EV71感染手足口病患儿血清白细胞介素-37和白细胞介素-33水平检测

周庆; 刘倩

doi:10.13898/j.cnki.issn.1000-2200.2018.01.023

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EV71感染手足口病患儿血清白细胞介素-37和白细胞介素-33水平检测

周庆 , 刘倩

文章导航 > 蚌埠医学院学报 > 2018, 43 > 1: (78-80)

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EV71感染手足口病患儿血清白细胞介素-37和白细胞介素-33水平检测

周庆,
刘倩^,

南京中医药大学附属徐州中心医院检验科, 江苏徐州 221009

作者简介: 周庆(1984-),男,检验技师.

通讯作者: 刘倩, 主管技师:3912652@163.com

中图分类号: R512.5

The serum levels of interleukin-37 and interleukin-33 in children with hand-foot-mouth disease caused by enterovirus 71 infection

ZHOU Qing,
LIU Qian^,

Department of Clinical Laboratory, The Affiliated Xuzhou Central Hospital of Nanjing University of Traditional Chinese Medicine, Xuzhou Jiangsu 221009, China

Corresponding author: LIU Qian, 主管技师:3912652@163.com

CLC number: R512.5

摘要: 目的：研究EV71感染手足口病（HFMD）患儿血清白细胞介素（IL）-37和IL-33水平并分析其意义。方法：收集180例确诊的EV71感染HFMD患儿（观察组）和100名健康体检儿童（对照组）外周血，采用全自动血球分析仪检测外周血中性粒细胞和单核细胞绝对值，采用ELISA法检测血清IL-37和IL-33水平，并分析其相关性。结果：观察组外周血中性粒细胞绝对值、单核细胞绝对值、血清IL-37水平和血清IL-33水平均显著高于对照组（P<0.01）；重症组EV71感染HFMD患儿外周血中性粒细胞绝对值、单核细胞绝对值、血清IL-37水显著平均高于轻症组（P<0.05~P<0.01）；观察组血清IL-37与IL-33水平呈正相关关系（P<0.05）；重症组血清IL-37水平与外周血单核细胞绝对值呈正相关关系（P<0.05）；重症组血清IL-33水平与外周血中性粒细胞绝对值呈正相关关系（P<0.05）。结论：EV71感染手足口病重症患儿IL-37、IL-33水平改变可能与手足口病发病及病情发展有关。
- 手足口病 /
- EV71 /
- 白细胞介素-37 /
- 白细胞介素-33
Abstract: Objective:To detect the serum levels of interleukin(IL)-37 and IL-33 in children with hand-foot-mouth disease(HFMD) caused by enterovirus 71 infection,and analyze its clinical significance.Methods:The absolute values of peripheral blood neutrophils and monocytes,and serum levels of IL-37 and IL-37 in 180 children with HFMD infected by EV71(observation group) and 100 healthy children(control group) were detected using the automatic blood cell analyzer and ELISA,respectively,and the correlations were analyzed.Results:The absolute values of neutrophils and monocytes,and serum levels of IL-37 and IL-33 in observation group were significantly higher than those in control group(P<0.01).The absolute values of neutrophils and monocytes,and serum level of IL-37 in children with severe EV71 infection were higher than those in mild EV71 infection children(P<0.05 to P<0.01).The serum levels of IL-37 in observation group were positive correlation with the serum level of IL-33(P<0.05).In severe group,the serum level of IL-37 was positively correlated to the absolute value of monocytes(P<0.05),and the serum level of IL-33 was positively correlated to the absolute value of neutrophils(P<0.05).Conclusions:The changes of IL-37 and IL-33 levels in children with HFMD infected by EV71 may be related to the pathogenesis of HFMD.
- hand-foot-mouth disease /
- enterovirus 71 /
- interleukin-37 /
- interleukin-33

