proteins in cell adhesion and igration[J].Cell Adh Migr,2011,5(2):199-206.
[2]	Fehon RG,McClatchey AI,Bretscher A,et al.Organizing the cell cortex:the role of ERM proteins[J].Nat Rev Mol Cell Biol,2010,11(4):276-287.
[3] Zhu J,Feng Y,Ke Z,et al.Down-regulation of miR-183 promotes migration and invasion of osteosarcoma by targeting Ezrin[J].Pathol,2012,180(6):2440-2451.
[4] Briggs JW,Ren L,Nguyen R,et al.The ezrin metastatic phenotype is associated with the initiation of protein translation [J].Neoplasia,2012,14(4):297-310.
[5] Heiska L,Melikova M,Zhao F,et al.Ezrin is key regulator of Srcinduced malignant phenotype in three-dimensional environment[J].Oncogene,2011,30(50):4953-4962.
[6] Janke M,Herrig A,Austermann J,et al.Actin binding of ezrin is activated by specific recognition of PIP2-funetionalized lipid bilayers[J].Biochemistry,2008,47(12):3762-3769.
[7] Meng Y,Lu Z,Yu S,et al.Ezrin promotes invasion and metastasis of pancreatic cancer cells[J].Transl Med,2010,8(1):61-75.
[8] Kang YK,Hong SW,Lee H,et al.Prognostic implications of ezrin expression in human hepatocellular carcinoma[J].Mol Carcinog,2010,49(9):798-804.
[9] Li L,Wang YY,Zhao ZS,et al.Ezrin is associated with gastric cancer progression and prognosis[J].Pathol Oncol Res,2011,17(4):909-915.
[10] Elzagheid A,Korkeila E,Bendardaf R,et al.Intense cytoplasmic ezrin immunoreactivity predicts poor survival in colorectal cancer[J].Hum Pathol,2008,39(12):1737-1743.
[11] Madan R,Brandwein-Gensler M,Elias K,et al.Differential tissue and subcellular expression of ERM proteins in normal and malignant tissues:cytoplasmic ezrin expression has prognostic significance for head and neck squamous cell carcinoma[J].Head Neck,2006,28(11):1018-1027.
[12] Osawa H,Smith CA,Ra YS,et al.The role of the membrane cytoskeleton cross-linker ezrin in medulloblastoma cells [J].Neuro Oncol,2009,11(4):381-393.
[13] Li Q,Wu M,Wang H,et al.Ezrin silencing by small hairpin RNA reverses metastatic behaviors of human breast cancer cells[J].Cancer Lett,2008,261(1):55-63.
[14] Elliott BE,Meens JA,SenGupta SK,et al.The membrane cytoskeleton crosslinker ezrin is required for metastasis of breast carcinoma cells[J].Breast Cancer Res,2005,7(3):365-373.
[15] Mak H,Naba A,Varma S,et al.Ezrin phosphorylation on tyrosine 477 regulates invasion and metastasis of breast cancer cells[J].Cancer,2012,12(1):82-96.
[16] Bruce B,Khanna G,Ren L,et al.Expression of the cytoskeleton linker protein ezrin in human cancers[J].Clin Exp Metastasis,2007,24(2):69-78.
[17] Arslan AA,Silvera D,Arju R,et al.Atypical ezrin localization as a marker of locally advanced breast cancer[J].Breast Cancer Res Treat,2012,134(3):981-988.
[18] Sarrio D,Rodriguez-Pinilla SM,Dotor A,et al.Abnormal ezrin localization is associated with clinicopathological features in invasive breast carcinomas[J].Breast Cancer Res Treat,2006,98 (1):71-79.

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埃兹蛋白在乳腺癌中的表达及意义

作者简介: 王明喜(1973－)，男，硕士，主治医师．

Expression of Ezrin and its significance in breast cancer

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埃兹蛋白在乳腺癌中的表达及意义

作者简介: 王明喜(1973－)，男，硕士，主治医师．

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Expression of Ezrin and its significance in breast cancer

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埃兹蛋白在乳腺癌中的表达及意义

作者简介: 王明喜(1973－)，男，硕士，主治医师．

Expression of Ezrin and its significance in breast cancer

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埃兹蛋白在乳腺癌中的表达及意义

作者简介: 王明喜(1973－)，男，硕士，主治医师．

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Expression of Ezrin and its significance in breast cancer

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