白藜芦醇对食管鳞癌Eca-109细胞侵袭迁移及EMT的影响
Effect of resveratrol on the invasion migration of Eca-109 cells and epithelial-mesenchymal transition in esophageal squamous cell carcinoma
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摘要: 目的:研究白藜芦醇(RES)对食管鳞癌Eca-109细胞侵袭迁移及上皮间质转化(EMT)的影响.方法:用不同浓度(50和100 μmol/L)的RES处理Eca-109细胞,采用细胞划痕实验、Transwell小室实验检测细胞迁移侵袭能力,荧光定量PCR检测EMT蛋白E-cadherin、N-cadherin、Vimentin和MMP-2、MMP-9 mRNA的表达,Western blot检测E-cadherin、N-cadherin、Vimentin、MMP-2和MMP-9蛋白表达.结果:与对照组比较,RES各浓度(50 μmol/L和100 μmol/L)组均可明显抑制Eca-109细胞侵袭迁移(P<0.01),下调N-cadherin、Vimentin、MMP-2、MMP-9 mRNA和蛋白表达(P<0.05~P<0.01),增加E-cadherin mRNA和蛋白表达(P<0.01).结论:RES抑制Eca-109细胞侵袭迁移能力,其机制可能通过下调MMP-2、MMP-9的表达,调控EMT蛋白(E-cadherin、N-cadherin和Vimentin)的表达、抑制上皮间质转化过程.Abstract: Objective:To investigate of the effects of resveratrol(RES) on the invasion migration of Eca-109 cells and epithelial-mesenchymal transition(EMT) in esophageal squamous cell carcinoma.Methods:The Eca-109 cells were treated with different dilutions(50 and 100 μmol/L) of RES,then the wound-healing were performed.The cell migration and invasion was detected by transwell chamber assays.The mRNA and protein expressions of E-cadherin,N-cadherin,Vimentin,MMP-2 and MMP-9 were evaluated using real-time quantitative PCR and Western blot,respectively.Results:Compared with the control group,the other two RES treatment group(50 and 100 μmol/L) could significantly inhibit the Eca-109 cell invasion and migration(P<0.01),increase the mRNA and protein expressions of N-cadherin,Vimentin,MMP-2 and MMP-9(P<0.05 to P<0.01),and increase the mRNA and protein expression of E-cadherin(P<0.01).Conclusions:Resveratrol can inhibit the invasion and migration of Eca-109 cells,which can be caused by down-regulating expressions of the MMP-2 and MMP-9,regulating the expressions of EMT protein(E-cadherin,N-cadherin and Vimentin),and inhibiting the epithelial mesenchymal transition process.
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