北京市丰台地区呼吸道病毒感染病原学研究
Epidemiological analysis of respiratory virus infection in Fengtai district of Beijing
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摘要: 目的:了解引起北京丰台地区人群急性呼吸道感染的病毒病原。方法:RT-PCR法检测腺病毒、冠状病毒、偏肺病毒;nested-PCR检测呼吸道合胞病毒、副流感病毒(PIV)、鼻病毒、肠道病毒及博卡病毒;Real-time PCR检测甲型流感病毒(IVA)(包括甲1型、甲3型、新甲流型)及乙型流感病毒(IVB)。结果:227份标本中,共有111份标本为病毒阳性,阳性检出率为48. 90%。病毒阳性标本中流感病毒检出率最高,为61. 26%,并以IVB为主,其次为甲3型IVA。PIV位居第二,以PIV1型为主,其次为PIV4,均为聚集性爆发。冬季的病毒检出率较其他季节高,上呼吸道感染患者的病毒检出率较其他患者高。结论:IVB是冬春季引起呼吸道感染的主要病毒病原,冬季是呼吸道病毒感染的高发季节,上呼吸道感染是病毒感染的主要途径。Abstract: Objective: To investigate the virus infected patients with acute respiratory infection in Fengtai district of Beijing. Methods: The adenoviruses，human coronary virus and metapneumovirus were detected using ＲT-PCＲ. The respiratory syncytial virus， parainfluenza virus( PIV) ， rhinovirus， enterovirus and human bocavirus were detected using nested-PCＲ. The influenza A virus( IVA，including A1，A3 and swine flu type) and influenza B virus were detected using Ｒealtime-PCＲ. Results: Among 227 specimens，111specimens with postive virus were detected， the postive detection rate was 48. 90%. The influenza virus detection rate was 61. 26% inpostive virus specimens，which was the highest， IVB was given priority to， followed by influenza A3. The PIV detection rate was the second，PIV1 was given priority to， followed by PIV4， all virus infection were clustering. The positive rate in winter was higher than other seasons， the positive rate in patients with upper respiratory infection was higher than other patients. Conclusions: IVB is the main pathogeny of respiratory infection in 2011 and 2012 winter. Winter is high season of respiratory virus， the main route of virus infection is upper respiratory infection.
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Key words:
- influenza virus /
- parainfluenza virus /
- polymerase chain reaction
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