Wnt/β-catenin信号通路拮抗因子sFRP1在宫颈组织中的表达及其与HPV相关性研究

曹丽雯; 何玉; 欧玉荣

doi:10.13898/j.cnki.issn.1000-2200.2018.12.002

Wnt/β-catenin信号通路拮抗因子sFRP1在宫颈组织中的表达及其与HPV相关性研究

1. 蚌埠医学院第一附属医院妇产科, 安徽蚌埠 233004;
2. 蚌埠医学院第一附属医院病理科, 安徽蚌埠 233004

作者简介: 曹丽雯(1992-),女,硕士研究生.

中图分类号: R737.33

Study on the expression of Wnt/β-catenin signaling pathway antagonist sFRP1 in cervical tissue and its correlation with HPV infection

1. Department of Obstetrics and Gynecology, The First Affiliated Hospital of Bengbu Medical College, Bengbu Anhui 233004 China;
2. Department of Pathology, The First Affiliated Hospital of Bengbu Medical College, Bengbu Anhui 233004 China
CLC number: R737.33

摘要: 目的：研究Wnt信号通路中分泌型卷曲相关蛋白（sFRP1）和β-连环蛋白（β-catenin）在宫颈癌变组织中的表达及其临床意义，并探讨两者与HPV感染的相关情况。方法：收集宫颈鳞癌（SCC）60例、宫颈上皮内瘤变70例（LSIL 15例，HSIL 60例）和30例正常宫颈组织，采用免疫组织化学SP法检测各组织sFRP1和β-catenin蛋白的表达情况，同时应用杂交捕获（HC₂）技术行宫颈黏液脱落细胞HPV-DNA检测。结果：sFRP1在正常宫颈、宫颈上皮内瘤变、SCC的细胞膜表达是逐渐缺失的，β-catenin在上述组织的细胞质、细胞核中表达是逐渐增强的。不同病理分化程度与浸润深度的SCC组织sFRP1表达差异有统计学意义（P<0.05），不同FIGO分期与浸润深度的SCC组织β-catenin表达差异有统计学意义（P<0.05）。在SCC组织中，β-catenin蛋白和sFRP1蛋白表达呈负相关（P<0.05）；β-catenin蛋白与HPV感染呈正相关（P<0.01）；sFRP1蛋白表达与HPV感染呈负相关（P<0.05）。结论：sFRP-1、β-catenin蛋白表达及HPV感染联合检测对判断宫颈癌的发生、发展及预后有重要意义。
- 宫颈鳞状细胞癌 /
- Wnt信号通路 /
- 分泌型卷曲相关蛋白 /
- β-连环蛋白 /
- 人乳头瘤病毒
Abstract: Objective:To study the expression levels and clinical significance of secretory crimp(sFRP1) and β-catenin in Wnt signaling pathway,and its correlation with HPV infection.Methods:The expression levels of sFRP1 and β-catenin protein in 60 cervical squamous cell carcinoma(SCC) cases,75 cervical intraepithelial neoplasia cases(including 15 cases of LSIL and 60 cases of HSIL) and 30 normal cervical tissue cases were detected using immunohistochemical SP method,and the HPV-DNA of cervical mucus exfoliated cells was detected using the hybrid capture(HC₂) technology.Results:The expression levels of sFRP1 protein in cytomembrane of normal cervical tissue,cervical intraepithelial neoplasia and cervical squamous carcinoma gradually decreased,and the expression levels of β-catenin protein in cytoplasm and nucleus of normal cervical tissue,cervical intraepithelial neoplasia and cervical squamous carcinoma gradually increased.The differences of sFRP1 expression in SCC tissue with different pathological differentiation and infiltration depth were statistically significant(P<0.05),and the differences of β-catenin expression in SCC tissue with different FIGO stages and infiltration depth were statistically significant(P<0.05).In SCC tissue,the expression of β-catenin was negatively correlated with the expression of sFRP1(P<0.05),the expression of β-catenin was negatively correlated with HPV infection(P<0.01),and the expression of sFRP1 was negatively correlated with HPV infection(P<0.05).Conclusions:The combined detection of the expression of sFRP-1 and β-catenin,and HPV infection has important significance in judging the occurrence,development and prognosis of cervical cancer.
- cervical squamous cell carcinoma /
- Wnt signaling pathway /
- secretory crimp /
- β-catenin /
- human papilloma virus

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Wnt/β-catenin信号通路拮抗因子sFRP1在宫颈组织中的表达及其与HPV相关性研究

作者简介: 曹丽雯(1992-),女,硕士研究生.

Study on the expression of Wnt/β-catenin signaling pathway antagonist sFRP1 in cervical tissue and its correlation with HPV infection

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Wnt/β-catenin信号通路拮抗因子sFRP1在宫颈组织中的表达及其与HPV相关性研究

作者简介: 曹丽雯(1992-),女,硕士研究生.

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Study on the expression of Wnt/β-catenin signaling pathway antagonist sFRP1 in cervical tissue and its correlation with HPV infection

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Wnt/β-catenin信号通路拮抗因子sFRP1在宫颈组织中的表达及其与HPV相关性研究

作者简介: 曹丽雯(1992-),女,硕士研究生.

Study on the expression of Wnt/β-catenin signaling pathway antagonist sFRP1 in cervical tissue and its correlation with HPV infection

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Wnt/β-catenin信号通路拮抗因子sFRP1在宫颈组织中的表达及其与HPV相关性研究

作者简介: 曹丽雯(1992-),女,硕士研究生.

English Abstract

Study on the expression of Wnt/β-catenin signaling pathway antagonist sFRP1 in cervical tissue and its correlation with HPV infection

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目录