不同液体复苏对失血性休克大鼠对骨髓中髓系抑制细胞数量变化的影响
Effect of different fluid resuscitation of hemorrhagic shock on the number of myeloid-derived suppressor cells in rat bone marrow
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摘要: 目的：探讨不同液体复苏失血性休克大鼠后，不同时间点骨髓中髓系抑制性细胞（MDSCs）的数量变化情况。方法：采用股动脉放血方法建立失血性休克大鼠模型，在液体复苏早期阶段釆用乳酸林格液（乳酸林格液组）、晶胶液（晶胶液组）、7.5%高渗氯化钠（高渗氯化钠组）3种不同液体复苏，其后进行自体血液回输复苏，复苏成功后12、24和48 h脊髓离断法处死大鼠，同时设假手术对照组。处死后，获取大鼠骨髓细胞悬液，采用流式细胞技术观察大鼠骨髓中MDSCs的数量随时间变化的情况。结果：乳酸林格液组大鼠平均动脉压高于晶胶液组（P<0.05）。与假手术对照组比较，各时间点的乳酸林格液组、晶胶液组及高渗氯化钠组大鼠骨髓中MDSCs所占的比例均明显降低（P<0.01）；高渗氯化钠组大鼠各时间点骨髓中MDSCs所占比例较乳酸林格液组和晶胶液组均明显降低（P<0.01）；乳酸林格液组和晶胶液组12 h和24 h骨髓中MDSCs含量差异均无统计学意义（P>0.05），而24 h晶胶液组低于乳酸林格液组（P<0.05）。结论：失血性休克早期液体复苏后，大鼠骨髓中MDSCs的数量随时间的延长先升高后降低，说明液体复苏可能适度抑制MDSCs的数量。与乳酸林格液、晶胶液相比，高渗盐溶液复苏失血性休克时，能够更有效抑制骨髓中MDSCs的数量，对失血性休克后的免疫调节作用较强。Abstract: Objective: To explore the number change of myeloid-derived suppressor cells(MDSCs) in rat bone marrow at different time-points after different fluid resuscitation of hemorrhagic shock.Methods: The rat model of hemorrhagic shock was established through femoral artery bleeding method.In early stage of fluid resuscitation,the rats were recovered with lactated Ringer solution(lactated Ringer solution group),crystal glue(crystal glue group) and 7.5% hypertonic sodium chloride(hypertonic sodium chloride group),then the rats were recovered with autologous blood transfusion.The rats were sacrificed after 12 h,24 h and 48 h of successful resuscitation,and the sham operation rat was set as the control group.The number changes of MDSCs in rat bone marrow were detected using flow cytometry.Results: The MAP in lactate Ringer solution group was higher than that in crystal glue group(P<0.05).Compared with the sham group,the proportion of MDSCs in rat bone marrow in lactate Ringer solution group,crystal glue group and hypertonic sodium chloride group at each time point were significantly lower(P<0.01).The proportion of MDSCs in rat bone marrow in hypertonic sodium chloride group at each time point were significantly lower than that in lactate Ringer solution group and crystal glue group(P<0.01).The differences of the MDSCs between lactate Ringer solution group and crystal glue group at 12 h and 24 h were not statistical significance(P>0.05),and the MDSCs in crystal glue group at 24 h was lower than that in lactate Ringer solution group(P>0.05).Conclusions: After the early fluid resuscitation of hemorrhagic shock,the number of MDSCs in bone marrow of rat increase at first,then decrease with the extend of time,which explains that the fluid resuscitation can moderately inhibit the number of MDSCs.Compared with the Ringer lactate fluid and crystal glue liquid,the hypertonic saline solution can effectively inhibit the number of MDSCs after the hypertonic saline solution resuscitation of hemorrhagic shock,which has strong immunoregulatory effects on hemorrhagic shock.
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