来源于HHV8 MIP N端多肽抗乳腺癌作用的实验研究

李成华; 丁勇兴; 杨清玲; 陈昌杰; 李馨

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

来源于HHV8 MIP N端多肽抗乳腺癌作用的实验研究

李成华 , 丁勇兴 , 杨清玲 , 陈昌杰 , 李馨

文章导航 > 蚌埠医学院学报 > 2009, 34 > 6: (464-467)

引用本文:

Citation:

来源于HHV8 MIP N端多肽抗乳腺癌作用的实验研究

1. 安徽省蚌埠市第三人民医院肿瘤外科, 233000;
2. 蚌埠医学院临床检验诊断实验中心, 安徽蚌埠 233030;
3. 华中科技大学生物技术系, 湖北武汉 430074

作者简介: 李成华(1960-),男,主任医师.

基金项目:
安徽省蚌埠市科技计划项目(蚌科200617号)

安徽省自然科学基金资助项目(070413119)

安徽省临床医学应用技术研究计划项目(068105)
中图分类号: R737.9

Role of NT21MP from HHV8 MIP N-terminal peptide in resisting breast cancer

1. Department of Oncology, Bengbu Third People's Hospital, Bengbu Anhui 233000;
2. Clinical Testing and Diagnose Experimental Center, Bangbu Medical College, Bengbu Anhui 233030;
3. Department of Biotechnology, Huazhong University of Science and Technology, Wuhan Ilubei 430074, China
CLC number: R737.9

摘要: 目的:探讨来源于HHV8 MIP N端多肽(NT21MP)治疗小鼠乳腺癌的疗效。方法:采用乳腺癌细胞株4T-1构建乳腺癌小鼠模型;实验分为NT21MP5μg/kg、50μg/kg和500μg/kg组,NT21MP与Herceptin联合用约组(NT21MP50μg/kg+Herceptin 36.04μg/kg)、阳性对照组(Herceptin 36.04μg/kg)及生理盐水对照组;观察各组小鼠肿瘤体积大小,并计算抑瘤率;检测肺转移结节。结果:与生理盐水组荷瘤小鼠比较,NT21MP呈剂量依赖性地抑制肿瘤的生长,以联合用药组为明显(P<0.01)。NT21MP不同浓度、联合用药及阳性对照组的抑瘤率分别为10.0%、41.6%、81.0%、58.2%及39.2%;对照组小鼠肺内均见转移性Lewis肺癌瘤灶,肺脏表面可见多个肿瘤转移结节;NT21MP 5μg/kg组小鼠肺内均见转移性Lewis肺癌瘤灶,肺脏表面可见到散在的肿瘤转移结节;NT21MP 50μg/kg组和Herceptin组中,各有5/6动物肺内见转移性Lewis肺癌瘤灶,肺表面可见小的单个肿瘤转移结节;NT21MP 500μg/kg组和联合用药组各有2/6和3/6动物肺内见转移性Lewis肺癌瘤灶,肺表面未见明显的转移结节。结论:NT21MP可抑制乳腺癌的生长和转移,联合应用基因靶向药物Herceptin,可提高对乳腺癌靶向治疗的效果。
- 乳腺肿瘤,实验性 /
- 人疱疹病毒 /
- NT21MP /
- 肺转移 /
- 小鼠,近交系BALB/c
Abstract: Objective: To investigate the function of NT21MP from human herpesvirus-8(HHV8) monocyte inflammatory protein(MIP) N-terminal peptide in resisting breast cancer.Methods: Breast carcinoma model mice were established by 4T-1 breast cancer cell strains.The models were divided into NT21 MP group(5μg/kg,50μg/kg and 500 μg/kg),combination therapy group(NT21MP 50 μg/kg + Herceptin 36.04 μg/kg).positive control group(Herceptin 36.04 μg/kg) and sodium chloride group.The size of the tumor on the tumor-bearing mice was observed,the tumor inhibitory rate was calculated and the metastasis lung node was detected.Results: Compared with the tumor-bearing mice in sodium chloride group,NT21 MP inhibited the growth of tumor in a dose-dependent manner,which was the most evident in the combination group(P < 0.01).The tumor inhibitory rates in the NT21MP groups with different concentrations,the combination group and the positive control group were 10.0%,41 6%,81.0%,58.2% and 39.2% respectively.Metastasis Lewis lung cancers were observed in both of the control groups,and several metastasis nodes of white tumor were observed on the surface of the mice's lung;and the situation was the same with NT21MP 5 μ/kg group.In NT21 MP 50 μg/kg group and Herceptin group,metastasis Lewis lung cancers were noted in the lungs of five sixths of the mice,and small and single metastasis node was found on the surface of the lung.In the NT21 MP 500 μg/kg group and the combination therapy group,metastasis Lewis lung cancers were noted in two sixths and three sixths of the mice,respectively,and no obvious metastasis nodes were found on the surface of the mice's lung.Conclusions: NT21MP may inhibit the growth and metastasis of breast cancer.Combination therapy of NT21 MP and the targeted drug Herceptin can improve the clinical efficacy.
- breast neoplams,experimental /
- herpesvirus hominis /
- NT2IMP /
- pulmonary metastasis /
- mice,inbred BALB/c

