重组人粒细胞集落刺激因子对缺血缺氧性脑损伤新生大鼠凋亡诱导因子表达的影响

路黎娟; 徐梅佳; 董焱

doi:10.13898/j.cnki.issn.1000-2200.2016.12.002

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

重组人粒细胞集落刺激因子对缺血缺氧性脑损伤新生大鼠凋亡诱导因子表达的影响

路黎娟 , 徐梅佳 , 董焱

文章导航 > 蚌埠医学院学报 > 2016, 41 > 12: (1545-1549)

引用本文:

Citation:

重组人粒细胞集落刺激因子对缺血缺氧性脑损伤新生大鼠凋亡诱导因子表达的影响

上海交通大学医学院附属第九人民医院儿科, 上海 201999

作者简介: 路黎娟(1984-),女,主治医师.

基金项目:
上海市高校选拔优秀青年教师专项基金（ZZjdyx12114）
中图分类号: R364.4

Effect of recombinant human granulocyte colony stimulating factor on the expression of apoptosis inducing factor in neonatal rats with hypoxic-ischemic brain damage

Department of Pediatrics, The Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201999, China
CLC number: R364.4

摘要: 目的：研究分析重组人粒细胞集落刺激因子对新生SD大鼠凋亡诱导因子表达的影响。方法：随机选取7日龄SD大鼠36只，随机分为假手术组（A组）、脑缺血再灌注组（B组）和药物治疗组（C组），每组各12只。采用Longa线栓法制作大鼠大脑动脉闭塞再灌注模型，C组大鼠在脑缺血2 h后及24 h时给予药物灌注，其余2组给予大鼠注射等量的0.9%氯化钠注射液。实验结束后，采用Longa 5分制标准评分法在24 h时对大鼠的神经功能进行评分，采用免疫组织化学法对大鼠的组织磷酸化c-jun氨基末端激酶、磷酸化细胞外信号调节蛋白激酶进行检测，同时采用原位末端转移酶标记对神经细胞凋亡、TTC染色测脑梗死体积进行相关检测。对凋亡相关基因的表达及凋亡水平进行评价。结果：A组仅有一小部分凋亡细胞，B、C 2组在灌注24 h后可以发现大量的凋亡细胞，细胞核均呈棕褐色，主要分布在缺血脑组织中周围；C组脑梗死程度较B组减轻（P<0.01）。结论：重组人粒细胞集落刺激因子可以起到保护新生SD大鼠神经细胞的作用，避免发生缺血缺氧性脑损伤，这种糖蛋白与细胞凋亡相关基因的表达有关。
- 脑损伤 /
- 缺血缺氧性 /
- 重组人粒细胞集落刺激因子 /
- 大鼠
Abstract: Objective: To study the effects of recombinant human granulocyte colony stimulating factor(rhG-CSF) on the expression of apoptosis inducing factor(AIF) in neonatal SD rats.Methods: Thirty-six healthy neonatal SD rats with 7 days old were randomly divided into the sham operation group(group A),cerebral ischemia reperfusion group(group B) and drug treatment group(group C)(12 rats each group).The rat cerebral arterial occlusion reperfusion model was made using Longa suture blot method.The group C were injected with drug after two hours and 24 hours of cerebral ischemia,and the other two groups were injected with the same amount of normal saline.At the end of the experiment,the neural function of rat was evaluated by Longa 5 score method,the expressions of extracellular signal regulated protein kinase(p-ERK) and phosphorylated extracellular signal regulated protein kinase(p-JNK) were detected by immunohistochemical method,and the neuronal apoptosis and cerebral infarction volume were detected using Tunel and TTC staining,respectively.The levels of expression and apoptosis of apoptosis related genes were evaluated.Results: A small part of the apoptotic cells in A group and large number of apoptotic cells and brown nuclei in groups B and C after 24 h of reperfusion were found,which distributed mainly around the ischemic brain tissue,and which in C group was less than that in group B(P<0.01).Conclusions: rhG-CSF can protect the neuron of neonatal rat,and avoid the occurrence of ischemic hypoxic brain damage,which is related to the expression of the apoptosis related gene.
- brain injury /
- ischemia and hypoxia /
- recombinant human granulocyte colony stimulating factor /
- rat

