姜黄素对肾脏缺血再灌注损伤大鼠模型保护作用的研究
The protective effect of curcumin on the ischemia-reperfusion injury in rats
-
摘要: 目的:探讨姜黄素对大鼠肾脏缺血再灌注损伤的保护作用及机制。方法:将60只SD大鼠随机分为假手术组(A组)、缺血再灌注组(B组)、姜黄素+缺血再灌注组(C组)和姜黄素+缺血再灌注组+锌-原卟啉组(D组),各15只。C组和D组术前5 d腹腔注射姜黄素100 mg/kg,A、B组注射等量的0.9%氯化钠注射液。D组大鼠术前60 min腹腔注射锌-原卟啉20μmol/kg。手术游离并阻断双肾肾蒂血管,60 min后恢复灌注,分别于4 h、12 h、24 h取血检测血清肌酐(SCr)、尿素氮(BUN)和肿瘤坏死因子-α(TNF-α)、血红素氧合酶-1(HO-1)、细胞间黏附分子-1(ICAM-1)的表达,24 h切除肾组织检测肾组织髓过氧化物酶(MPO)活性的表达水平。结果:C组和D组血清SCr、BUN、TNF-α、ICAM-1及肾组织MPO水平均高于A组,但低于B组(P<0.05~P<0.01);HO-1的表达低于A组,高于B组(P<0.05~P<0.01)。D组TNF-α和ICAM-1表达水平在各时间点均高于C组(P<0.05~P<0.01),SCr、BUN及肾组织MPO的表达在12 h和24 h高于C组(P<0.05~P<0.01),在4 h时差异无统计学意义(P>0.05);HO-1在各时间点表达均低于后者(P<0.05~P<0.01)。结论:姜黄素对肾脏缺血再灌注损伤具有保护作用,能够有效减轻肾组织的炎性及氧化应激损害。HO-1可能参与姜黄素对肾脏的保护。Abstract: Objective:To investigate the effects and its mechanism of curcumin Cur on ischemia-reperfusion(I/R) injury in rats.Methods:Sixty rats were randomly divided into the sham operation group(group A), I/R group(group B), curcumin+I/R group(group C) and Cur+I/R+Zn-protoporphyrin group(group D)(15 rats each group).group C and D were intraperitoneally injected with 100 mg/kg of curcumin before 5 days of operation, and group C were intraperitoneally injected with 20μmol/kg of Zn-protoporphyrin before 60 mins of operation.Group A and B were injected with equivalent 0.9% of sodium chloride.Except for group A, the bilateral renal blood vessels in other 3 groups were blocked, and poured into after 60 mins.The levels of serum creatinine(SCr), blood urea nitrogen(BUN), tumor necrosis factor-α(TNF-α), heme oxygenase-1(HO-1), intercellular adhesion molecule-1(ICAM-1) in 4 groups were measured after 4 h, 12 h and 24 h of reperfusion.The kidney tissue was excised, and the levels of myeloperoxidase(MPO) activity in excised kidney tissue was measured.Results:The expression levels of SCr, BUN, TNF-α, ICAM-1 and MPO in group C and group D were higher and lower than those in group A and group B, respectively(P<0.05 to P<0.01).The expression levels of HO-1 in group C and group D were lower and higher than that in group A and group B, respectively(P<0.05 to P<0.01).The expression levels of TNF-αand ICAM-1 at each time-points in group D were higher than that in group C(P<0.05 to P<0.01).The expression levels of SCr and BUN in serum, and MPO in kidney tissue in group D at 12 h and 24 h were higher than that in group C(P<0.05 to P<0.01), the differences of whose at 4 h were not statistically significant(P>0.05), the level of HO-1 at each time-points in group D was lower than that in group C(P<0.05 to P<0.01).Conclusions:Curcumin can protect the kidneys against I/R injury, and effectively relieve the inflammatory reaction and oxidative stress of kidney tissue.HO-1 may be involved in the renal protective mechanism of curcumin.
