系统性红斑狼疮病人外周血浆细胞样树突状细胞中Toll样受体7的表达及其意义

刘梦洁; 王涛; 李柏青; 谢长好; 唐洁; 汪洪涛; 李志军

doi:10.13898/j.cnki.issn.1000-2200.2018.10.025

系统性红斑狼疮病人外周血浆细胞样树突状细胞中Toll样受体7的表达及其意义

1. 蚌埠医学院第一附属医院风湿免疫科, 安徽蚌埠 233004;
2. 蚌埠医学院安徽省感染免疫重点实验室, 安徽蚌埠 233030

作者简介: 刘梦洁(1992-),女,硕士研究生.

基金项目:
蚌埠医学院自然科学研究重点项目（BYKY1427ZD）；国家高技术研究发展项目计划（863计划）（2012AA02A513）；蚌埠医学院研究生科研创新项目（Byycx1725）
中图分类号: R593.241

The expression of Toll-like receptor 7 in peripheral plasmacytoid dendritic cells in patients with systemic lupus erythematosus,and its significance

1. Department of Rheumatology, The First Affiliated Hospital of Bengbu Medical College, Bengbu Anhui 233004;
2. Anhui Key Laboratory of Infection Immunity, Bengbu Medical College, Bengbu Anhui 233030, China
CLC number: R593.241

摘要: 目的：检测系统性红斑狼疮（SLE）病人外周血中浆细胞样树突状细胞（pDC）中Toll样受体7（TLR7）的表达，探讨其意义。方法：选取SLE病人52例（SLE组）和正常健康人28名（对照组），利用流式细胞术检测外周血TLR7+的pDC细胞在各组中的比例。结果：SLE病人外周血单核细胞（PBMC）中pDC相对计数显著低于对照组（P<0.01），SLE组和正常对照组髓样树突状细胞水平差异无统计学意义（P>0.05）。感染组SLE病人外周血pDC水平显著低于非感染组（P<0.01），初发组SLE病人外周血髓样树突状细胞水平低于复发组（P<0.05），pDC水平与外周血CRP呈负相关关系（P<0.05）。SLE病人外周血PBMC内pDC中TLR7+细胞的比例显著高于对照组（P<0.01）。血液系统受累组pDC内TLR7+细胞比例高于无血液系统受累组（P<0.05）。TLR7+pDC细胞比例在抗dsDNA抗体、抗Sm抗体、抗SSA抗体、抗SSB抗体阳性组及阴性组之间差异均无统计学意义（P>0.05）；TLR7+pDC细胞比例和蛋白尿分级呈显著正相关关系（P<0.01）。结论：SLE病人外周血TLR7+的pDC细胞比例显著升高，且与临床病情活动性有一定的相关性。
- 系统性红斑狼疮 /
- 浆细胞样树突状细胞 /
- Toll样受体7
Abstract: Objective:To investigate the expression of Toll like receptor 7(TLR7) in peripheral plasmacytoid dendritic cells(pDC) of patients with systemic lupus erythematosus(SLE),and its significance.Methods:Fifty two patients with SLE and 28 healthy persons were divided into the SLE group and control group,respectively.The proportion of TLR7+ pDC in peripheral blood were measured using flow cytometry.Results:The relative count of pDC in peripheral blood monouclear cells(PBMC) of SLE patients was significantly lower than that in control group(P<0.01),and there was no statistical significance in the relative count of myeloid dendritic cells(mDC) between SLE group and control group(P>0.05).The level of peripheral blood pDC in SLE patients was significantly lower than that in non-infected patients(P<0.01),and the level of peripheral blood mDC in patients with primary SLE was lower than that in recurrent patients(P<0.05),and there was a negative correlation between pDC level and CRP(P<0.05).The proportion of TLR7+ pDC in peripheral blood in SLE patients was significantly higher than that in control group(P<0.01).The proportion of TLR7+ pDC of SLE patients with hematological involvement was higher than that in patients without hematological involvement(P<0.05).There was no statistical significance in the proportion of TLR7+ pDC between anti dsDNA antibody,anti Sm antibody,anti SSA antibody,anti SSB antibody positive group and negative group(P>0.05).There was a positive correlation between the proportion of TLR7+ pDC and grade of proteinuria(P<0.01).Conclusions:The proportion of TLR7+ pDC in peripheral blood of patients with SLE significantly increases,and which is correlated with the disease activity.
- systemic lupus erythematosus /
- plasmacytoid dendritic cell /
- Toll like receptor 7

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系统性红斑狼疮病人外周血浆细胞样树突状细胞中Toll样受体7的表达及其意义

作者简介: 刘梦洁(1992-),女,硕士研究生.

The expression of Toll-like receptor 7 in peripheral plasmacytoid dendritic cells in patients with systemic lupus erythematosus,and its significance

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系统性红斑狼疮病人外周血浆细胞样树突状细胞中Toll样受体7的表达及其意义

作者简介: 刘梦洁(1992-),女,硕士研究生.

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The expression of Toll-like receptor 7 in peripheral plasmacytoid dendritic cells in patients with systemic lupus erythematosus,and its significance

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系统性红斑狼疮病人外周血浆细胞样树突状细胞中Toll样受体7的表达及其意义

作者简介: 刘梦洁(1992-),女,硕士研究生.

The expression of Toll-like receptor 7 in peripheral plasmacytoid dendritic cells in patients with systemic lupus erythematosus,and its significance

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系统性红斑狼疮病人外周血浆细胞样树突状细胞中Toll样受体7的表达及其意义

作者简介: 刘梦洁(1992-),女,硕士研究生.

English Abstract

The expression of Toll-like receptor 7 in peripheral plasmacytoid dendritic cells in patients with systemic lupus erythematosus,and its significance

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目录