重型颅脑损伤T淋巴细胞亚群的变化

苗雨露; 张明霞

doi:10.13898/j.cnki.issn.1000-2200.2015.05.021

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重型颅脑损伤T淋巴细胞亚群的变化

苗雨露 , 张明霞

文章导航 > 蚌埠医学院学报 > 2015, 40 > 5: (624-626)

引用本文:

Citation:

重型颅脑损伤T淋巴细胞亚群的变化

苗雨露¹,
张明霞²

1. 广东省深圳市龙岗区第二人民医院神经外科, 518112;
2. 广东省深圳市第三人民医院中心实验室, 518112

作者简介: 苗雨露(1966-),男,硕士,主任医师.

中图分类号: R651.15

Change of T lymphocyte subsets in patients with severe craniocerebral injury

MIAO Yu-lu¹,
ZHANG Ming-xia²

1. Department of Neurosurgery, The Second People's Hospital of Longgang District of Shenzhen, Shenzhen Guangdong 518112;
2. Department of Centre Laboratory, The Third People's Hospital of Shenzhen, Shenzhen Guangdong 518112, China
CLC number: R651.15

摘要: 目的:探讨重型颅脑损伤后T淋巴细胞亚群变化规律,为进行免疫干预预防感染提供参考依据。方法:选择单纯重型颅脑损伤格拉斯哥昏迷评分≤8分的患者30例为观察组,同期单纯轻型颅脑损伤格拉斯哥昏迷评分=14~15分患者30例为对照组。采用免疫荧光三色标记流式细胞仪对2组患者伤后第1、3、7和14天外周血T淋巴细胞亚群进行检测。结果:与对照组比较,观察组伤后第1天CD3、 CD4、CD4/CD8开始下降,CD8升高,第3天CD3、 CD4、CD4/CD8明显下降,CD8明显升高,第7天开始恢复,第14天基本同正常水平,2组伤后第1、3和7天差异均有统计学意义(P<0.05~P<0.01)。而伤后第14天 2组4项指标差异均无统计学意义(P>0.05)。观察组伤后第4天肺部感染73.3%,对照组无肺部感染,2组差异有统计学意义(P<0.01)。结论:重型颅脑损伤后T淋巴细胞亚群变化规律为伤后第1天开始下降,第3天下降明显,第7天开始恢复,第14天基本恢复到正常水平,可考虑重型颅脑损伤后进行免疫干预预防感染。
- 颅脑损伤 /
- T淋巴细胞亚群 /
- 流式细胞仪 /
- 免疫 /
- 感染
Abstract: Objective: To detect the change rules of T lymphocyte subsets in patients with severe craniocerebral injury to provide the basis in preventing infection by immunologic intervention.Methods: Thirty severe craniocerebral injury patients with glasgow coma score(GCS)≤8 and 30 light craniocerebral injury patients GCS =14 to 15 were divided into the observation group and control group,respectively.The expressions of lymphocyte subsets(including CD3,CD4,CD8,CD4/CD8 T) of peripheral blood in two groups after 1,3,7 d and 14 d of injury were detected using the immunofluorescent three-color flow cytometry.Results: Compared with the control group,the levels of CD3,CD4 and CD4/CD8 began to decrease,and the level of CD8 increased 1 d after injury in observation group.Compared with the control group,the levels of CD3,CD4 and CD4/CD8 decreased significantly,and the level of CD8 increased significantly 3 d after injury in observational group.The levels of lymphocyte subsets began to recover after 7 d of injury,and arrived at the normal level after 14 d of injury.The differences of the levels of lymphocyte subsets between two groups after 1 d,3 d and 7 d after injury were statistically significant(P<0.05 to P<0.01),and there was not statistical significance 14 d after injury(P>0.05).The lung infection rates in the observation group and control group after 4 d of injury were 73.3% and 0,respectively,the difference of which was statistically significant(P<0.01).Conclusions: Decreasing on day 1,significant decreasing on day 3,recovering on day 7 and arriving at the normal level on day 14 are the change rules of T lymphocyte subsets in severe craniocerebral injury patients.The immunologic intervention can be used to prevent infection in severe craniocerebral injury patients.
- craniocerebral injury /
- T lymphocyte subsets /
- flow cytometry /
- immune /
- infection

