不明原因复发性流产病人外周血NK细胞的临床意义

刘佳杰; 吴祖常; 方小武; 韦剑洪; 陈伟辉

doi:10.13898/j.cnki.issn.1000-2200.2017.06.017

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不明原因复发性流产病人外周血NK细胞的临床意义

刘佳杰 , 吴祖常 , 方小武 , 韦剑洪 , 陈伟辉

文章导航 > 蚌埠医学院学报 > 2017, 42 > 6: (752-754)

引用本文:

Citation:

不明原因复发性流产病人外周血NK细胞的临床意义

1. 广东省中山市博爱医院生殖医学中心, 528403;
2 广东省佛山市第一人民医院临床医学研究所, 528000

作者简介: 刘佳杰(1982-),男,主管技师.

中图分类号: R714.2

The clinical significance of peripheral blood natural killer cell in patients with unexplained recurrent spontaneous abortion

1. Reproductive Medicine Center, Zhongshan Boai Hospital, Zhongshan Guangdong 528403;
2. The Clinical Medical Research Institute, The First People's Hospital of Foshan, Foshan Guangdong 528000, China
CLC number: R714.2

摘要: 目的：探讨不明原因复发性流产（URSA）病人难免流产和成功妊娠后外周血NK细胞的变化及临床意义。方法：选择URSA流产病人44例（URSA流产组）、URSA妊娠病人34例（URSA妊娠组）作为观察组，并选择同期正常妊娠者44例（正常妊娠组）、健康非妊娠者44名（正常非妊娠组）作为对照组。使用流式细胞术检测外周血NK细胞的数量。结果：4组研究对象流产1个月后检测NK细胞占淋巴细胞的比例为（21.18±5.81）%、（16.12±4.1）%、（15.16±3.23）%和（28.16±3.38）%；URSA流产组明显高于URSA妊娠组、正常妊娠组及正常非妊娠组（P<0.01），URSA妊娠组、正常妊娠组及正常非妊娠组之间两两比较，差异均无统计学意义（P>0.05）。结论：URSA的发生与NK细胞对胚胎的杀伤增强有关，外周血NK细胞表达水平可作为监测URSA病人的指标。
- 流产 /
- 妊娠 /
- NK细胞
Abstract: Objective: To investigate the changes of peripheral blood natural killer(NK) cell between inevitable abortion and successful pregnancy in patients with unexplained recurrent spontaneous abortion(URSA),and its clinical significance. Methods: Forty-four URSA abortion patients and 34 URSA pregnant patients were divided into the URSA abortion group and URSA pregnancy group,respectively.Forty-four normal pregnancy women and 44 normal non-pregnancy women were divided into the normal pregnancy group and normal non-pregnancy group(control group),respectively.The number of peripheral blood NK cells in four groups was detected using flow cytometry. Results: The ratio of NK cell to lymphocyte in URSA abortion group,URSA pregnancy group,normal pregnancy group and normal non-pregnancy group was(21.18±5.81)%,(16.12±4.13)%,(15.16±3.23)% and(14.91±3.18)%,respectively.The ratio of NK cell to lymphocyte in URSA abortion group was higher than that in URSA pregnancy group,normal pregnancy group and normal non-pregnancy group(P<0.01),the differences of which between URSA pregnancy group,normal pregnancy group and normal non-pregnancy group were not statistically significant(P>0.05). Conclusions: The occurrence of URSA may be associated with the enhancement of NK cells to embryo Rilling.The number of NK cells in peripheral blood can be used as an index in monitoring URSA patients.
- abortion /
- pregnancy /
- natural killer cell

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不明原因复发性流产病人外周血NK细胞的临床意义

作者简介: 刘佳杰(1982-),男,主管技师.

1. 广东省中山市博爱医院生殖医学中心, 528403;
2 广东省佛山市第一人民医院临床医学研究所, 528000

收稿日期: 2016-08-17

关键词:

流产 /
妊娠 /
NK细胞

摘要: 目的：探讨不明原因复发性流产（URSA）病人难免流产和成功妊娠后外周血NK细胞的变化及临床意义。方法：选择URSA流产病人44例（URSA流产组）、URSA妊娠病人34例（URSA妊娠组）作为观察组，并选择同期正常妊娠者44例（正常妊娠组）、健康非妊娠者44名（正常非妊娠组）作为对照组。使用流式细胞术检测外周血NK细胞的数量。结果：4组研究对象流产1个月后检测NK细胞占淋巴细胞的比例为（21.18±5.81）%、（16.12±4.1）%、（15.16±3.23）%和（28.16±3.38）%；URSA流产组明显高于URSA妊娠组、正常妊娠组及正常非妊娠组（P<0.01），URSA妊娠组、正常妊娠组及正常非妊娠组之间两两比较，差异均无统计学意义（P>0.05）。结论：URSA的发生与NK细胞对胚胎的杀伤增强有关，外周血NK细胞表达水平可作为监测URSA病人的指标。

English Abstract

The clinical significance of peripheral blood natural killer cell in patients with unexplained recurrent spontaneous abortion

1. Reproductive Medicine Center, Zhongshan Boai Hospital, Zhongshan Guangdong 528403;
2. The Clinical Medical Research Institute, The First People's Hospital of Foshan, Foshan Guangdong 528000, China

Received Date: 2016-08-17

Abstract: Objective: To investigate the changes of peripheral blood natural killer(NK) cell between inevitable abortion and successful pregnancy in patients with unexplained recurrent spontaneous abortion(URSA),and its clinical significance. Methods: Forty-four URSA abortion patients and 34 URSA pregnant patients were divided into the URSA abortion group and URSA pregnancy group,respectively.Forty-four normal pregnancy women and 44 normal non-pregnancy women were divided into the normal pregnancy group and normal non-pregnancy group(control group),respectively.The number of peripheral blood NK cells in four groups was detected using flow cytometry. Results: The ratio of NK cell to lymphocyte in URSA abortion group,URSA pregnancy group,normal pregnancy group and normal non-pregnancy group was(21.18±5.81)%,(16.12±4.13)%,(15.16±3.23)% and(14.91±3.18)%,respectively.The ratio of NK cell to lymphocyte in URSA abortion group was higher than that in URSA pregnancy group,normal pregnancy group and normal non-pregnancy group(P<0.01),the differences of which between URSA pregnancy group,normal pregnancy group and normal non-pregnancy group were not statistically significant(P>0.05). Conclusions: The occurrence of URSA may be associated with the enhancement of NK cells to embryo Rilling.The number of NK cells in peripheral blood can be used as an index in monitoring URSA patients.