不同剂量乙醇对垂体后叶素致家兔急性心肌缺血的影响
Effect of different doses of ethanol on the acute myocardial ischemia induced by pituitrin in rabbits
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摘要: 目的：探讨不同剂量乙醇对急性心肌缺血的影响。方法：36只家兔随机分为6组：对照组、垂体后叶素组、5%乙醇+垂体后叶素组、10%乙醇+垂体后叶素组、20%乙醇+垂体后叶素组、30%乙醇+垂体后叶素组。观察不同给药组血流动力学、心电图指标，实验结束后测定血浆肌酸激酶（CK）、乳酸脱氢酶（LDH）活性。结果：与对照组比较，垂体后叶素组和30%乙醇+垂体后叶素组心电图ST段抬高、T波在1 min、3 min和5 min时幅度均增高，血浆CK、LDH活性均增加（P<0.05）；与垂体后叶素组比较，5%乙醇+垂体后叶素组和10%乙醇+垂体后叶素组ST段、T波幅度均下降，血浆CK和LDH活性均降低（P<0.05）。结论：5%乙醇和10%乙醇对垂体后叶素所致家兔急性心肌缺血有保护作用，而30%乙醇加重垂体后叶素诱导的心肌缺血。Abstract: Objective: To investigate the effects of different doses of ethanol on acute myocardial ischemia in rabbits.Methods: Thirty six rabbits were randomly divided into the control group, pituitrin group,5% ethanol+pituitrin group,10% ethanol+pituitrin group,20% ethanol+pituitrin group and 30% ethanol+pituitrin group.The hemodynamics and electrocardiogram in different groups were observed. After the end of the experiment,the activities of plasma creatine kinase(CK)and lactate dehydrogenase(LDH)were measured.Results: Compared with the cotrol group,the ST segment significantly elevated,the breadth of T wave at 1 min,3 min and 5 min significantly increased,and the activities of CK and LDH significantly increased in pituitrin group and 30% ethanol+ pituitrin group(P<0.05).Compared with pituitrin group,the ST segment and T wave breadth decreased,and the activities of CK and LDH significantly increased in 5% ethanol+ pituitrin group and 10% ethanol+pituitrin group(P<0.05). Conclusions: The 5% and 10% ethanol have protective effects on acute myocardial ischemia induced by pituitrin in rabbits, while 30% ethanol can aggravate the myocardial ischemia.
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Key words:
- myocardial ischemia /
- ethanol /
- pituitrin /
- rabbit
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[1] RAZVODOVSKY YE.Alcohol-attributable fraction of ischemic heart disease mortality in russia[J].Isrn Cardiol,2013,2013:287869. [2] ATHYROS VG,LIBEROPOULOS EN,MIKHAILIDIS DP,et al.Association of drinking pattern and alcohol beverage type with the prevalence of metabolic syndrome,diabetes,coronary heart disease,stroke,and peripheral arterial disease in a Mediterranean cohort [J].Angiology,2008,58(6):689. [3] RONKSLEY PE,BRIEN SE,TURNER BJ,et al.Association of alcohol consumption with selected cardiovascular disease outcomes:a systematic review and meta-analysis[J].BMJ,2011,342(3):671. [4] ZHENG YL,LIAN F,SHI Q,et al.Alcohol intake and associated risk of major cardiovascular outcomes in women compared with men:a systematic review and meta-analysis of prospective observational studies[J].BMC Public Health,2015,15(1):773. [5] 于影,张冠军,宗巧凤,等.低剂量乙醇干预减轻糖尿病大鼠心肌纤维化的机制探讨[J].中国临床药理学与治疗学,2015,20(4):379. [6] 蔡久英,高薇.不同饮酒量对新血管内皮功能及炎性因子的影响[J].中国急救医学,2010,30(9):829. [7] GAZZIERID,TREVISANIM,TARANTINIF,et al.Ethanol dilates coronary arteries and increases coronary flow via transient receptor potential vanilloid 1 and calcitonin gene-related peptide [J].Cardiovasc Res,2006,70(3):589. [8] 卢志强,张艳军,崔广智,等.心肌缺血模型的制作方法研究进展[J].中国药理学通报,2012,28(8):1053. [9] 王磊,彭仁秀,杨静,等.复方缬芎滴丸对犬急性心肌缺血的保护作用[J].中草药,2004,35(3):304. [10] 吴海婴.CK、CKMB、LDH、AST检测在急性心肌梗死诊断中的价值[J].心血管病防治知识(学术版),2015(3):56. [11] SALIM A,TEIXEIRA P,LEY EJ,et al.Serum ethanol levels:predictor of surviva after severe traumatic brain injury [J].Trauma,2009,67(4):697. [12] AMANI M,JEDDI S,AHMADIASL N,et al.Effect of HEMADO on level of CK-MB and LDH enzymes after ischemia/reperfusion injury in isolated rat heart [J].Bioimpacts,2013,3(2):101. [13] 魏林,李培杰.乙醇对家兔心脏骤停后综合征脑保护的实验研究[D].兰州:兰州大学,2013. [14] YU Y,JIA XJ,ZONG QF,et al.Remote ischemic postconditioning protects the heart by upregulating ALDH2 expression levels through the PI3K/Akt signaling pathway[J].Mol Med Rep,2014,4(15):536. [15] 刘玉江.不同浓度乙醇对家兔血压的影响[J].微量元素与健康研究,2011,28(5):10. [16] 卢继承.乙醇对家兔循环系统各项指标的影响[J].泰山学院学报,2016,38(3):103. -
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