刺五加注射液对小鼠急性酒精性肝损伤的保护作用
Protective effect of Acanthopanax senticosus injection on the acute alcoholic hepatic injury in mice
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摘要: 目的:观察刺五加注射液对小鼠酒精性肝损伤的保护作用。方法:昆明种雄性小鼠60只(18～22 g),随机分为正常组、模型组、硫普罗宁阳性药对照组和刺五加高、中、低剂量组(200 mg/kg、100 mg/kg、50 mg/kg),每组各10只。采用50%乙醇12 ml·kg-1·d-1连续灌胃10 d,制备小鼠酒精性肝损伤模型。测定血清中天门冬氨酸氨基转移酶、丙氨酸氨基转移酶及三酰甘油(TG)水平;肝组织丙二醛、还原型谷胱甘肽含量,计算肝脏指数,并观察肝脏病理变化。结果:小鼠酒精性肝损伤模型构建成功。除刺五加低剂量组TG水平与模型组差异无统计学意义(P>0.05)外,与模型组比较,刺五加注射液均能够降低血清中天门冬氨酸氨基转移酶、丙氨酸氨基转移酶和TG水平(P<0.05~P<0.01);抑制小鼠肝脏组织丙二醛含量的升高,提高肝脏中还原型谷胱甘肽含量(P<0.05~P<0.01);减轻肝脏病理组织损伤;刺五加高、中剂量组均能降低小鼠肝脏指数(P<0.05和P<0.01)。结论:刺五加注射液对小鼠酒精性肝损伤具有保护作用,其作用机制可能与抑制氧化应激有关。Abstract: Objective:To explore the protective effects of Acanthopanax senticosus injection on the acute alcoholic hepatic injury in mice.Methods:Sixty Kunming male mice(18 to 22g) were randomly divided into the normal group, model group, Tiopronin positive control group, and Acanthopanax senticosus injection high, medium and low dose group(200 mg/kg, 100 mg/kg and 50 mg/kg)(10 mice each group).The alcoholic hepatic injury model of mice was established by intragastric administration with 12 ml/kg/d of 50% alcohol for 10 days.The levels of serum aspartate transaminase(AST), alanine transferase(ALT), Triglyceride(TG), liver tissue malondialdehyde(MDA) and Glutathione in all groups were detected.Liver index and hepatic pathological changes in all mice were calculated and observed, respectively.Results:The alcoholic hepatic injury model of mice was successfully established.Acanthopanax senticosus injection could reduce the levels of AST, ALT and TG, inhibit the increase of liver MDA, improve the content of reduced glutathione in liver(P<0.05~P<0.01), decrease the liver index and alleviate the liver tissue injury(P<0.05 and P<0.01).Conclusions:Acanthopanax senticosus injection can protect the alcoholic hepatic injury of mice, and the mechanism of which may be related to the suppressing oxidative stress.
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