不同菌相白念珠菌对巨噬细胞凋亡的影响

汪春华; 陈艺林

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不同菌相白念珠菌对巨噬细胞凋亡的影响

汪春华 , 陈艺林

文章导航 > 蚌埠医学院学报 > 2013, 37 > 12: (1656-1659)

引用本文:

Citation:

不同菌相白念珠菌对巨噬细胞凋亡的影响

汪春华¹,
陈艺林²

1.
1. 蚌埠医学院第二附属医院检验科, 安徽蚌埠 233040;
2.
2. 蚌埠医学院病原生物学教研室, 安徽蚌埠 233030

作者简介: 汪春华(1976－ ),女,硕士,检验师.

基金项目:

The effect of different flora Candida albicans on the apoptosis of macrophage

WANG Chun-hua¹,
CHEN Yi-lin²

1.
1. Department of Clinical Laboratory, The Second Affiliated Hospital of Bengbu Medical College, Bengbu Anhui 233040;
2.
2. Department of Pathogenic Biology, Bengbu Medical College, Bengbu Anhui 233030, China

摘要: 目的:观察不同菌相白念珠菌诱导巨噬细胞凋亡情况,为疾病的预防和治疗提供思路。方法:用佛波醇酯刺激THP-1细胞分化为巨噬细胞,用酵母相、菌丝相及用胃蛋白酶抑制剂(pepstatin,PEP)预处理的白念珠菌分别作用巨噬细胞1、2、4、8 h;另设空白对照组,AnnexinV-FITC染色后流式细胞仪检测细胞的凋亡率。结果:酵母相、菌丝相、PEP作用组的白念珠菌分别感染巨噬细胞1、2、4、8 h的细胞凋亡率均较空白对照组升高(P0.05~P0.01);各作用组均随时间的延长凋亡率不断升高(P0.01);酵母相组和菌丝相白念珠菌组不同时间细胞凋亡率均较PEP作用组明显增加(P0.01),并且菌丝相组细胞凋亡率明显高于酵母相组(P0.01)。结论:白念珠菌可诱导巨噬细胞凋亡,其作用与其菌相及其分泌型天冬氨酸蛋白酶有关。
- 念珠菌 /
- 巨噬细胞 /
- 菌丝相 /
- 分泌型天冬氨酸蛋白酶 /
- 细胞凋亡
Abstract: Objective: To investigate the apoptosis of macrophage induced by different flora Candida albicans,and provide a new insight for the prevention and treatment of disease. Methods: The THP-1 cells were induced to differentiate into macrophage by phorbol myristate acetate. The macrophage was infected by yeast form,hyphal form of Candida albicans and Candida albicans pre-treated with prepstatin( PEP) for 1 hour,2,4 and 8 hours,the blank control group was also established. The apoptosis rate of macrophage was detected by flow cytometer. Results: Compared with the blank control group,the apoptosis rates of macrophage infected by the yeast form,hyphal form of Candida albicans and Candida albicans pre-treated by PEP for 1 hour,2,4 and 8 hours significantly increased( P 0. 05 to P 0. 01),the apoptosis rate of macrophage increased with the extension of infection time( P 0. 01). Compared with macrophage infected by Candida albicans pre-treated by PEP,the apoptosis rates of macrophage infected by the yeast form and hyphal form of Candida albicans significantly increased in different time-point( P 0. 01). The apoptosis rate of macrophage infected by hyphal form of Candida albicans was significantly higher than that in macrophage infected by yeast form( P 0. 01). Conclusions: Candida albicans can induce the apoptosis of macrophage,the effect of which is related to the form of Candida albicans and secreted aspartic proteinase.
- Candida albicans /
- macrophage /
- hyphal /
- secreted aspartic proteinase /
- apoptosis

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不同菌相白念珠菌对巨噬细胞凋亡的影响

汪春华¹
陈艺林²

作者简介: 汪春华(1976－ ),女,硕士,检验师.

1. 1. 蚌埠医学院第二附属医院检验科, 安徽蚌埠 233040;
2. 2. 蚌埠医学院病原生物学教研室, 安徽蚌埠 233030

收稿日期: 2013-08-15
网络出版日期: 2013-12-15

基金项目:

关键词:

摘要: 目的:观察不同菌相白念珠菌诱导巨噬细胞凋亡情况,为疾病的预防和治疗提供思路。方法:用佛波醇酯刺激THP-1细胞分化为巨噬细胞,用酵母相、菌丝相及用胃蛋白酶抑制剂(pepstatin,PEP)预处理的白念珠菌分别作用巨噬细胞1、2、4、8 h;另设空白对照组,AnnexinV-FITC染色后流式细胞仪检测细胞的凋亡率。结果:酵母相、菌丝相、PEP作用组的白念珠菌分别感染巨噬细胞1、2、4、8 h的细胞凋亡率均较空白对照组升高(P0.05~P0.01);各作用组均随时间的延长凋亡率不断升高(P0.01);酵母相组和菌丝相白念珠菌组不同时间细胞凋亡率均较PEP作用组明显增加(P0.01),并且菌丝相组细胞凋亡率明显高于酵母相组(P0.01)。结论:白念珠菌可诱导巨噬细胞凋亡,其作用与其菌相及其分泌型天冬氨酸蛋白酶有关。

English Abstract

The effect of different flora Candida albicans on the apoptosis of macrophage

WANG Chun-hua¹
CHEN Yi-lin²

1. 1. Department of Clinical Laboratory, The Second Affiliated Hospital of Bengbu Medical College, Bengbu Anhui 233040;
2. 2. Department of Pathogenic Biology, Bengbu Medical College, Bengbu Anhui 233030, China

Received Date: 2013-08-15
Available Online: 2013-12-15

Abstract: Objective: To investigate the apoptosis of macrophage induced by different flora Candida albicans,and provide a new insight for the prevention and treatment of disease. Methods: The THP-1 cells were induced to differentiate into macrophage by phorbol myristate acetate. The macrophage was infected by yeast form,hyphal form of Candida albicans and Candida albicans pre-treated with prepstatin( PEP) for 1 hour,2,4 and 8 hours,the blank control group was also established. The apoptosis rate of macrophage was detected by flow cytometer. Results: Compared with the blank control group,the apoptosis rates of macrophage infected by the yeast form,hyphal form of Candida albicans and Candida albicans pre-treated by PEP for 1 hour,2,4 and 8 hours significantly increased( P 0. 05 to P 0. 01),the apoptosis rate of macrophage increased with the extension of infection time( P 0. 01). Compared with macrophage infected by Candida albicans pre-treated by PEP,the apoptosis rates of macrophage infected by the yeast form and hyphal form of Candida albicans significantly increased in different time-point( P 0. 01). The apoptosis rate of macrophage infected by hyphal form of Candida albicans was significantly higher than that in macrophage infected by yeast form( P 0. 01). Conclusions: Candida albicans can induce the apoptosis of macrophage,the effect of which is related to the form of Candida albicans and secreted aspartic proteinase.