还原型谷胱甘肽预处理对大鼠小肠淤血性损伤的影响

周玲; 毛立梅; 包蔚雷

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还原型谷胱甘肽预处理对大鼠小肠淤血性损伤的影响

周玲 , 毛立梅 , 包蔚雷

文章导航 > 蚌埠医学院学报 > 2012, 36 > 12: (1418-1420)

引用本文:

Citation:

还原型谷胱甘肽预处理对大鼠小肠淤血性损伤的影响

1.
1. 江苏省靖江市第二人民医院药剂科 214500;
2.
2. 江苏省常州市第二人民医院药剂科;
3.
3. 江苏省靖江市第二人民医院普外科 213003

作者简介: 周玲(1974- ),女,主管药师.

基金项目:

Effects of reduced glutathione precondition on intestine suffering from congestion injury in rats

1.
1. Department of Pharmacy, Jingjiang Second People′s Hospital, Jingjiang Jiangsu 214500;
2.
2. Department of Pharmacy;
3.
3. Department of General Surgery, Changzhou Second People′s Hospital, Changzhou Jiangsu 213003, China

摘要: 目的: 探讨还原型谷胱甘肽(reduced glutathione tablets,GSH)预处理对小肠淤血性损伤的影响。方法: Wistar大鼠分为:淤血组(CG组)、实验组(PG组)和假手术组(SG组)各10只。CG组、PG组阻断门静脉35 min建立小肠淤血性损伤模型;SG组不阻断门静脉。于门静脉阻断前,PG组泵入0.15 g/kg GSH溶液1 ml,CG组和SG组各泵入生理盐水1 ml。各组于建模后24 h分别取门静脉血测定内毒素,心脏取血做丙二醛(MDA)、超氧化物歧化酶(SOD)和肿瘤坏死因子-(TNF-)测定,取末端回肠组织做肠黏膜病理检测。另取10只正常Wistar大鼠的血液及小肠标本作为各组0 h时间点的数值。结果: CG组肠黏膜出现明显损伤,内毒素、MDA及TNF-浓度均显著升高,SOD活性显著降低;与CG组比较,PG组肠黏膜损伤显著减轻,内毒素、MDA及TNF-浓度均显著降低,而SOD活性显著升高(P＜0.01)。结论: GSH预处理对小肠黏膜淤血性损伤可产生显著的保护作用。
- 还原型谷胱甘肽 /
- 小肠 /
- 淤血 /
- 损伤 /
- 大鼠
Abstract: Objective: To study the effects of reduced glutathione(GSH)precondition on intestine suffering from congestion injury in rats.Methods: Wistar rats were divided into 3 groups(n= 10), congestion group(CG),precondition group(PG)and sham operation group(SG).All rats were anesthetized with pentobarbital.One milliliter of GSH(0.15 g /kg)was injected into rats from PG and one milliliter saline was into those from CG and SG.Then all rats underwent laparotomy and those from CG and PG also underwent blockage of port vein for 35 min.After 24 h reperfusion of intestine,blood samples were obtained for the determination of endotoxin,malondialdehyde(MDA), superoxide dismutase(SOD)and tumor necrosis factor-(TNF-); and ileac specimens were for histological examination.Corresponding specimens from normal rats were also obtained as normal controls.Results: All samples and specimens from SG and normal rats were basically normal.But for those from CG, severe injury of intestine was observed combined with significant increasing of endotoxin,MDA,TNF- and remarkable declining of SOD activity.For those from PG,however,moderate intestinal injury was observed and the level of endotoxin,MDA and TNF- were declined significantly, and SOD activity was increased when compared with those parameters in CG(P＜0.01).Conclusions: GSH precondition can produce protective effects on intestine suffering from congestion injury in rats.
- reduced glutathione /
- intestine /
- congestion /
- injury /
- rats

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还原型谷胱甘肽预处理对大鼠小肠淤血性损伤的影响

作者简介: 周玲(1974- ),女,主管药师.

1. 1. 江苏省靖江市第二人民医院药剂科 214500;
2. 2. 江苏省常州市第二人民医院药剂科;
3. 3. 江苏省靖江市第二人民医院普外科 213003

收稿日期: 2011-12-28
网络出版日期: 2012-12-15

基金项目:

关键词:

摘要: 目的: 探讨还原型谷胱甘肽(reduced glutathione tablets,GSH)预处理对小肠淤血性损伤的影响。方法: Wistar大鼠分为:淤血组(CG组)、实验组(PG组)和假手术组(SG组)各10只。CG组、PG组阻断门静脉35 min建立小肠淤血性损伤模型;SG组不阻断门静脉。于门静脉阻断前,PG组泵入0.15 g/kg GSH溶液1 ml,CG组和SG组各泵入生理盐水1 ml。各组于建模后24 h分别取门静脉血测定内毒素,心脏取血做丙二醛(MDA)、超氧化物歧化酶(SOD)和肿瘤坏死因子-(TNF-)测定,取末端回肠组织做肠黏膜病理检测。另取10只正常Wistar大鼠的血液及小肠标本作为各组0 h时间点的数值。结果: CG组肠黏膜出现明显损伤,内毒素、MDA及TNF-浓度均显著升高,SOD活性显著降低;与CG组比较,PG组肠黏膜损伤显著减轻,内毒素、MDA及TNF-浓度均显著降低,而SOD活性显著升高(P＜0.01)。结论: GSH预处理对小肠黏膜淤血性损伤可产生显著的保护作用。

English Abstract

Effects of reduced glutathione precondition on intestine suffering from congestion injury in rats

1. 1. Department of Pharmacy, Jingjiang Second People′s Hospital, Jingjiang Jiangsu 214500;
2. 2. Department of Pharmacy;
3. 3. Department of General Surgery, Changzhou Second People′s Hospital, Changzhou Jiangsu 213003, China

Received Date: 2011-12-28
Available Online: 2012-12-15

Abstract: Objective: To study the effects of reduced glutathione(GSH)precondition on intestine suffering from congestion injury in rats.Methods: Wistar rats were divided into 3 groups(n= 10), congestion group(CG),precondition group(PG)and sham operation group(SG).All rats were anesthetized with pentobarbital.One milliliter of GSH(0.15 g /kg)was injected into rats from PG and one milliliter saline was into those from CG and SG.Then all rats underwent laparotomy and those from CG and PG also underwent blockage of port vein for 35 min.After 24 h reperfusion of intestine,blood samples were obtained for the determination of endotoxin,malondialdehyde(MDA), superoxide dismutase(SOD)and tumor necrosis factor-(TNF-); and ileac specimens were for histological examination.Corresponding specimens from normal rats were also obtained as normal controls.Results: All samples and specimens from SG and normal rats were basically normal.But for those from CG, severe injury of intestine was observed combined with significant increasing of endotoxin,MDA,TNF- and remarkable declining of SOD activity.For those from PG,however,moderate intestinal injury was observed and the level of endotoxin,MDA and TNF- were declined significantly, and SOD activity was increased when compared with those parameters in CG(P＜0.01).Conclusions: GSH precondition can produce protective effects on intestine suffering from congestion injury in rats.