[1]	MA E,FUNG C,YIP SH,et al.Estimation of the basic reproduction number of enterovirus 71 and coxsackievirus A16 in hand,foot,and mouth disease outbreaks[J].Pediatr Infect Dis J,2011,30(8):675.
[2]	MCMINN PC.An overview of the evolution of enterovirus 71 and its clinical and public health significance[J].FEMS Microbiol Rev,2002,26(1):91.
[3]	WANG SM,LIU CC,TSENG HW,et al.Clinical spectrum of enterovirus 71 infection in children in southern Taiwan,with an emphasis on neurological complications[J].Clin Infect Dis,1999,29(1):184.
[4]	KOK CC.Therapeutic and prevention strategies against human enterovirus 71 infection[J].World J Virol,2015,4(2):78.
[5]	FUJIMOTO T,CHIKAHIRA M,YOSHIDA S,et al.Outbreak of central nervous system disease associated with hand,foot,and mouth disease in Japan during the summer of 2000:detection and molecular epidemiology of enterovirus 71[J].Microbiol Immunol,2002,46(9):621.
[6]	CHEN ZF,LI RQ,XIE ZC,et al.IL-6,IL-10 and IL-13 are associated with pathogenesis in children with Enterovirus 71 infection[J].Int J Clin Exp Med,2014,7(9):2718.
[7]	YE N,GONG X,PANG LL,et al.Cytokine responses and correlations thereof with clinical profiles in children with enterovirus 71 infections[J].BMC Infect Dis,2015,15:225.
[8]	HAN J,WANG Y,GAN X,et al.Serum cytokine profiles of children with human enterovirus 71-associated hand,foot,and mouth disease[J].J Med Virol,2014,86(8):1377.
[9]	ZHANG Y,LIU H,WANG L,et al.Comparative study of the cytokine/chemokine response in children with differing disease severity in enterovirus 71-induced hand,foot,and mouth disease[J].PLoS One,2013,28,8(6):e67430.
[10]	WANG C,ZHOU R,ZHANG Z,et al.Intrinsic apoptosis and proinflammatory cytokines regulated in human astrocytes infected with enterovirus 71[J].J Gen Virol,2015,96(10):3010.
[11]	STRIZ I.Cytokines of the IL-1 family:recognized targets in chronic inflammation underrated in organ transplantations[J].Clin Sci (Lond),2017,131(17):2241.
[12]	NOLD MF,NOLD-PETRY CA,ZEPP JA,et al.IL-37 is a fundamental inhibitor of innate immunity[J].Nat Immunol,2010,11(11):1014.
[13]	SALMOND RJ,MIRCHANDANI AS,BESNARD AG,et al.IL-33 induces innate lymphoid cell-mediated airway inflammation by activating mammalian target of rapamycin[J].J Allergy Clin Immunol,2012,130(5):1159.

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[16]	陈亚明 , 李猛 , 刘连城 , 吴追文 , 马进 . 手足口病肺部并发症的X线影像表现分析. 蚌埠医学院学报, 2010, 35(6): 619-621.
[17]	李莹 , 李社会 . 硝普钠治疗重症手足口病合并高血压患儿疗效观察. 蚌埠医学院学报, 2015, 40(5): 599-601. doi: 10.13898/j.cnki.issn.1000-2200.2015.05.012
[18]	梁正娟 , 马鑫 , 王绪韶 , 杨惠泉 . 手足口病合并左上肢瘫痪1例. 蚌埠医学院学报, 2010, 35(9): 947-947.
[19]	赵梅 , 刘心洁 . 手足口病并发脑炎患儿水通道蛋白4水平变化及其与炎症因子、神经损伤指标相关性. 蚌埠医学院学报, 2020, 45(6): 757-759, 763. doi: 10.13898/j.cnki.issn.1000-2200.2020.06.015
[20]	李传杰 , 张雅明 , 王亮亮 . 小剂量甲泼尼龙琥珀酸钠早期治疗手足口病疗效观察. 蚌埠医学院学报, 2010, 35(12): 1270-1271.

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EV71感染手足口病患儿血清白细胞介素-37和白细胞介素-33水平检测

周庆
刘倩^,

通讯作者: 刘倩, 主管技师:3912652@163.com

作者简介: 周庆(1984-),男,检验技师.

南京中医药大学附属徐州中心医院检验科, 江苏徐州 221009

收稿日期: 2017-10-16
录用日期: 2017-12-25

关键词:

摘要: 目的：研究EV71感染手足口病（HFMD）患儿血清白细胞介素（IL）-37和IL-33水平并分析其意义。方法：收集180例确诊的EV71感染HFMD患儿（观察组）和100名健康体检儿童（对照组）外周血，采用全自动血球分析仪检测外周血中性粒细胞和单核细胞绝对值，采用ELISA法检测血清IL-37和IL-33水平，并分析其相关性。结果：观察组外周血中性粒细胞绝对值、单核细胞绝对值、血清IL-37水平和血清IL-33水平均显著高于对照组（P<0.01）；重症组EV71感染HFMD患儿外周血中性粒细胞绝对值、单核细胞绝对值、血清IL-37水显著平均高于轻症组（P<0.05~P<0.01）；观察组血清IL-37与IL-33水平呈正相关关系（P<0.05）；重症组血清IL-37水平与外周血单核细胞绝对值呈正相关关系（P<0.05）；重症组血清IL-33水平与外周血中性粒细胞绝对值呈正相关关系（P<0.05）。结论：EV71感染手足口病重症患儿IL-37、IL-33水平改变可能与手足口病发病及病情发展有关。