中国南海硇洲岛海洋微生物抗血栓菌株的筛选及其对血液凝固特性的影响
Screening of marine microbial antithrombotic strains from Naozhou island in South China Sea,and its effect on blood coagulation characteristics
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摘要: 目的：筛选中国南海硇洲岛海洋微生物抗血栓菌株并研究其对血液凝固特性的影响。方法：通过体外溶栓实验筛选抗血栓菌株，然后研究筛选菌株对胶原蛋白-肾上腺素诱导的小鼠尾静脉血栓的形成、小鼠出血时间与凝血时间及对凝血酶诱导的猪全血血小板聚集的影响。结果：体外溶栓实验筛选出了两株具有明显溶栓作用的菌株为腐生葡萄球菌（Mb19）与乙酰微小杆菌（Lmb14）。Mb19与Lmb14高剂量（1 mL）上清液能较好抑制胶原蛋白-肾上腺素诱导的小鼠血栓的形成，与0.9%氯化钠注射液比较差异有统计学意义（P < 0.05）。Mb19与Lmb14高剂量组（1 mL）上清液能有效延长小鼠的出血与凝血时间，与0.9%氯化钠注射液比较差异有统计学意义（P < 0.01）。Mb19与Lmb14高剂量组（100 μL）上清液均能抑制血小板的聚集。结论：海洋细菌腐生葡萄球菌（Mb19）与乙酰微小杆菌（Lmb14）具有抗血栓的作用。Abstract: Objective: To screen the marine microbial antithrombotic strains from Naozhou island in South China Sea,and study its effects on blood coagulation characteristics.Methods: The antithrombotic strains were screened by in vitro thrombolysis,and the effects of the screened strains on collagen-adrenergic-induced tail vein thrombosis,bleeding time and clotting time of mice,and thrombin-induced platelet aggregation of pig were investigated.Results: The in vitro thrombolysis experiments showed that the Staphylococcus saprophyticus(Mb19) and Acetobacter pylori(Lmb14) had obvious thrombolytic effect.The high-dose of supernatants(1 mL) of Mb19 and Lmb14 could effectively inhibit the formation of collagen-adrenergic-induced thrombosis in mouse,and the difference between which and 0.9% sodium chloride injection was statistically significant(P<0.05).The high-dose of supernatants(1 mL) of Mb19 and Lmb14 could effectively prolong the bleeding and clotting time of mice,and the difference between which and 0.9% sodium chloride injection was statistically significant(P<0.01).The high-dose of supernatants(1 mL) could inhibit the platelet aggregation.Conclusions: Mb19 and Lmb14 marine bacteria have the activity of anti-thrombosis.
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Key words:
- marine microorganism /
- thrombus /
- Staphylococcus saprophyticus /
- Acetobacter pylori
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