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近年,环状RNA为一种新出现的非编码RNA被人们重视,它们在多种肿瘤中有特异性表达,可以作为一种新的生物标志物对肿瘤进行诊断[1-2]。尽管环状RNA在多种疾病中起到关键作用,如卵巢癌、结直肠癌、特发性肺纤维化等[3],但在很多癌症中的表达机制和作用尚不清楚。本研究旨在探讨环状RNA circ_100290在结直肠癌中的表达及其临床意义,现作报道。
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qRT-PCR检测结果发现:人环状RNA circ_100290在结直肠癌组织中的相对表达量为3.15±0.14,高于人环状RNA circ_100290在对应结直肠癌旁组织中的相对表达量0.98±0.03(t=175.44,P < 0.01)。
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circ_100290表达量与临床病理特征的相关性 将结直肠癌组织中人环状RNA circ_100290表达量取中位数分为高表达组与低表达组,人环状RNA circ_100290高表达与淋巴结转移、远处转移和TNM分期有关(P < 0.05~P < 0.01),与性别、年龄、肿瘤大小、血管浸润和分化程度无关(见表 1)。
临床
病理特征n circ_100290表达量 χ2 P 低表达 高表达 年龄/岁 >60 59 30 29 0.30 >0.05 ≤60 75 37 38 性别 女 52 27 25 0.13 >0.05 男 82 40 42 肿瘤大小/cm >5 60 28 32 0.48 >0.05 ≤5 74 39 35 淋巴结转移 无 63 41 22 10.81 < 0.01 有 71 26 45 血管浸润 无 110 57 53 0.81 >0.05 有 24 10 14 远处转移 无 56 34 22 4.42 < 0.05 有 78 33 45 分化程度 高中分化 67 30 37 1.46 >0.05 低分化 67 37 30 TNM分期 Ⅰ~Ⅱ 80 45 35 3.89 < 0.05 Ⅲ~Ⅳ 54 21 33 表 1 结直肠癌组织人环状RNA circ_100290表达与临床病理特征的相关性
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在随访期间,134例结直肠癌病人中有39例死亡(25例来自人环状RNA circ_100290高表达组,14例来自人环状RNA circ_100290低表达组)。Kaplan-Meier分析结果发现,与人环状RNA circ_100290表达低的病人相比,人环状RNA circ_100290高表达的病人总生存的平均时间更短(P < 0.05)(见图 1)。
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单变量和多变量Cox比例风险回归模型分析结果均表明,人环状RNA circ_100290表达水平、淋巴结转移、远处转移和TNM分期与结直肠癌病人的总生存率相关(P < 0.05~P < 0.01)(见表 2)。
变量 单变量分析 多变量分析 HR 95%CI P HR 95%CI P 年龄 1.214 0.655~2.825 >0.05 1.316 0.592~2.661 >0.05 性别 1.304 0.805~2.135 >0.05 1.427 0.561~2.944 >0.05 肿瘤大小 1.814 0.528~3.234 >0.05 1.739 0.623~3.234 >0.05 淋巴结转移 3.251 1.541~5.734 < 0.01 2.958 1.268~4.884 < 0.05 血管浸润 1.645 0.815~2.845 >0.05 1.563 0.863~2.375 >0.05 远处转移 2.986 0.715~4.741 < 0.05 2.745 1.621~4.255 < 0.05 分化程度 2.974 0.654~4.665 >0.05 2.268 0.378~3.254 >0.05 TNM分期 2.384 1.323~4.356 < 0.05 2.698 1.521~3.985 < 0.05 circ_100290 3.553 1.474~6.841 < 0.05 3.298 1.531~5.515 < 0.05 表 2 Cox比例风险回归模型进行单变量和多变量回归分析
环状RNA circ_100290在结直肠癌中的表达及其临床意义
Expression of circ_100290 in colorectal cancer and its clinical significance
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摘要:
目的探讨环状RNA circ_100290在结直肠癌病人中的表达,及其与临床病理特征及预后的关系。 方法实时荧光定量聚合酶链反应(qRT-PCR)法检测结直肠癌及其癌旁正常组织中环状RNA circ_100290的表达量。分析环状RNA circ_100290的表达差异与临床资料及病例特征的关系。生存曲线分析环状RNA circ_100290的表达差异与临床预后的相关性。Cox比例风险回归模型进行单变量和多变量回归分析。 结果环状RNA circ_100290在结直肠癌组织中的相对表达水平高于癌旁肠正常组织(P < 0.01)。环状RNA circ_100290高表达与与淋巴结转移、远处转移、TNM分期有关(P < .05~P < 0.01)。生存曲线分析显示,与人环状RNA circ_100290表达低的病人相比,高表达的病人总生存的平均时间更短(P < 0.05);单变量与多变量Cox比例风险回归模型分析均显示,环状RNA circ_100290表达水平、淋巴结转移、远处转移和TNM分期与结肠癌病人的总生存率均有关系(P < 0.05~P < 0.01)。 结论环状RNA circ_100290高表达与结直肠癌转移和预后相关。因此,环状RNA circ_100290可以作为预测结肠癌病人预后的潜在生物标志物。 -
关键词:
- 结直肠肿瘤 /
- 环状RNA /
- circ_100290 /
- 预后
