鲍曼不动杆菌108株β-内酰胺酶检测及耐药分析

郭普; 乔艳; 李峰; 郑晶; 沈继龙

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鲍曼不动杆菌108株β-内酰胺酶检测及耐药分析

郭普 , 乔艳 , 李峰 , 郑晶 , 沈继龙

文章导航 > 蚌埠医学院学报 > 2010, 35 > 6: (624-626)

引用本文:

Citation:

鲍曼不动杆菌108株β-内酰胺酶检测及耐药分析

1. 蚌埠医学院第一附属医院检验科, 安徽蚌埠 233004;
2. 安徽医科大学病原生物学教研室安徽省病原生物学省级实验室, 安徽省人兽共患病重点实验室, 安徽合肥 230032;
3. 蚌埠医学院第一附属医院感染病科, 安徽蚌埠 233004

作者简介: 郭普(1976-),男,主管检验师.

通讯作者: 沈继龙, 教授.shenjilong53@126.com

基金项目:
蚌埠医学院课题基金资助项目(07024)
中图分类号: R378

Detection and drug resistance of β-lactamase in 108 strains of Acinetobacter baumanii

1. Department of Clinical Laboratory, The First Affiliated Hospital of Bengbu Medical College, Bengbu Anhui 233004;
2. Department of Microbiology and Parasitology, Province Laboratory of Pathogen Biology, and Anhui Key Laboratory of Zoonoses, Anhui Medical University, Hefei Anhui 230032, China
3. Department of Infection, The First Affiliated Hospital of Bengbu Medical College, Bengbu Anhui 233004;

Corresponding author: SHEN Ji-long, 教授.shenjilong53@126.com

CLC number: R378

摘要: 目的:分析鲍曼不动杆菌的医院感染分布特点、产β-内酰胺酶情况和耐药性。方法:对临床送检标本中分离出的108株引起医院感染的鲍曼不动杆菌分布及药敏结果进行回顾性分析,并分别采用硝基头孢噻吩纸片法、双纸片协同法、三维试验检测β-内酰胺酶、超广谱β-内酰胺酶(ESBLs)和头孢菌素酶。结果:鲍曼不动杆菌在重症监护治疗病房、神经外科的检出率分别为63.89%、11.11%;感染部位以呼吸道和手术切口为主,分别为77.78%和12.96%。该菌对临床常用抗生素有不同程度的耐药,发现多重耐药菌96株(88.89%)。108株鲍曼不动杆菌中共检出产ESBLs 11株,阳性率为10.19%;头孢菌素酶78株,阳性率为72.22%;产β-内酰胺酶阳性率为100.00%。结论:鲍曼不动杆菌是医院感染重要的条件致病菌,其对抗生素耐药率高,且多重耐药。产β-内酰胺酶为鲍曼不动杆菌耐药的主要原因之一。临床要重视合理使用抗生素,减少多重耐药菌株的产生。
- 鲍曼不动杆菌 /
- β-内酰胺酶 /
- 耐药
Abstract: Objective:To investigate the clinical distribution,production and drug resistance of β-lactamase in nosocomial infection.Methods:The distribution and the drug resistance of 108 strains of Acinetobacter baumanii causing nosocomial infection were analyzed retrospectively.β-lactamase,cephalosporinase and extended spectrum β-lactamases (ESBLs) were detected by Nitrocefin test,3-dimension test and disc agar diffusion method,respectively.Results:The detection rates of Acinetobacter baumanii in ICU wards and neurosurgery wards were 63.89% and 11.11%,respectively.Acinetobacter baumanii was detected in 77.78% of the sputum samples and 12.96% of the secretion samples.The bacteria were resistant to most common antibiotics,and the rate of multiple-resistance was 88.89%.Eleven ESBLs bacteria and 78 cephalosporinase bacteria were detected in the 108 strains of Acinetobacter baumanii,accounting for 10.19% and 72.22%,respectively,and the positive rate of β-lactamase bacteria detected was 100.00%.Conclusions:Acinetobacter baumannii are the major opportunistic pathogenic bacteria in hospital infection.They are highly resistant to routine antimicrobial agents and even multi-drug resistant.The strains producing β-lactamase are the prominent cause of multi-drug resistance.Antimicrobial agents should be used rationally to decrease multi-drug resistance.
- Acinetobacter baumanii /
- β-lactamase /
- antibiotic resistance

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鲍曼不动杆菌108株β-内酰胺酶检测及耐药分析

通讯作者: 沈继龙, 教授.shenjilong53@126.com

作者简介: 郭普(1976-),男,主管检验师.

