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创伤性脑损伤(TBI)是现代社会一种常见身体损伤,多为意外摔倒、车祸伤、高处坠落等导致,伤后病人病情危急,预后不佳。通过深入研究,近来发现TBI后继发炎症性脑损伤是影响病人预后的重要因素[1-2]。尧小龙等[3]报道TBI后通过Toll样受体(TLRs)/ κB信号通路激活小胶质细胞释放炎性因子,启动炎性反应。TLR2在实验性脑出血模型中被证明是炎症性脑损伤的重要启动因子[4],但在TBI中是否也存在类似机制鲜有报道。因此,本文通过鼠脑损伤模型研究,阐明TLR2在脑损伤后的表达及作用,同时评价芝麻素的抗炎效果。
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3组小鼠的脑水含量差异有统计学意义(P<0.05),脑外伤组脑水含量高于假手术组和芝麻素治疗组(P<0.05)。3组小鼠组织TLR2蛋白表达差异有统计学意义(P<0.01),脑外伤组TLR2蛋白表达水平显著高于假手术组和芝麻素治疗组(P<0.01)(见表 1)。
分组 n 脑水含量/% TLR2蛋白量 假手术组 5 71.57±7.9 4.67±0.42 脑外伤组 5 82.16±4.6* 6.84±0.98** 芝麻素组 5 74.26±5.6# 5.41±0.17## F — 3.95 15.66 P — <0.05 <0.01 MS组内 — 38.310 0.389 q检验:与假手术组比较* P<0.05,**P<0.01;与脑外伤组比较#P<0.05,##P<0.01 表 1 3组小鼠脑水含量及脑组织TLR2蛋白表达比较(x±s)
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与假手术组相比,脑外伤组中IL-1β、IL-6和TNF-α mRNA的表达水平明显增高(P<0.05~P<0.01);而芝麻素处理组中脑组织中IL-1β、IL-6、TNF-α mRNA的表达水平则显著低于脑外伤组(P<0.05~P<0.01)(见表 2)。
分组 n IL-1β mRNA IL-6 mRNA TNF-α mRNA 假手术组 5 6.81±0.92 10.56±1.86 5.13±0.44 脑外伤组 5 9.32±1.06** 14.38±2.14* 8.89±0.57** 芝麻素组 5 7.49±0.79# 11.47±1.21# 6.49±0.36**## F — 9.74 6.28 83.89 P — <0.01 <0.01 <0.01 MS组内 — 0.865 3.168 0.216 q检验:与假手术组比较*P<0.05,**P<0.01;与脑外伤组比较#P<0.05,##P<0.01 表 2 3组小鼠脑组织IL-1β mRNA、IL-6 mRNA、TNF-α mRNA的表达比较(x±s)
芝麻素对Toll样受体2在实验性颅脑损伤中表达的影响
Effect of sesamin on expression of Toll-like receptor 2 in experimental brain injury
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摘要:
目的探讨芝麻素对Toll样受体2(TLR2)在实验性颅脑损伤中表达的影响。 方法采用自由落体法建立鼠颅脑损伤模型,将45只小鼠随机分为假手术组、颅脑损伤组、脑外伤后芝麻素治疗组。称量脑组织干湿重观察脑水肿的变化;用Western blotting方法检测各组小鼠脑组织中TLR2表达;采用实时荧光定量PCR检测脑损伤周围组织中炎症因子的表达水平。 结果脑外伤组小鼠损伤侧脑水含量、TLR2表达及炎症因子表达水平较假手术组显著升高(P < 0.05~P < 0.01);而芝麻素治疗组的损伤侧脑水含量、TLR2蛋白表达及炎性因子表达水平则低于脑外伤组(P < 0.05~P < 0.01)。 结论TBI后脑组织中TLR2表达增高及炎症反应增强,而芝麻素能够抑制炎症反应,进而起到保护脑组织进一步损伤。 Abstract:ObjectiveTo investigate the effect of sesamin on the expression of Toll-like receptor 2 (TLR2) in experimental brain injury. MethodsA model of craniocerebral injury was established using free fall method, and 45 mice were randomly divided into the sham surgery group, craniocerebral injury group and sesamin treatment group after brain trauma.The change of brain edema was observed by measuring the dry and wet weight of brain tissue.The expression level of TLR2 protein in brain tissue of mice was detected using Western blotting.The expression levels of inflammatory factors in tissues surrounding brain injury were detected using real time fluorescence quantitative PCR. ResultsThe levels of brain water, TLR2 expression and inflammatory factor in craniocerebral injury group were significantly higher than those in sham surgery group (P < 0.05 to P < 0.01).The levels of brain water, TLR2 protein expression and inflammatory factor expression in sesame treatment group were lower than those in craniocerebral injury group (P < 0.05 to P < 0.01). ConclusionsThe TLR2 expression and inflammatory response in traumatic brain injury brain tissue strengthen, and sesamin can inhibit inflammatory response and protect brain tissue from further injury. -
Key words:
- traumatic brain injury /
- Toll-like receptor 2 /
- signaling pathway /
- inflammatory response /
- sesamin
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表 1 3组小鼠脑水含量及脑组织TLR2蛋白表达比较(x±s)
分组 n 脑水含量/% TLR2蛋白量 假手术组 5 71.57±7.9 4.67±0.42 脑外伤组 5 82.16±4.6* 6.84±0.98** 芝麻素组 5 74.26±5.6# 5.41±0.17## F — 3.95 15.66 P — <0.05 <0.01 MS组内 — 38.310 0.389 q检验:与假手术组比较* P<0.05,**P<0.01;与脑外伤组比较#P<0.05,##P<0.01 表 2 3组小鼠脑组织IL-1β mRNA、IL-6 mRNA、TNF-α mRNA的表达比较(x±s)
分组 n IL-1β mRNA IL-6 mRNA TNF-α mRNA 假手术组 5 6.81±0.92 10.56±1.86 5.13±0.44 脑外伤组 5 9.32±1.06** 14.38±2.14* 8.89±0.57** 芝麻素组 5 7.49±0.79# 11.47±1.21# 6.49±0.36**## F — 9.74 6.28 83.89 P — <0.01 <0.01 <0.01 MS组内 — 0.865 3.168 0.216 q检验:与假手术组比较*P<0.05,**P<0.01;与脑外伤组比较#P<0.05,##P<0.01 -
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