解脲脲原体感染对大鼠睾丸组织结构及睾酮水平的影响
Influence of ureaplasma urealyticum infection on the structure of testicular tissue and the level of testosterone in rats
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摘要: 目的:探讨解脲脲原体(ureaplasma urealyticum,UU)感染对大鼠睾丸组织结构及睾酮水平的影响.方法:清洁级SD大鼠40只随机分为实验组A(术后7 d组,A组)、实验组B(术后14 d组,B组),对照组C(术后7 d组,C组)和对照组D(术后14 d组,D组),每组10只.A、B组膀胱注射0.6 ml UU4型菌株(1×107 CCU/ml),C、D组膀胱注射等体积的UU液体培养基.术后第7天取A和C组、第14天取B和D组大鼠睾丸组织HE染色观察结构,相应时间取4组大鼠睾丸间质液和血浆,采用化学发光法检测睾酮水平.结果:HE染色显示A、B组呈炎症性病理变化,曲细精管生精细胞层次和数量减少,基底膜破坏,炎细胞浸润,成熟精子减少,但B组炎症病变较A组轻,C、D组睾丸结构正常.A、B组血浆睾酮水平均明显低于C、D组(P<0.01),但B组与A组血浆睾酮水平差异无统计学意义(P>0.05).A组睾丸间质液睾酮水平均明显低于B、C和D组(P<0.01),而B、C和D组睾丸间质液睾酮水平差异均无统计学意义(P>0.05).结论:大鼠感染UU后睾丸组织可出现病理性改变,睾酮分泌功能降低,随时间的延长,睾丸组织结构及睾酮水平逐渐恢复.Abstract: Objective:To study the influences of ureaplasma urealyticum(UU) infection on the structure of testicular tissue and level of testosterone in rats.Methods:Forty clean grade male Sprague dawley(SD) rats were randomly divided into the experiment group A(at 7 d after surgery), experiment group B(at 14 d after surgery, group B), control group C(at 7 d after surgery) and control group D(at 14 d after surgery)10 rats each group.The group A and B were injected with 0.6 ml UU4(1×107CCU/ml) through punctio vesicae, and the group C and D were injected with the same volume of UU liquid medium.At day 7 and 14 after injection, the structures of testicular tissue of all rats were observed by HE staining, the levels of testosterone in the testis interstitial fluid and plasma of all rats were detected by chemiluminescence method.Results:The changes of inflammatory pathology(including the layer and amount of seminiferous tubule spermatogenic cell decreasing, basement membrane lesion, inflammatory cell infiltrating and mature sperms decreasing) in the testis of group A and B were found by HE staining, the inflammatory changes in group B were lighter than those in group A.The structures of testicular tissue in group C and D were normal.The levels of plasma testosterone in group A and B were significantly lower than that in group C and D(P<0.01), the difference of plasma testosterone level between group A and B was not statistically significant(P>0.05).The testosterone level in testis interstitial fluid in group A was significantly lower than that in group B, C and D(P<0.01), the differences of the testosterone levels in testis interstitial fluid between group B, C and D were not statistical significant(P>0.05).Conclusions:UU infection can lead to the pathologic change of testicular tissue and testosterone secretion decrease.With the extension of time, the testicular tissue structure and testosterone level can gradually recover.
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Key words:
- ureaplasma urealyticum /
- infection /
- testis /
- pathological lesion /
- testosterone /
- rat
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