1. 蚌埠医学院第一附属医院检验科, 安徽蚌埠 233004;
2. 安徽医科大学病原生物学教研室安徽省病原生物学省级实验室, 安徽省人兽共患病重点实验室, 安徽合肥 230032;
3. 蚌埠医学院第一附属医院感染病科, 安徽蚌埠 233004

收稿日期: 2009-06-22

基金项目: 蚌埠医学院课题基金资助项目(07024)

关键词:

摘要: 目的:分析鲍曼不动杆菌的医院感染分布特点、产β-内酰胺酶情况和耐药性。方法:对临床送检标本中分离出的108株引起医院感染的鲍曼不动杆菌分布及药敏结果进行回顾性分析,并分别采用硝基头孢噻吩纸片法、双纸片协同法、三维试验检测β-内酰胺酶、超广谱β-内酰胺酶(ESBLs)和头孢菌素酶。结果:鲍曼不动杆菌在重症监护治疗病房、神经外科的检出率分别为63.89%、11.11%;感染部位以呼吸道和手术切口为主,分别为77.78%和12.96%。该菌对临床常用抗生素有不同程度的耐药,发现多重耐药菌96株(88.89%)。108株鲍曼不动杆菌中共检出产ESBLs 11株,阳性率为10.19%;头孢菌素酶78株,阳性率为72.22%;产β-内酰胺酶阳性率为100.00%。结论:鲍曼不动杆菌是医院感染重要的条件致病菌,其对抗生素耐药率高,且多重耐药。产β-内酰胺酶为鲍曼不动杆菌耐药的主要原因之一。临床要重视合理使用抗生素,减少多重耐药菌株的产生。

English Abstract

Detection and drug resistance of β-lactamase in 108 strains of Acinetobacter baumanii

Corresponding author: SHEN Ji-long, 教授.shenjilong53@126.com

1. Department of Clinical Laboratory, The First Affiliated Hospital of Bengbu Medical College, Bengbu Anhui 233004;
2. Department of Microbiology and Parasitology, Province Laboratory of Pathogen Biology, and Anhui Key Laboratory of Zoonoses, Anhui Medical University, Hefei Anhui 230032, China
3. Department of Infection, The First Affiliated Hospital of Bengbu Medical College, Bengbu Anhui 233004;

Received Date: 2009-06-22

Abstract: Objective:To investigate the clinical distribution,production and drug resistance of β-lactamase in nosocomial infection.Methods:The distribution and the drug resistance of 108 strains of Acinetobacter baumanii causing nosocomial infection were analyzed retrospectively.β-lactamase,cephalosporinase and extended spectrum β-lactamases (ESBLs) were detected by Nitrocefin test,3-dimension test and disc agar diffusion method,respectively.Results:The detection rates of Acinetobacter baumanii in ICU wards and neurosurgery wards were 63.89% and 11.11%,respectively.Acinetobacter baumanii was detected in 77.78% of the sputum samples and 12.96% of the secretion samples.The bacteria were resistant to most common antibiotics,and the rate of multiple-resistance was 88.89%.Eleven ESBLs bacteria and 78 cephalosporinase bacteria were detected in the 108 strains of Acinetobacter baumanii,accounting for 10.19% and 72.22%,respectively,and the positive rate of β-lactamase bacteria detected was 100.00%.Conclusions:Acinetobacter baumannii are the major opportunistic pathogenic bacteria in hospital infection.They are highly resistant to routine antimicrobial agents and even multi-drug resistant.The strains producing β-lactamase are the prominent cause of multi-drug resistance.Antimicrobial agents should be used rationally to decrease multi-drug resistance.