[1]	Mrakovcic-Sutic I,Tokmadzic VS,Laskarin G,et al.Early changes in frequency of peripheral blood lymphocyte subpopulations in severe traumatic brain-injured patients[J].Scand J Immunol,2010,72(1):57-65.
[2]	Sotosek Tokmadzic V,Laskarin G.Expression of cytolytic protein-perforin in peripheral blood lymphocytes in severe traumatic brain injured patients[J].Injury,2012,43(5):624-631.
[3]	张梅,赵云峰,骆兰尼,等.肺炎及英国胸科协会改良肺炎评分及慢性阻塞性费疾病急性加重患者早期病死率的评估价值[J].中华结核和呼吸杂志,2013,36(4):269-273.
[4]	Zhang M,Zhao YF,Luo LN,et al.The value of coexisting pneumonia and British Thoracic Society CURB-65 score in predicting early mortality rate in patients with acute exacerbation of chronic obstructive pulmonary disease[J].Chin J Tuberc Respir Dis,2013,36(4):269-273.
[5]	Zanetti M,Castiglioni P,Ingulli E.Principles of of memory CD8 T-cells generation in relation to protective immunity[J].Adv Exp Med Biol,2010,684:108-125.
[6]	Mu J,Jeyanathan M,Shaler CR,et al.Reapiratory mucosal immunization with adenovirus gene transfer vector induces helper CD4 T cell-independent protective immunity[J].J Gene Med,2010,12(8):693-704.
[7]	Epifantseva NN,Borshchikova TI,Surzhikova GS,et al.The specific features of immune disorders in acute severe brain injury[J].Anesteziol Reanimatol,2009(6):65-68.
[8]	Smrcka M,Mrlian A,Karlsson-Valik J,et al.The effect of head injury upon the immune system[J].Bratisl Lek Listy,2007,108(3):144-148.
[9]	闫仁福,周跃,苏忠周,等.颅脑损伤后T细胞亚群及性激素的动态变化[J].中华创伤杂志,2003,19(12):724-727.
[10]	王文涛,马弟娃,牛小东,等.免疫营养支持对颅脑损伤病人影响的系统评价[J].肠外与肠内营养,2014,21(5):273-281.

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重型颅脑损伤T淋巴细胞亚群的变化

苗雨露¹
张明霞²

作者简介: 苗雨露(1966-),男,硕士,主任医师.

1. 广东省深圳市龙岗区第二人民医院神经外科, 518112;
2. 广东省深圳市第三人民医院中心实验室, 518112

收稿日期: 2014-06-24

关键词:

摘要: 目的:探讨重型颅脑损伤后T淋巴细胞亚群变化规律,为进行免疫干预预防感染提供参考依据。方法:选择单纯重型颅脑损伤格拉斯哥昏迷评分≤8分的患者30例为观察组,同期单纯轻型颅脑损伤格拉斯哥昏迷评分=14~15分患者30例为对照组。采用免疫荧光三色标记流式细胞仪对2组患者伤后第1、3、7和14天外周血T淋巴细胞亚群进行检测。结果:与对照组比较,观察组伤后第1天CD3、 CD4、CD4/CD8开始下降,CD8升高,第3天CD3、 CD4、CD4/CD8明显下降,CD8明显升高,第7天开始恢复,第14天基本同正常水平,2组伤后第1、3和7天差异均有统计学意义(P<0.05~P<0.01)。而伤后第14天 2组4项指标差异均无统计学意义(P>0.05)。观察组伤后第4天肺部感染73.3%,对照组无肺部感染,2组差异有统计学意义(P<0.01)。结论:重型颅脑损伤后T淋巴细胞亚群变化规律为伤后第1天开始下降,第3天下降明显,第7天开始恢复,第14天基本恢复到正常水平,可考虑重型颅脑损伤后进行免疫干预预防感染。

English Abstract

Change of T lymphocyte subsets in patients with severe craniocerebral injury

MIAO Yu-lu¹
ZHANG Ming-xia²

1. Department of Neurosurgery, The Second People's Hospital of Longgang District of Shenzhen, Shenzhen Guangdong 518112;
2. Department of Centre Laboratory, The Third People's Hospital of Shenzhen, Shenzhen Guangdong 518112, China

Received Date: 2014-06-24

Abstract: Objective: To detect the change rules of T lymphocyte subsets in patients with severe craniocerebral injury to provide the basis in preventing infection by immunologic intervention.Methods: Thirty severe craniocerebral injury patients with glasgow coma score(GCS)≤8 and 30 light craniocerebral injury patients GCS =14 to 15 were divided into the observation group and control group,respectively.The expressions of lymphocyte subsets(including CD3,CD4,CD8,CD4/CD8 T) of peripheral blood in two groups after 1,3,7 d and 14 d of injury were detected using the immunofluorescent three-color flow cytometry.Results: Compared with the control group,the levels of CD3,CD4 and CD4/CD8 began to decrease,and the level of CD8 increased 1 d after injury in observation group.Compared with the control group,the levels of CD3,CD4 and CD4/CD8 decreased significantly,and the level of CD8 increased significantly 3 d after injury in observational group.The levels of lymphocyte subsets began to recover after 7 d of injury,and arrived at the normal level after 14 d of injury.The differences of the levels of lymphocyte subsets between two groups after 1 d,3 d and 7 d after injury were statistically significant(P<0.05 to P<0.01),and there was not statistical significance 14 d after injury(P>0.05).The lung infection rates in the observation group and control group after 4 d of injury were 73.3% and 0,respectively,the difference of which was statistically significant(P<0.01).Conclusions: Decreasing on day 1,significant decreasing on day 3,recovering on day 7 and arriving at the normal level on day 14 are the change rules of T lymphocyte subsets in severe craniocerebral injury patients.The immunologic intervention can be used to prevent infection in severe craniocerebral injury patients.