[1]	胡永全 , 申勇 , 袁超 , 陶新全 , 刘恒超 , 李卫鹏 . ¹³¹Ⅰ治疗分化型甲状腺癌肺转移的疗效及影响因素分析. 蚌埠医学院学报, 2019, 44(1): 34-36, 40. doi: 10.13898/j.cnki.issn.1000-2200.2019.01.010
[2]	时凤宝 , 卞巍 . 右小腿高级别恶性肌纤维母细胞肉瘤伴肺部转移瘤1例. 蚌埠医学院学报, 2014, 39(10): 1456-1457.
[3]	左联 , 谢鹏 , 徐霞红 , 胡晖 , 顾晓波 , 黄东雅 . Balb/c小鼠单纯疱疹病毒性脑炎模型的建立. 蚌埠医学院学报, 2013, 37(2): 131-133;130.
[4]	赵武 . 小儿单纯疱疹病毒脑炎22例临床分析. 蚌埠医学院学报, 2004, 29(5): 395-397.
[5]	赵同清 , 杨阳 , 王元元 , 陶明飞 . 利脑心胶囊对小鼠实验性缺氧的保护作用. 蚌埠医学院学报, 2017, 42(2): 145-147. doi: 10.13898/j.cnki.issn.1000-2200.2017.02.002
[6]	吴正中 , 吴俊英 , 王芳 , 李见 , 江金群 , 张坡 . Pristane诱导BALB/c小鼠系统性红斑狼疮模型的建立. 蚌埠医学院学报, 2013, 37(5): 516-523.
[7]	郑中显 , 陈倩 , 侍伟伟 , 鲍瑜 , 潘明 . 分次立体定向放射治疗肺转移瘤31例疗效和安全性分析. 蚌埠医学院学报, 2013, 37(4): 407-409.
[8]	顾蓉蓉 , 丁长伟 . miRNA-200c/SOX2轴在实验性牙周炎牙槽骨吸收中的作用研究. 蚌埠医学院学报, 2023, 48(3): 281-285. doi: 10.13898/j.cnki.issn.1000-2200.2023.03.001
[9]	刘超 , 宋玮 , 徐凤珍 . N-乙酰半胱氨酸对小鼠肺腺癌生长、转移的抑制作用. 蚌埠医学院学报, 2005, 30(3): 218-220.
[10]	马锐 , 徐欢 , 杨丽丽 , 程亮 , 唐云国 , 叶云 . 刺五加注射液对小鼠实验性急性心肌缺氧的保护作用. 蚌埠医学院学报, 2006, 31(1): 17-19.
[11]	芮艳 , 胡丹凤 , 黄礼年 . 达肝素钠抑制人肺腺癌细胞增殖作用的实验观察. 蚌埠医学院学报, 2014, 38(3): 284-287.
[12]	成红 , 夏丹 , 唐婷婷 , 沙泉 . 实验性矽肺模型大鼠血清TGF-1和TNF-的变化. 蚌埠医学院学报, 2012, 36(4): 386-388.
[13]	陶新全 , 袁超 , 刘恒超 , 周翔 . 氯化锶治疗转移性骨肿瘤疗效评价. 蚌埠医学院学报, 2005, 30(3): 241-243.
[14]	年峰 , 彭开桂 . 乳腺癌转移抑制基因在肿瘤中的研究进展. 蚌埠医学院学报, 2010, 35(3): 318-321.
[15]	吴少帅 , 徐福林 , 苏作鹏 , 杜嘉瑞 , 沈刚 . 芝麻素对Toll样受体2在实验性颅脑损伤中表达的影响. 蚌埠医学院学报, 2020, 45(1): 23-25. doi: 10.13898/j.cnki.issn.1000-2200.2020.01.006
[16]	刘戈 , 唐震 , 刘学刚 , 施超 , 王祖义 , 刘以尧 , 段贵新 . 肺鳞状细胞癌251例淋巴结转移规律分析. 蚌埠医学院学报, 2015, 40(2): 155-158. doi: 10.13898/j.cnki.issn.1000-2200.2015.02.005
[17]	杨庆宇 , 梁冰 , 周海艳 , 潘攀 . 莫西沙星治疗老年人慢性阻塞性肺疾病急性发作的疗效. 蚌埠医学院学报, 2013, 37(6): 663-665.
[18]	贾伟 , 王伟 , 胡冰 . 转移性乳腺癌化疗后低钠血症致意识障碍1例. 蚌埠医学院学报, 2017, 42(4): 554-555. doi: 10.13898/j.cnki.issn.1000-2200.2017.04.041
[19]	马进 , 高明 . 猪肺表面活性物质治疗新生儿肺透明膜病21例疗效观察. 蚌埠医学院学报, 2009, 34(1): 43-45.
[20]	王娇 , 邹琪 , 刘成 , 楚新旭 , 汪华学 . 维生素C预处理对脓毒症小鼠肺组织miR126a-5p、miR155-5p表达的影响. 蚌埠医学院学报, 2022, 47(4): 433-437. doi: 10.13898/j.cnki.issn.1000-2200.2022.04.003

点击查看大图

计量

文章访问数: 3836
HTML全文浏览量: 381
PDF下载量: 4
被引次数: 0

来源于HHV8 MIP N端多肽抗乳腺癌作用的实验研究

作者简介: 李成华(1960-),男,主任医师.