Abstract:ObjectiveTo investigate the expression level of circular RNA circ_100290 in colorectal cancer patients, and its correlation with clinicopathological features of colorectal cancer and prognosis. MethodsThe expression levels of circ_100290 in colorectal cancer tissue and normal tissue adjacent to cancer were detected using qRT-PCR.The relationship between circ_100290 expression level, and clinical data and case characteristics were analyzed.The correlation between circ_100290 expression level and clinical prognosis was analyzed using survival curve analysis.The Cox proportional hazard regression model was used for univariate and multivariate regression analysis. ResultsThe expression level of circ_100290 in colorectal cancer tissue was higher than that in normal tissue(P < 0.01), and the high expression of circ_100290 was related to the lymph node metastasis, distant metastasis and TNM stage(P < 0.05 to P < 0.01).The results of Kaplan-Meier survival analysis showed that the total mean survival time in patients with high circ_100290 expression was shorter than that in patients with low circ_100290 expression(P < 0.05).The results of univariate Cox proportional hazards regression model analysis indicated that the expression level of circ_100290, lymph node metastasis, distant metastasis and TNM stage were correlated with the overall survival rate of colorectal cancer patients(P < 0.05 to P < 0.01). ConclusionsThe high expression of circ_100290 is related to the colorectal cancer metastasis and prognosis.The circ_100290 may serve as a promising biomarker in predicting the prognosis of colorectal cancer. -
Key words:
- colorectal neoplasms /
- circular RNA /
- circ_100290 /
- prognosis
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表 1 结直肠癌组织人环状RNA circ_100290表达与临床病理特征的相关性
临床
病理特征n circ_100290表达量 χ2 P 低表达 高表达 年龄/岁 >60 59 30 29 0.30 >0.05 ≤60 75 37 38 性别 女 52 27 25 0.13 >0.05 男 82 40 42 肿瘤大小/cm >5 60 28 32 0.48 >0.05 ≤5 74 39 35 淋巴结转移 无 63 41 22 10.81 < 0.01 有 71 26 45 血管浸润 无 110 57 53 0.81 >0.05 有 24 10 14 远处转移 无 56 34 22 4.42 < 0.05 有 78 33 45 分化程度 高中分化 67 30 37 1.46 >0.05 低分化 67 37 30 TNM分期 Ⅰ~Ⅱ 80 45 35 3.89 < 0.05 Ⅲ~Ⅳ 54 21 33 表 2 Cox比例风险回归模型进行单变量和多变量回归分析
变量 单变量分析 多变量分析 HR 95%CI P HR 95%CI P 年龄 1.214 0.655~2.825 >0.05 1.316 0.592~2.661 >0.05 性别 1.304 0.805~2.135 >0.05 1.427 0.561~2.944 >0.05 肿瘤大小 1.814 0.528~3.234 >0.05 1.739 0.623~3.234 >0.05 淋巴结转移 3.251 1.541~5.734 < 0.01 2.958 1.268~4.884 < 0.05 血管浸润 1.645 0.815~2.845 >0.05 1.563 0.863~2.375 >0.05 远处转移 2.986 0.715~4.741 < 0.05 2.745 1.621~4.255 < 0.05 分化程度 2.974 0.654~4.665 >0.05 2.268 0.378~3.254 >0.05 TNM分期 2.384 1.323~4.356 < 0.05 2.698 1.521~3.985 < 0.05 circ_100290 3.553 1.474~6.841 < 0.05 3.298 1.531~5.515 < 0.05 -
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