干扰素与核苷（酸）类似物治疗慢性乙型肝炎对病人外周血调节性T细胞影响的差异研究

李伟; 何俊楠; 李冬冬; 赵守松

doi:10.13898/j.cnki.issn.1000-2200.2017.07.011

干扰素与核苷（酸）类似物治疗慢性乙型肝炎对病人外周血调节性T细胞影响的差异研究

蚌埠医学院第一附属医院感染病科, 安徽蚌埠 233004

作者简介: 李伟(1979-),男,硕士,主治医师.

中图分类号: R512.6

Study on the difference of peripheral blood regulatory T lymphocytes in chronic hepatitis B patients treated with antivirus

Department of Infectious Diseases, The First Affiliated Hospital of Bengbu Medical College, Bengbu Anhui 233004, China
CLC number: R512.6

摘要: 目的：探讨干扰素与核苷（酸）类似物治疗慢性乙型肝炎（CHB）对病人外周血调节性T细胞水平影响的差异。方法：10例接受干扰素治疗（干扰素组），13例接受核苷（酸）类似物治疗（核苷组）的CHB病人，治疗前及治疗过程中检测外周血CD4⁺CD25⁺CD127^low/-Tregs比例，并比较其差异性。结果：2组CD4⁺CD25⁺CD127^low/-Treg细胞均呈下降趋势，且差异有统计学意义（P<0.01）。2组治疗开始时、治疗第3个月、第6个月差异均无统计学意义（P>0.05），治疗第9个月时，2组差异有统计学意义（P<0.05）。结论：干扰素对CHB病人外周血CD4⁺CD25⁺CD127^low/-Treg细胞水平变化的影响较核苷（酸）类似物更大，提示其可能具有远期免疫调节作用。
- 乙型肝炎 /
- 调节性T淋巴细胞 /
- 干扰素 /
- 核苷（酸）类似物 /
- 抗病毒治疗
Abstract: Objective:To investigate the difference and significance of the expression of regulatory T cells in peripheral blood of chronic hepatitis B(CHB) patients treated with interferon and nucleotide analogue.Methods:Ten CHB patients were treated with interferon(IFN group),and 13 CHB patients were treated with nucleoside(acid) analogue (nucleoside group).The ratio of the peripheral blood CD4⁺CD25⁺CD127^low/-Tregs was detected before and during treatment,and the difference of which was compared.Results:The CD4⁺CD25⁺CD127^low/-Treg cells in two groups showed a downward trend,the difference between two groups was statistically significant(P<0.01).The differences of cells at the beginning of treatment,and at 3 and 6 months of treatment between two groups were not statistically significant(P>0.05),and the difference of which at 9 months of treatment between two groups was statistically significant(P<0.05).Conclusions:The effect of interferon on the level of CD4⁺CD25⁺CD127^low/-Treg cells in peripheral blood of patients with CHB is greater than that of nucleoside analogue,which suggests that it may have long-term immunomodulatory effects.
- hepatitis B /
- regulatory T lymphocyte /
- interferon /
- nucleoside(acid) analogue /
- antiviral therapy

