还原型谷胱甘肽对糖尿病大鼠肾小球内皮功能的影响

刘滇军; 刘辉辉; 沈建明; 田少江; 王黎萍; 李骏峰

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还原型谷胱甘肽对糖尿病大鼠肾小球内皮功能的影响

刘滇军 , 刘辉辉 , 沈建明 , 田少江 , 王黎萍 , 李骏峰

文章导航 > 蚌埠医学院学报 > 2014, 39 > 11: (1464-1466)

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还原型谷胱甘肽对糖尿病大鼠肾小球内皮功能的影响

湖北医药学院附属人民医院肾内科, 湖北十堰 442000

作者简介: 刘滇军(1981-),男,主治医师.

中图分类号: R692.39

The effect of reduced glutathione on glomerular endothelial function in diabetic rats

Department of Nephrology, Renmin Hospital, Huibei University of Medicine, Shiyan Hubei 442000, China
CLC number: R692.39

摘要: 目的:观察早期糖尿病肾病(diabetic nephropathy,DN)大鼠肾小球内皮功能的特点,探讨还原型谷胱甘肽对早期DN大鼠内皮功能的影响。方法:将大鼠分为对照组、模型组和治疗组,应用链脲佐菌素腹腔注射建立DN模型,治疗组每天腹腔注射还原型谷胱甘肽。应用相关试剂盒检测尿白蛋白(UA)、肾小球组织一氧化氮(NO)、内皮型一氧化氮合酶(eNOS)活性、血管性假性血友病因子(vWF)和超氧化物歧化酶、丙二醛(MDA)、谷胱甘肽过氧化物酶。结果:与对照组比较,模型组早期DN大鼠肾小球NO含量、eNOS活性均显著下降,vWF水平明显升高,UA显著增加(P<0.01),且UA增加的程度与vWF升高的水平呈正相关关系(P<0.05);肾小球组织中超氧化物歧化酶、谷胱甘肽过氧化物酶均明显下降,MDA明显升高(P<0.01)。与模型组比较,治疗组大鼠肾小球NO含量、eNOS活性均明显上升,vWF水平显著降低,UA明显减少,肾小球组织中MDA显著下降(P<0.01)。结论:早期DN大鼠肾小球内皮功能紊乱,氧化应激水平明显升高,还原型谷胱甘肽可能通过降低早期DN大鼠肾小球内氧化应激水平从而改善肾小球内皮功能。
- 糖尿病肾病 /
- 内皮功能紊乱 /
- 还原型谷胱甘肽 /
- 氧化应激
Abstract: Objective: To observe the feature of glomerular endothelial function in rats with early diabetic nephropathy(DN),and evaluate the effect of reduced glutathione on glomerular endothelial function in diabetic rats.Methods: Streptozotocin(60mg/ kg) was injected into peritoneal cavity in the rats to establish animal DN model.The diabetic rats were randomly divided into DN model group and reduced glutathione treated group.Coherent kits were used to detect glomerular nitrogen monoxide(NO),the activity of glomerular endothelial nitric oxide synthase(eNOS),von Willebrand factor(vWF),urinary albumin(UA),superoxide dismutase(SOD),malondialdehyde(MDA) and glutathione peroxidase.Results: Compared with normal control group,glomerular NO,activities of glomerular eNOS were significantly decreased in early DN rats,while the level of vWF and UA significantly increased(P<0.01),and the level of UA was correlated with the level of glomerular vWF(P<0.05).Glomerular SOD,glutathione peroxidase significantly decreased in early DN rats,while MDA was significantly increased(P<0.01).Compared with DN model group,glomerular NO,activities of glomerular eNOS were significantly increased in early DN rats treated with reduced glutathione,while the level of vWF and UA were significantly decreased,and glomerular MDA was significantly increased in early DN rats treated with reduced glutathione(P<0.01).Conclusions: Glomerular endothelial function is indiscriminate in early DN rats,while the level of oxidative stress significantly increases.Reduced glutathione improves glomerular endothelial dysfunction in early DN rats,maybe through reducing oxidative stress.
- diabetic nephropathy /
- endothelial dysfunction /
- reduced glutathione /
- oxidative stress