[1]	张海娇,阴怀清,栗红.神经节苷脂联合参附注射液对新生大鼠缺氧缺血性脑损伤的保护作用[J].中西医结合心脑血管病杂志,2011,9(5):576.
[2]	张晓燕,杨金升.缺血性脑血管病与丝裂原活化蛋白激酶[J].国外医学:脑血管疾病分册,2015,13(11):854.
[3]	LONGA EZ,WEINSTEIN PR,CARLSON S,et al.Reversible middle cerebral artery occlusion without craniectomy in rats[J].Stroke,1989,20(1):84.
[4]	陈真珍,王凯华,黄龙坚.JAK2/STAT3信号传导通路在脑缺血再灌注损伤中的作用[J].中国实用神经疾病杂志,2013,16(10):29.
[5]	JOHNSTON MV,FATEMI A,WILSON MA,et al.Treatment advances in neonatal neuroprotection and neurointensive care[J].Lancet Neurol,2011,10(4):372.
[6]	付振虹,董蔚,盖鲁粤,等.促红细胞生成素联合粒细胞集落刺激因子治疗大鼠急性心肌梗死[J].南方医科大学学报,2011,31(1):17.
[7]	王昌正,曹诚,马清钧.凋亡诱导因子(AIF)对细胞凋亡的调控[J].生命的化学,2005,25(6):454.
[8]	周卓妍,宋燕燕,林兴会,等.联合应用促红细胞生成素和粒细胞集落刺激因子对早产鼠缺氧缺血性脑损伤的保护作用[J].实用儿科临床杂志,2008,23(6):425.
[9]	徐海发,蒋晶晶,马静萍.重组人粒细胞集落刺激因子对大鼠脑缺血再灌注损伤后p-JAK2及p-STAT3信号通路的影响[J].中国神经免疫学和神经病学杂志,2014,21(4):273.
[10]	IADECOLA C,ALEXANDER M.Cerebral ischemia and inllammation[J].Curr Opin Neurol,2001,14(1):89.

[1]	许克强 . 重组人粒细胞集落刺激因子治疗化疗后白细胞减少的有效性分析. 蚌埠医学院学报, 2014, 38(4): 482-483,486.
[2]	罗鑫刚 , 邓青青 , 肖慧媚 , 裴铮 , 易爱文 , 常燕群 . 低氧后处理体外干预对缺氧缺血性脑损伤新生鼠的作用. 蚌埠医学院学报, 2018, 43(6): 707-709. doi: 10.13898/j.cnki.issn.1000-2200.2018.06.003
[3]	黄丽 , 王春 , 赵士弟 . 针刺预处理对大鼠小脑浦肯野细胞缺血性损伤的影响. 蚌埠医学院学报, 2016, 41(1): 7-9,15. doi: 10.13898/j.cnki.issn.1000-2200.2016.01.002
[4]	赵瑾 , 杨卫东 , 汪思应 . 四逆汤对大鼠心肺复苏后脑组织损伤的影响. 蚌埠医学院学报, 2009, 34(1): 18-20.
[5]	周艺 , 李珍 , 王烈成 , 祝延 , 方芳 , 黄大可 . 白藜芦醇对大鼠局灶性脑缺血再灌注损伤的神经保护作用. 蚌埠医学院学报, 2011, 36(9): 921-924,930.
[6]	徐丙发 , 吴华璞 , 祝晓光 . 姜黄素对大鼠缺血再灌注损伤的保护作用. 蚌埠医学院学报, 2007, 32(6): 637-639,765.
[7]	陈银玲 , 童旭辉 , 唐媛媛 , 齐佳佳 , 杜家如 , 董淑英 . P2X7受体拮抗剂BBG抗大鼠星形胶质细胞缺氧/复氧损伤的作用. 蚌埠医学院学报, 2017, 42(6): 701-705. doi: 10.13898/j.cnki.issn.1000-2200.2017.06.001
[8]	杨卫东 , 陈前芬 . 大鼠心肺复苏后脑损伤模型的建立. 蚌埠医学院学报, 2009, 34(2): 103-105.
[9]	方典 , 梁欢 , 王佳慧 , 汪敬业 , 李正红 , 高琴 . 线粒体细胞色素c释放抑制剂对大鼠全脑缺血再灌注损伤炎症反应的作用. 蚌埠医学院学报, 2022, 47(1): 7-12. doi: 10.13898/j.cnki.issn.1000-2200.2022.01.002
[10]	宋朝彦 , 崔建忠 , 洪军 , 王春生 . 大鼠脑创伤后一氧化氮合酶对学习和记忆功能的影响. 蚌埠医学院学报, 2009, 34(7): 558-562.
[11]	侯晞 , 梁枫 , 李爱剑 , 王灿 , 戴体俊 , 桂常青 . 银杏叶提取物联合依达拉奉对气虚血瘀型脑缺血再灌注损伤大鼠脑组织NO及NOS表达的影响. 蚌埠医学院学报, 2011, 36(7): 673-676.
[12]	王伟 , 陈海锋 . 安宫牛黄丸治疗重型颅脑损伤的神经保护作用及对肠黏膜通透性的影响. 蚌埠医学院学报, 2018, 43(2): 201-205. doi: 10.13898/j.cnki.issn.1000-2200.2018.02.018
[13]	刘继松 , 方勇 , 姚敏 , 俞为荣 , 杜娟 . 重组人粒细胞巨噬细胞集落刺激因子对烫伤大鼠创面愈合及新生血管化的影响. 蚌埠医学院学报, 2012, 36(1): 17-19.
[14]	于影 , 胡杰 , 姜翠荣 , 李正红 , 高琴 . 远距缺血后处理对大鼠急性心肌缺血/再灌注损伤的影响. 蚌埠医学院学报, 2011, 36(2): 109-111,116.
[15]	高连珠 . 预见性护理干预在颅脑损伤手术配合中的应用. 蚌埠医学院学报, 2016, 41(6): 838-839. doi: 10.13898/j.cnki.issn.1000-2200.2016.06.044
[16]	代应辉 , 李寿玲 , 朱美玲 , 陈逖 . 大鼠高眼压视网膜缺血再灌注损伤形态学改变. 蚌埠医学院学报, 2005, 30(5): 385-387.
[17]	姜翠荣 , 高琴 , 王晓梅 , 李正红 . 不同浓度硝酸甘油对大鼠离体心脏缺血/再灌注损伤的作用. 蚌埠医学院学报, 2011, 36(1): 7-10.
[18]	徐卫强 , 郭园园 , 关超 , 方文革 , 查泽玉 . 姜黄素对肾脏缺血再灌注损伤大鼠模型保护作用的研究. 蚌埠医学院学报, 2016, 41(1): 16-19. doi: 10.13898/j.cnki.issn.1000-2200.2016.01.004
[19]	孙硕 , 戎李 , 莫辰 , 张冰 , 高琴 , 王洪巨 , 康品方 , 张恒 . 糖尿病大鼠心肌损伤中程序性坏死通路相关蛋白的表达. 蚌埠医学院学报, 2020, 45(4): 437-441, 446. doi: 10.13898/j.cnki.issn.1000-2200.2020.04.005
[20]	朱海 , 胡建国 , 吕合作 . 流式细胞术检测脊髓损伤大鼠局部浸润的巨噬细胞亚群. 蚌埠医学院学报, 2013, 37(8): 925-928.

