多聚赖氨酸表面改性后同种异体骨的骨传导能力研究
Study on the osteoconduction of the allograft bone surface-modified by polylysine
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摘要: 目的：探讨多聚赖氨酸表面改性兔同种异体骨后骨传导能力的变化。方法：采用冻干法，将多聚赖氨酸覆盖在实验组兔同种异体骨的微孔表面，对其进行表面改性，并将未经表面改性的同种异体骨设为对照组。兔骨髓基质干细胞（BMSCs）种植于2组的微孔表面，进行培养，并向成骨方向诱导。采用扫描电镜观察、MTT法检测BMSCs在同种异体骨微孔表面黏附、增殖情况，检测2组细胞表达的碱性磷酸酶的活性；将2组复合自体BMSCs的同种异体骨回植入兔双侧桡骨缺损处，1、2、3和4个月后分别对标本进行螺旋CT三维重建，观察新骨生成情况。结果：实验组中BMSCs在同种异体骨微孔表面黏附、增殖以及表达碱性磷酸酶的情况明显优于对照组（P<0.01），实验组兔桡骨缺损处的修复情况优于对照组（P<0.01）。结论：同种异体骨经多聚赖氨酸表面改性后，其在体内和体外的骨传导能力得到了显著的增强。Abstract: Objective:To investigate the osteoconduction of the rabbit's allograft bone surface-modified by polylysine.Methods:The surface of the micropores of the rabbit allograft bone was modified with polylysine using the lyophilization method(experimenal group),and the un-modified surface of the micropores of the rabbit allograft bone was set as control group.The rabbits' BMSCs were implanted into the surface of the micropores of the allograft bone in two groups,cultured and induced to osteoblasts.The attachment and proliferation of the cells on the surface of the micropores in the allograft bone in two groups were detected using SEM and MTT method,respectively.The activity of the alkaline phosphatase in two groups were evaluated.The allograft bone complicated with autologous cell was implanted into the bilateral radiuses defect of rabbits.The specimens were reconstructed by spiral CT after 1,2,3 and 4 months of treatment,and the regenerated bone was observed.Results:The attachment,proliferation and expression of ALP in experimental group were obviously better than those in control group(P<0.01).The recovery of defect lesion in experimental group was better than that in control group(P<0.01).Conclusions:The osteoconduction of the allograft bone surface-modified by polylysine can be significantly strengthened in vivo and in vitro.
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Key words:
- allograft bone /
- osteoconduction /
- polylysine /
- surface modification
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