[1]	Schmieder RE.Endothelial dysfunction:how can one intervene at the beginning of the cardiovascular continuum?[J].J Hypertens Suppl,2006,24(2):S31-S35.
[2]	胡欣,张弘,郝丽莉,等.还原型谷胱甘肽对2型糖尿病内皮功能的影响[J].中国误诊学杂志,2008,8(25):6108-6109.
[3]	Tesch GH,Lim AK.Recent insights into diabetic renal injury from the db/db mouse model of type 2 diabetic nephropathy[J].Am J Physiol Renal Physiol,2011,300(2):F301-F310.
[4]	Bourque SL,Davidge ST,Adams MA.The interaction between endothelin-1 and nitric oxide in the vasculature:new perspectives[J].Am J Physiol Regul Integr Comp Physiol,2011,300(6):R1288-R1295.
[5]	Crabtree MJ,Hale AB,Channon KM.Dihydrofolate reductase protects endothelial nitric oxide synthase from uncoupling in tetrahydrobiopterin deficiency[J].Free Radic Biol Med,2011,50(11):1639-1646.
[6]	Adel A,Abdel-Salam Z,Nammas W.Low-Dose statin therapy improves endothelial function in type 2 diabetic patients with normal serum total cholesterol:a randomized placebo-controlled study[J].J Clin Hypertens,2010,12(10):820-825.
[7]	Dendorfer A,Dominiak P,Schunkert H.ACE inhibitors and angiotensin Ⅱ receptor antagonists[J].Handb Exp Pharmacol,2005,170(1):407-442.
[8]	Lu X,Guo X,Karathanasis SK,et al.Rosiglitazone reverses endothelial dysfunction but not remodeling of femoral artery in Zucker diabetic fatty rats[J].Cardiovasc Diabetol,2010,9(1):19-26.
[9]	Cheng ZJ,Gronholm T,Louhelainen M,et al.Vascular and renal effects of vasopeptidase inhibition and angiotensin-converting enzyme blockade in spontaneously diabetic Goto-Kakizaki rats[J].J Hypertens,2005,23(9):1757-1770.

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还原型谷胱甘肽对糖尿病大鼠肾小球内皮功能的影响

作者简介: 刘滇军(1981-),男,主治医师.

湖北医药学院附属人民医院肾内科, 湖北十堰 442000

收稿日期: 2013-11-18

关键词:

摘要: 目的:观察早期糖尿病肾病(diabetic nephropathy,DN)大鼠肾小球内皮功能的特点,探讨还原型谷胱甘肽对早期DN大鼠内皮功能的影响。方法:将大鼠分为对照组、模型组和治疗组,应用链脲佐菌素腹腔注射建立DN模型,治疗组每天腹腔注射还原型谷胱甘肽。应用相关试剂盒检测尿白蛋白(UA)、肾小球组织一氧化氮(NO)、内皮型一氧化氮合酶(eNOS)活性、血管性假性血友病因子(vWF)和超氧化物歧化酶、丙二醛(MDA)、谷胱甘肽过氧化物酶。结果:与对照组比较,模型组早期DN大鼠肾小球NO含量、eNOS活性均显著下降,vWF水平明显升高,UA显著增加(P<0.01),且UA增加的程度与vWF升高的水平呈正相关关系(P<0.05);肾小球组织中超氧化物歧化酶、谷胱甘肽过氧化物酶均明显下降,MDA明显升高(P<0.01)。与模型组比较,治疗组大鼠肾小球NO含量、eNOS活性均明显上升,vWF水平显著降低,UA明显减少,肾小球组织中MDA显著下降(P<0.01)。结论:早期DN大鼠肾小球内皮功能紊乱,氧化应激水平明显升高,还原型谷胱甘肽可能通过降低早期DN大鼠肾小球内氧化应激水平从而改善肾小球内皮功能。

English Abstract

The effect of reduced glutathione on glomerular endothelial function in diabetic rats

Department of Nephrology, Renmin Hospital, Huibei University of Medicine, Shiyan Hubei 442000, China

Received Date: 2013-11-18

Abstract: Objective: To observe the feature of glomerular endothelial function in rats with early diabetic nephropathy(DN),and evaluate the effect of reduced glutathione on glomerular endothelial function in diabetic rats.Methods: Streptozotocin(60mg/ kg) was injected into peritoneal cavity in the rats to establish animal DN model.The diabetic rats were randomly divided into DN model group and reduced glutathione treated group.Coherent kits were used to detect glomerular nitrogen monoxide(NO),the activity of glomerular endothelial nitric oxide synthase(eNOS),von Willebrand factor(vWF),urinary albumin(UA),superoxide dismutase(SOD),malondialdehyde(MDA) and glutathione peroxidase.Results: Compared with normal control group,glomerular NO,activities of glomerular eNOS were significantly decreased in early DN rats,while the level of vWF and UA significantly increased(P<0.01),and the level of UA was correlated with the level of glomerular vWF(P<0.05).Glomerular SOD,glutathione peroxidase significantly decreased in early DN rats,while MDA was significantly increased(P<0.01).Compared with DN model group,glomerular NO,activities of glomerular eNOS were significantly increased in early DN rats treated with reduced glutathione,while the level of vWF and UA were significantly decreased,and glomerular MDA was significantly increased in early DN rats treated with reduced glutathione(P<0.01).Conclusions: Glomerular endothelial function is indiscriminate in early DN rats,while the level of oxidative stress significantly increases.Reduced glutathione improves glomerular endothelial dysfunction in early DN rats,maybe through reducing oxidative stress.