点击查看大图

计量

文章访问数: 3671
HTML全文浏览量: 410
PDF下载量: 77
被引次数: 0

重组人粒细胞集落刺激因子对缺血缺氧性脑损伤新生大鼠凋亡诱导因子表达的影响

作者简介: 路黎娟(1984-),女,主治医师.

上海交通大学医学院附属第九人民医院儿科, 上海 201999

收稿日期: 2015-11-30

基金项目: 上海市高校选拔优秀青年教师专项基金（ZZjdyx12114）

关键词:

摘要: 目的：研究分析重组人粒细胞集落刺激因子对新生SD大鼠凋亡诱导因子表达的影响。方法：随机选取7日龄SD大鼠36只，随机分为假手术组（A组）、脑缺血再灌注组（B组）和药物治疗组（C组），每组各12只。采用Longa线栓法制作大鼠大脑动脉闭塞再灌注模型，C组大鼠在脑缺血2 h后及24 h时给予药物灌注，其余2组给予大鼠注射等量的0.9%氯化钠注射液。实验结束后，采用Longa 5分制标准评分法在24 h时对大鼠的神经功能进行评分，采用免疫组织化学法对大鼠的组织磷酸化c-jun氨基末端激酶、磷酸化细胞外信号调节蛋白激酶进行检测，同时采用原位末端转移酶标记对神经细胞凋亡、TTC染色测脑梗死体积进行相关检测。对凋亡相关基因的表达及凋亡水平进行评价。结果：A组仅有一小部分凋亡细胞，B、C 2组在灌注24 h后可以发现大量的凋亡细胞，细胞核均呈棕褐色，主要分布在缺血脑组织中周围；C组脑梗死程度较B组减轻（P<0.01）。结论：重组人粒细胞集落刺激因子可以起到保护新生SD大鼠神经细胞的作用，避免发生缺血缺氧性脑损伤，这种糖蛋白与细胞凋亡相关基因的表达有关。

English Abstract

Effect of recombinant human granulocyte colony stimulating factor on the expression of apoptosis inducing factor in neonatal rats with hypoxic-ischemic brain damage

Department of Pediatrics, The Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201999, China

Received Date: 2015-11-30

Abstract: Objective: To study the effects of recombinant human granulocyte colony stimulating factor(rhG-CSF) on the expression of apoptosis inducing factor(AIF) in neonatal SD rats.Methods: Thirty-six healthy neonatal SD rats with 7 days old were randomly divided into the sham operation group(group A),cerebral ischemia reperfusion group(group B) and drug treatment group(group C)(12 rats each group).The rat cerebral arterial occlusion reperfusion model was made using Longa suture blot method.The group C were injected with drug after two hours and 24 hours of cerebral ischemia,and the other two groups were injected with the same amount of normal saline.At the end of the experiment,the neural function of rat was evaluated by Longa 5 score method,the expressions of extracellular signal regulated protein kinase(p-ERK) and phosphorylated extracellular signal regulated protein kinase(p-JNK) were detected by immunohistochemical method,and the neuronal apoptosis and cerebral infarction volume were detected using Tunel and TTC staining,respectively.The levels of expression and apoptosis of apoptosis related genes were evaluated.Results: A small part of the apoptotic cells in A group and large number of apoptotic cells and brown nuclei in groups B and C after 24 h of reperfusion were found,which distributed mainly around the ischemic brain tissue,and which in C group was less than that in group B(P<0.01).Conclusions: rhG-CSF can protect the neuron of neonatal rat,and avoid the occurrence of ischemic hypoxic brain damage,which is related to the expression of the apoptosis related gene.