-
Key words:
- curcumin /
- ischemia-reperfusion /
- heme oxygenase-1 /
- rat
-
[1] JAGETIA GC,AGGARWAL BB. "Spicing up" of the immune system by curcumin[J]. J Clin Immuno,2007,27(1):19. [2] GUZELOGLU M,YALCINKAYA FATMACA S,et al. Beneficial effects of tadalafil on renal I/R injury in rats[J]. Urol Int,2011, 86(2):197. [3] AVASARALA S,ZHANG F,LIU G,et al. Curcumin modulates the inflammatory response and inhibits subsequent fibrosis na mouse model of viral-induced acute respiratory distress syndrome[J]. PloS One,2013,8(2):e57285. [4] BASILE V, BELLUTI S, FERRARI E, et al. bis-Dehydroxy-Curcumin triggers mitochondrial-associated cell death in human colon cancer cells through ER-stress induced autophagy[J]. PloS One,2013,8(1):e53664. [5] THARAKAN B,HUNTER FA,SMYTHE WR,et al. Curcumin inhibits reactive oxygen species formation and vascular hyperpermeability following haemorrhagic shock[J]. Clin Exp Pharmacol Physiol,2010,37(9):939. [6] AZZA S, AMANY A. Curcumin immune-mediated and anti-apoptotic mechanisms protect against renal ischemia/reperfusion and distant organ induced injuries[J]. Int Immunopharmacol, 2011,11(8):992. [7] 李朝芝,吴天鹏,夏瑗瑜,等. 姜黄素预处理对大鼠肾脏缺血再灌注损伤的影响及其机制[J]. 武汉大学学报,2011,32(3):320. [8] LI F,KE NW,CHENG F,et al. The protective mechanism of ligustrazine against renal ischemia/reperfusion injury[J]. J Surg Res,2011,166(2):298. [9] ZHOU T,SUR GZ,ZHANG MJ,et al. Role of adhesion molecules and dendritic cells in rat hepatic/renal ischemia-reperfusion injury and anti-adhesive intervention with anti-P-selectin-EGF domain monoclonal antibody[J]. World J Gastroenterol,2005,11(7):1005. [10] 王焰斌,王小雷,李刚,等. 乌司他丁对深低温停循环主动脉手术患者肾缺血再灌注损伤的影响[J]. 中华麻醉学杂志, 2014,34(3):266. [11] ORUC O, INCI K, AKI FT, et al. Sildenafil attenuates renal ischemia reperfusion injury by decreasing leukocyte infiltration[J]. Acta Histochem,2010,112(4):337. [12] CHOI DE,JEONG JY,LIM BJ,et al. Pretreatment of sildenafil attenuates ischemia-reperfusion renal injury in rats[J]. Am J Physiol Renal Physiol,2009,297(2):362. [13] CHAPMAN AL,MOCATTA TJ,SHIVA S,et al. Ceruloplasmin is an endogenous inhibitor of myeloperoxidase[J]. J Biol Chem, 2013,288(9):6465. [14] 钱小英,金晓盛,邵美琴,等. 乌司他丁联合维拉帕米在大鼠急性肺缺血再灌注损伤中的保护作用[J]. 中华实验外科杂志,2014,31(5):1054. [15] MAHIR K, WOLFGANG K. Myeloperoxidase production by macrophage and risk of atherosclerosis[J]. Curr Atheroscler Rep,2012,14(3):277. [16] 滑峰,王竞,谷俊东,等. 髓过氧化物酶基因G-463A多态性与肺癌易感性关系的Meta[J]. 中国肺癌杂志,2010,13(2):122. [17] 刘海霞,陈淼,杨秀娟,等. 血红素氧合酶-1对过氧化氢损伤的Ⅱ型肺泡上皮细胞凋亡的影响[J]. 中华危重病急救医学, 2014,26(2):110. [18] 庞庆丰,徐文莉,何俊,等. 谷氨酰胺对内毒素血症大鼠肠道损伤的保护作用及对血红素氧合酶-1表达的影响[J]. 中华危重病急救医学,2011,23(2):95. [19] JEONG GS, OH GS, PAE HO, et al. Comparative effects of curcuminoids on endothelial heme oxygenase-1 expression:ortho-methoxy groups are essential to enhance heme oxygenase activity and Protection[J]. Exp Mol Med,2006,38(4):393. [20] 丁红. 姜黄素诱导HO-1表达增强人脐静脉血内皮祖细胞活力及抗氧化损伤能力的实验研究[D]. 泸州:泸州医学院, 2012. -
点击查看大图
计量
- 文章访问数: 3756
- HTML全文浏览量: 385
- PDF下载量: 146
- 被引次数: 0
下载:
