色素内镜联合肿瘤标志物检测在早期结直肠癌诊断中的应用
The value of stain endoscope combined with tumor biomarker in early diagnosis of colorectal cancer
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摘要: 目的：探讨色素内镜联合肿瘤标志物检测对早期结直肠癌诊断的意义。方法：选择Duck's分期A、B期结直肠肿瘤病人30例为观察组；30例结直肠息肉经镜下切除病人作为对照组。用逆转录-聚合酶链式反应和Western blotting检测肿瘤组织癌胚抗原、Survivin在基因水平和蛋白水平的表达。结果：观察组30例病人中，黏膜内腺癌者8例，腺癌者22例，术后Duck's分期A期12例，Duck's分期B期18例。癌胚抗原在Duck's分期A期和B期病人中的表达率分别为25.00%和27.78%，Survivin在Duck's分期A期和B期病人中的表达率分别为41.67%和55.56%。对照组30例良性肿瘤性息肉者，管状腺瘤22例，绒毛状腺瘤6例，管状绒毛状腺瘤2例，癌胚抗原、Survivin均未表达，表达率均低于观察组（P<0.05~P<0.01）。此外，观察组内Survivin表达率显著高于癌胚抗原（P<0.01）。结论：Survivin在早期结直肠癌组织中高表达，可作为联合色素内镜诊断结直肠癌的标志物，对结直肠癌早期诊断与治疗具有重大意义。Abstract: Objective:To investigate the clinical effects of stain endoscope combined with tumor biomarker in early diagnosis of colorectal cancer.Methods:According to Duck's staging,30 patients with stage Duck's A and B colorectal cancer were set as the observation group,and 30 colorectal polyp patients were set as the control group.The gene and protein expressions of carcinoembryonic antigen and Survivin in two groups were detected using RT-PCR and Western blotting,respectively.Results:Among 30 colorectal cancer patients,intramucosal adenocarcinom in 8 cases and adenocarcinoma in 22 cases were found,and stage Duck's A in 12 cases and stage Duck's B in 18 cases were identified after operation.The expression rates of carcinoembryonic antigen and Survivin at stage Duck's A were 25.00% and 27.78%,and the expression rates of carcinoembryonic antigen and Survivin at stage Duck's B were 41.67% and 55.56%.Among 30 polyp patients,canalicular adenoma in 22 cases,villous adenoma in 6 cases and tubulovillous adenoma in 2 cases were found.The expressions of carcinoembryonic antigen and Survivin in control group were not found,which was lower than that in observed group(P<0.05 to P<0.01).The expression rate of Survivin in observed group was significantly higher than that of carcinoembryonic antigen(P<0.01).Conclusions:The Survivin expression is high in early stage of colorectal cancer,which can set as a potential biomarker in combing with stain endoscope,and has important significance in the early diagnosis and treatment of colorectal cancer.
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Key words:
- colorectal neoplasms /
- stain endoscope /
- carcinoembryonic antigen /
- Survivin
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[1] WENHAO W,JUNLAN F,HUANLONG Q,et al.Molecular therapy of colorectal cancer:progress and future directions[J].Int Cancer,2015,136(3):493. [2] THAKKAR K,FISHMAN DS,GILGER MA.Colorectal polyps in childhood[J].Curr Opin Pediatr,2012,24(5):632. [3] DESAI D,NUTAN D.Colorectal cancer surveillance in inflammatory bowel disease:a critical analysis[J].World J Gastrointest Endosc,2014,6(11):541. [4] FEDYANIN M,TRYAKIN A,BYBARAVA A,et al.Maintenance therapy following first-line chemotherapy in metastatic colorectal cancer:toxicity and efficacy-single-institution experience[J].Med Oncol,2015,32(1):429. [5] SHINTANI M,SANGAWA A,YAMAO N,et al.Smac/DIABLO expression in human gastrointestinal carcinoma:association with clinicopathological parameters and survivin expression[J].Oncol Lett,2014,8(6):2581. [6] 张亚历,周殿元.重视肠镜检查方法,提高早期大肠癌的诊断水平[J].胃肠病学,2002,7(4):253. [7] 周雪涛.内镜下早期大肠癌的诊断与治疗[J].中外医学研究,2011,9(26):161. [8] QINGQUAN S,QIZHEN W,JIANKANG Z,et al.Loss of membranous carcinoembryonic antigen-related cell adhesion molecule 1 expression is related to decreased relapse-free survival of hepatocellular carcinoma following liver transplantation[J].Chin Med J,2012,125(16):2841. [9] 姜泊.染色内镜和放大内镜技术是提高早期大肠癌诊治水平的重要手段[J].第一军医大学学报,2002,22(5):385. [10] 李国新,赵丽瑛.腹腔镜结直肠癌根治术解剖概要[J].中国实用外科杂志,2011(9):844. [11] 朱建新.应用染色内镜和放大内镜提高内镜诊断水平[J].中华消化内镜杂志,2003,20(1):7. [12] 季圣昉,钟捷,马天乐,等.色素放大结肠镜诊断结直肠隆起性病变的临床应用[J].中华消化杂志,2002,22(7):31. [13] 黄响玲,钟祥柱,王曦钟,等.色素内镜在大肠早期肿瘤性病变诊断中的应用[J].广东医学院学报,2007,25(1):41. [14] 甘晓协,张达容,刘预.CEA、CA19-9、TSGF在部分肿瘤诊断中的意义[J].国际检验医学杂志,2005,26(6):380. [15] 张会涛,刘晓珺,沈滨,等.结直肠癌外周血肿瘤标志物的研究进展[J].世界华人消化杂志,2011(10):991. [16] 赵己未,曾晖,邹冰,等.肿瘤标志物CEA、CA19-9在人结直肠癌检测中价值[J].现代消化及介入诊疗,2011,16(2):109. [17] 杨冬.SURVIVIN与胃肠道恶性肿瘤[J].国际消化病杂志,2003,23(3):155. [18] ROHAYEM J,DIESTELKOETTER P,WEIGLE B,et al.Antibody response to the tumor-associated inhibitor of apoptosis protein survivin in cancer patients[J].Cancer Res,2000,60(7):1815. -
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