Abstract: Objective: To investigate the pathogenic bacterial distribution and drug resistance in urinary system infection so as to provide basis for clinic selection of antibiotics.Methods: A total of 213 strains of pathogenic bacterium of urinary system infection were identified,for which Kirly-Bauer test was used for drug sensitivity test.Extended spectrum β lactamases (ESBLs)-producing test was applied in the detection of Escherichia coli and Klebsiella pneumoniae.Results: One hundred and fifteen strains of Gram-negative bacillus,52 strains of fungus and 46 strains of Gram-positive coccus were detected.The first five species in order were Escherichia coli (n=69),fungus (n=52),Enterococcus (n=30),Klebsiella pneumoniae (n=15)and staphylococcus (n=11).The detection rates of ESBLs-producing Escherichia coli and ESBLs-producing Klebsiella pneumoniae were 50.7% and 60.0% respectively.The test of drug sensitivity in the main pathogens except fungus indicated that in Gram-negative bacillus,the drug resistance to imipenem was the lowest and Gram-positive coccus also had a serious drug resistance status,but all of them were sensitive to vancomycin and teicoplanin.Conclusions: Escherichia coli remain the most frequent pathogenic bacteria in urinary system infection.The rate of fungus infection tends to increase,and drug resistance is serious.Clinicians should pay more attention to the result of urine culture and select antibiotics according to the drug sensitive test.