1. 安徽省蚌埠市第三人民医院肿瘤外科, 233000;
2. 蚌埠医学院临床检验诊断实验中心, 安徽蚌埠 233030;
3. 华中科技大学生物技术系, 湖北武汉 430074

收稿日期: 2008-08-29

基金项目: 安徽省蚌埠市科技计划项目(蚌科200617号)安徽省自然科学基金资助项目(070413119)安徽省临床医学应用技术研究计划项目(068105)

关键词:

摘要: 目的:探讨来源于HHV8 MIP N端多肽(NT21MP)治疗小鼠乳腺癌的疗效。方法:采用乳腺癌细胞株4T-1构建乳腺癌小鼠模型;实验分为NT21MP5μg/kg、50μg/kg和500μg/kg组,NT21MP与Herceptin联合用约组(NT21MP50μg/kg+Herceptin 36.04μg/kg)、阳性对照组(Herceptin 36.04μg/kg)及生理盐水对照组;观察各组小鼠肿瘤体积大小,并计算抑瘤率;检测肺转移结节。结果:与生理盐水组荷瘤小鼠比较,NT21MP呈剂量依赖性地抑制肿瘤的生长,以联合用药组为明显(P<0.01)。NT21MP不同浓度、联合用药及阳性对照组的抑瘤率分别为10.0%、41.6%、81.0%、58.2%及39.2%;对照组小鼠肺内均见转移性Lewis肺癌瘤灶,肺脏表面可见多个肿瘤转移结节;NT21MP 5μg/kg组小鼠肺内均见转移性Lewis肺癌瘤灶,肺脏表面可见到散在的肿瘤转移结节;NT21MP 50μg/kg组和Herceptin组中,各有5/6动物肺内见转移性Lewis肺癌瘤灶,肺表面可见小的单个肿瘤转移结节;NT21MP 500μg/kg组和联合用药组各有2/6和3/6动物肺内见转移性Lewis肺癌瘤灶,肺表面未见明显的转移结节。结论:NT21MP可抑制乳腺癌的生长和转移,联合应用基因靶向药物Herceptin,可提高对乳腺癌靶向治疗的效果。

English Abstract

Role of NT21MP from HHV8 MIP N-terminal peptide in resisting breast cancer

1. Department of Oncology, Bengbu Third People's Hospital, Bengbu Anhui 233000;
2. Clinical Testing and Diagnose Experimental Center, Bangbu Medical College, Bengbu Anhui 233030;
3. Department of Biotechnology, Huazhong University of Science and Technology, Wuhan Ilubei 430074, China

Received Date: 2008-08-29

Abstract: Objective: To investigate the function of NT21MP from human herpesvirus-8(HHV8) monocyte inflammatory protein(MIP) N-terminal peptide in resisting breast cancer.Methods: Breast carcinoma model mice were established by 4T-1 breast cancer cell strains.The models were divided into NT21 MP group(5μg/kg,50μg/kg and 500 μg/kg),combination therapy group(NT21MP 50 μg/kg + Herceptin 36.04 μg/kg).positive control group(Herceptin 36.04 μg/kg) and sodium chloride group.The size of the tumor on the tumor-bearing mice was observed,the tumor inhibitory rate was calculated and the metastasis lung node was detected.Results: Compared with the tumor-bearing mice in sodium chloride group,NT21 MP inhibited the growth of tumor in a dose-dependent manner,which was the most evident in the combination group(P < 0.01).The tumor inhibitory rates in the NT21MP groups with different concentrations,the combination group and the positive control group were 10.0%,41 6%,81.0%,58.2% and 39.2% respectively.Metastasis Lewis lung cancers were observed in both of the control groups,and several metastasis nodes of white tumor were observed on the surface of the mice's lung;and the situation was the same with NT21MP 5 μ/kg group.In NT21 MP 50 μg/kg group and Herceptin group,metastasis Lewis lung cancers were noted in the lungs of five sixths of the mice,and small and single metastasis node was found on the surface of the lung.In the NT21 MP 500 μg/kg group and the combination therapy group,metastasis Lewis lung cancers were noted in two sixths and three sixths of the mice,respectively,and no obvious metastasis nodes were found on the surface of the mice's lung.Conclusions: NT21MP may inhibit the growth and metastasis of breast cancer.Combination therapy of NT21 MP and the targeted drug Herceptin can improve the clinical efficacy.