[1]	OTT JJ,STEVENS GA,GROEGER J,et al.Global epidemiology of hepatitis B virus infection:new estimates of age-specific HBsAg seroprevalence and endemicity[J].Vaccine,2012,30(12):2212.
[2]	European Association for the Study of the Liver.EASL clinical practice guidelines:management of chronic hepatitis B virus infection[J].J Hepatol,2012,57(1):167.
[3]	LIAW YF,KAO JH,PIRATVISUTH T,et al.Asian-Pacific consensus statement on the management of chronic hepatitis B:a 2012 update[J].Hepatol Intern,2012,6(3):531.
[4]	DURANTEL D,ZOULIM F.Innate response to hepatitis B virus infection:observations challenging the concept of a stealth virus[J].Hepatology,2009,50(6):1692.
[5]	RALAINIRINA N,POLI A,MICHEL T,et al.Control of NK cell functions by CD4⁺CD25⁺regulatory T cells[J].J Leukoc Biol,2007,81(1):144.
[6]	GUO PP,LI SP,WU W,et al.Circulating CD4⁺CD25⁺regulatory T cells correlate with chronic hepatitis B infection[J].Immunology,2008,123(1):57.
[7]	PARK HJ,PARK JS,JEONG YH,et al.PD-1 Upregulated on regulatory T cells during chronic virus infection enhances the suppression of CD8⁺T cell immune response via the interaction with PD-L1 expressed on CD8⁺T cells[J].J Immunol,2015,194(12):5801.
[8]	JONES A,OPEJIN A,HENDERSON JG,et al.Peripherally induced tolerance depends on peripheral regulatory T cells that require hopx to inhibit intrinsic IL-2 expression[J].J Immunol,2015,195(4):1489.
[9]	SAKAGUCHI S.Naturally arising Foxp3-expressing CD25⁺CD4⁺regulatory T cells in immunological tolerance to self and non-self[J].Nat Immunol,2005,6(4):345.
[10]	LIU W,PUTNAM AL,XU-YU Z,et al.CD127 expression inversely correlates with FoxP3 and suppressive function of human CD4⁺ T reg cells[J].J Exp Med,2006,203(7):1701.
[11]	MA H,ZHANG HH,WEI L.Frequency of T-cell FoxP3(+) Treg and CD4(+)/CD8(+) PD-1 expression is related to HBeAg seroconversion in hepatitis B patients on pegylated interferon[J].Chin Med J (Engl),2013,126(2):267.
[12]	YU XP,GUO RY,SU ML,et al.Dynamic changes of Treg and Th17 cells and related cytokines closely correlate with the virological and biochemical response in chronic hepatitis B patients undergoing nucleos(t)ide analogues treatment[J].Hepat Mon,2013,13(12):e15332.
[13]	潘修成,杨帆,陈明,等.替比夫定对慢性乙型肝炎患者外周血CD4⁺CD25⁺调节性T细胞动态变化的影响及其临床意义[J].中华肝脏病杂志,2008,16(12):885.
[14]	STOOP JN,VANDER MOLEN RG,KUIPERS EJ,et al.Inhibition of viral replication reduces regulatory T cells and enhances the antiviral immune response in chronic hepatitis B[J].Virology,2007,361(1):141.
[15]	YAN Z,ZHOU J,ZHANG M,et al.Telbivudine decreases proportion of peripheral blood CD4⁺CD25⁺CD127^low T cells in parallel with inhibiting hepatitis B virus DNA[J].Mol Med Rep,2014,9(5):2024.

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干扰素与核苷（酸）类似物治疗慢性乙型肝炎对病人外周血调节性T细胞影响的差异研究

作者简介: 李伟(1979-),男,硕士,主治医师.

Study on the difference of peripheral blood regulatory T lymphocytes in chronic hepatitis B patients treated with antivirus

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干扰素与核苷（酸）类似物治疗慢性乙型肝炎对病人外周血调节性T细胞影响的差异研究

作者简介: 李伟(1979-),男,硕士,主治医师.

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Study on the difference of peripheral blood regulatory T lymphocytes in chronic hepatitis B patients treated with antivirus

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干扰素与核苷（酸）类似物治疗慢性乙型肝炎对病人外周血调节性T细胞影响的差异研究

作者简介: 李伟(1979-),男,硕士,主治医师.

Study on the difference of peripheral blood regulatory T lymphocytes in chronic hepatitis B patients treated with antivirus

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干扰素与核苷（酸）类似物治疗慢性乙型肝炎对病人外周血调节性T细胞影响的差异研究

作者简介: 李伟(1979-),男,硕士,主治医师.

English Abstract

Study on the difference of peripheral blood regulatory T lymphocytes in chronic hepatitis B patients treated with antivirus

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