• 中国科技论文统计源期刊
  • 中国科技核心期刊
  • 中国高校优秀期刊
  • 安徽省优秀科技期刊
Volume 39 Issue 6
Dec.  2014
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Effect of arsenic trioxide on the long chain noncoding HOTAIR expression in breast cancer cells

  • Corresponding author: YANG Qing-ling, yqlmimi@163.com
  • Received Date: 2014-02-04
  • Objective:To investigate the expression of long-chain non-coding HOX transcript antisense RNA(HOTAIR) in breast cancer tissues and cells,and effects of arsenic trioxide(As2O3) on its expression.Methods:The expressions of long-chain non-coding HOTAIR mRNA in breast carcer tissue and pericarcinomatous tissue were detected using real-time fluorescence quantitative PCR.Breast cancer cell lines with high expression HOTAIR was screened,the effect of As2O3 on the expression of HOTAIR mRNA was detected.The expressions of HOTAIR between breast cancer tissue,pericarcinomatous tissue and breast cancer cell lines were compared.The HOTAIR expression in MCF-7 cells treated with As2O3 was detected.Results:The expression quantity of HOTAIR mRNA in breast cancer was 0.011?0.005,no expression of HOTAIR was found in pericarcinomatous tissue.The HOTAIR mRNA expression was not found in SKBR-3 and MAD-MB-231 cells.The high expression of HOTAIR mRNA(1?0.236) was detected in MCF-7 cells.The expression level of HOTAIR mRNA in MCF-7 cells treated with As2O3 was significantly lower than that in blank group(PConclusions:The HOTAIR expression is associated with the incidence of breast cancer,and As2O3 can inhibit its expression.
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    [5] Gupta RA,Shah N,Wang KC,et al. Long non-coding RNA HOTAIR reprograms chromatin state to promote cancer metastasis[J]. Nature,2010,464( 7291) : 1071-1076
    [6] Rinn JL,Kertesz M,Wang JK,et al.Functional demarcation of active and silent chromatin domains in human HOX loci by noncoding RNAs[J].Cell,2007,129(7):1311-1323.
    [7] Gupta RA,Shah N,Wang KC,et al.Long non-coding RNA HOTAIR reprograms chromatin state to promote cancer metastasis[J].Nature,2010,464(7291):1071-1076. [8] Yang Z,Zhou L,Wu LM,et al.Overexpression of long non-coding RNA HOTAIR predicts tumor recurrence in hepatocellular carcinoma patients following liver transplantation[J].Ann Surg Oncol,2011,18(5):1243-1250. [9] Srensen KP,Thomassen M,Tan Q,et al.Long non-coding RNA HOTAIR is an independent prognostic marker of metastasis in estrogen receptor-positive primary breast cancer[J].Breast Cancer Res Treat,2013,142(3):529-536. [10] Geng YJ,Xie S,Li Q,et al.Large intervening non-coding RNA HOTAIR is associated with hepatocellular carcinoma progression[J].J Int Med Res,2011,39(6):2119-2128. [11] Kogo R,Shimamura T,Mimori K,et al.Long noncoding RNA HOTAIR regulates polycomb-dependent chromatin modification and is associated with poor prognosis in colorectal cancers[J].Cancer Res,2011,71(20):6320-6326. [12] Nie Y,Liu X,Qu S,et al.Long non-coding RNA HOTAIR is an independent prognostic marker for nasopharyngeal carcinoma progression and survival[J].Cancer Sci,2013,104(4):458-464. [13] Tsai MC,Manor O,Wan Y,et al.Long noncoding RNA as modular scaffold of histone modification complexes[J].Science,2010,329(5992):689-693. [14] Marchese FP,Huarte M.Long non-coding RNAs and chromatin modifiers:their place in the epigenetic code[J].Epigenetics,2013,9(1):21-26. [15] Tano K,Akimitsu N.Long non-coding RNAs cancer progression[J].Frontiers in Genetics,2011,3:219. [16] Zhang JX,Han L,Bao ZS,et al.HOTAIR,a cell cycle–associated long noncoding RNA and a strong predictor of survival,is preferentially expressed in classical and mesenchymal glioma[J].Neuro Oncol,2013,15(12):1595-1603. [17] Shen ZX,Chen GQ,Ni JH,et al.Use of arsenic trioxide in the treatment of acute promyelocytic leukemia(APL):ⅡClinical efficacy and pharmacokinetics in relapsed patients[J].Blood,1997,89(9):3354-3360. [18] Tingting R,Wei G,Changliang P,et al.Arsenic trioxide inhibits osteosarcoma cell invasiveness via MAPK signaling pathway[J].Cancer Biol Ther,2010,10(3):251-257.
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Effect of arsenic trioxide on the long chain noncoding HOTAIR expression in breast cancer cells

    Corresponding author: YANG Qing-ling, yqlmimi@163.com
  • 1. 1. Department of Biochemistry & Molecular Biology;
  • 2. 2. Clinical Testing and Diagnose Experimental Center, Bengbu Medical College, Bengbu Anhui 233030, China

Abstract: Objective:To investigate the expression of long-chain non-coding HOX transcript antisense RNA(HOTAIR) in breast cancer tissues and cells,and effects of arsenic trioxide(As2O3) on its expression.Methods:The expressions of long-chain non-coding HOTAIR mRNA in breast carcer tissue and pericarcinomatous tissue were detected using real-time fluorescence quantitative PCR.Breast cancer cell lines with high expression HOTAIR was screened,the effect of As2O3 on the expression of HOTAIR mRNA was detected.The expressions of HOTAIR between breast cancer tissue,pericarcinomatous tissue and breast cancer cell lines were compared.The HOTAIR expression in MCF-7 cells treated with As2O3 was detected.Results:The expression quantity of HOTAIR mRNA in breast cancer was 0.011?0.005,no expression of HOTAIR was found in pericarcinomatous tissue.The HOTAIR mRNA expression was not found in SKBR-3 and MAD-MB-231 cells.The high expression of HOTAIR mRNA(1?0.236) was detected in MCF-7 cells.The expression level of HOTAIR mRNA in MCF-7 cells treated with As2O3 was significantly lower than that in blank group(PConclusions:The HOTAIR expression is associated with the incidence of breast cancer,and As2O3 can inhibit its expression.

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