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2014 Vol. 39, No. 6
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2014, 38(6): 701-704.
Abstract:
Objective:To investigate the effects of fasudil on the acute lung injury(ALI) in rats induced by lipopolysaccharide(LPS) through the RhoA/Rho kinase pathway.Methods:Eighteen wistar rats were randomly divided into control group,LPS group and fasudil(FAS) group(6 rats each group).The ALI model was established by intraperitoneal injection of LPS 1 mg/kg for 16 hours,and then endotracheal instillation of LPS 3 mg/kg.The fasudil 10 mg/kg was intraperitoneally injected into FAS group at 30 min before intraperitoneal injection of LPS and 1 hour after endotracheal instillation of LPS.The rats were sacrificed after 3 hours.The morphological changes of lung tissue in 3 groups were observed by HE staining,the ratio of wet-to-dry weight of lung,myeloperoxidase activity,malondialdehyde content in 3 groups were measured,and the ROCK2(Rho kinase 2) mRNA and protein expressions in 3 groups were detected by reverse transcription-polymerase chain reaction and Western blot,respectively.Results:Compared with the control group,the lung histopathology change in LPS group was obvious,and the lung histopathology change in FAS group was significantly gentler than that in LPS group.The ratio of wet-to-dry weight of lung,myeloperoxidase activity,malondialdehyde content in LPS group were significantly higher than those in control group(P0.01),but the indexes in FAS and LPS group were decreased to varying degrees(P0.05 to P0.01).Compared with the control group,the ROCK2 mRNA expression of lung in LPS group was increased(P0.01),but the difference of which had no statistical significance between FAS and LPS group(P0.05).Compared with the control group,the ROCK2 protein expression in LPS group was significantly increased(P0.01).Compared with the LPS group,the ROCK2 protein expression in FAS group was significantly decreased(P0.05).Conclusions:Fasudil can reduce the acute lung injury in rats induced by lipopolysaccharide through the RhoA/Rho kinase signal pathway.
Objective:To investigate the effects of fasudil on the acute lung injury(ALI) in rats induced by lipopolysaccharide(LPS) through the RhoA/Rho kinase pathway.Methods:Eighteen wistar rats were randomly divided into control group,LPS group and fasudil(FAS) group(6 rats each group).The ALI model was established by intraperitoneal injection of LPS 1 mg/kg for 16 hours,and then endotracheal instillation of LPS 3 mg/kg.The fasudil 10 mg/kg was intraperitoneally injected into FAS group at 30 min before intraperitoneal injection of LPS and 1 hour after endotracheal instillation of LPS.The rats were sacrificed after 3 hours.The morphological changes of lung tissue in 3 groups were observed by HE staining,the ratio of wet-to-dry weight of lung,myeloperoxidase activity,malondialdehyde content in 3 groups were measured,and the ROCK2(Rho kinase 2) mRNA and protein expressions in 3 groups were detected by reverse transcription-polymerase chain reaction and Western blot,respectively.Results:Compared with the control group,the lung histopathology change in LPS group was obvious,and the lung histopathology change in FAS group was significantly gentler than that in LPS group.The ratio of wet-to-dry weight of lung,myeloperoxidase activity,malondialdehyde content in LPS group were significantly higher than those in control group(P0.01),but the indexes in FAS and LPS group were decreased to varying degrees(P0.05 to P0.01).Compared with the control group,the ROCK2 mRNA expression of lung in LPS group was increased(P0.01),but the difference of which had no statistical significance between FAS and LPS group(P0.05).Compared with the control group,the ROCK2 protein expression in LPS group was significantly increased(P0.01).Compared with the LPS group,the ROCK2 protein expression in FAS group was significantly decreased(P0.05).Conclusions:Fasudil can reduce the acute lung injury in rats induced by lipopolysaccharide through the RhoA/Rho kinase signal pathway.
2014, 38(6): 705-710.
Abstract:
Objective:To investigate the effects of Helicobacter pylori(Hp) on autophagy and apoptosis of gastric epithelial cells.Methods:Human gastric epithelial cell line GES-1(GES-1) were infected by Hp in vitro culture,the intervention groups of Hp-infected GES-1 were treated with autophagy inducer-rapamycin(RAPA) and autophagy inhibitor-3-methyladenine(3-MA),and uninfected cell was set as the blank control group.Cells were collected at 2,4,6,12 and 24 h after infection.The effects of Hp infection on autophagy and apoptosis of GES-1 were observed by Western Blotting,fluorescent staining,transmission electron microscopy,flow cytometry and intracellular bacterial survival counting.Results:Autophagy marker protein microtubule associated protein 1 light chain 3-Ⅱ expression and cellular autophagy fluorescent particles were found at 2 h after treatment,the typical autophagic vacuoles with double or multilayer membrane in the cytoplasm were observed by transmission electron microscopy.Autophagy of infected cells peaked at 4 h,gradually decreased and disappeared.Autophagy of RAPA-treated cells infected by Hp further enhanced,while the apoptotic rate and intracellular bacterial survival were lower than those in RAPA-untreated infected cells(PPPConclusions:Hp can induce the autophagy in gastric epithelial cells in the early infection.The autophagy inducer-RAPA and autophagy inhibitor-3-MA can promote or reduce the autophagy in Hp-infected cells,which can lessen or enhance injury of infected cells.
Objective:To investigate the effects of Helicobacter pylori(Hp) on autophagy and apoptosis of gastric epithelial cells.Methods:Human gastric epithelial cell line GES-1(GES-1) were infected by Hp in vitro culture,the intervention groups of Hp-infected GES-1 were treated with autophagy inducer-rapamycin(RAPA) and autophagy inhibitor-3-methyladenine(3-MA),and uninfected cell was set as the blank control group.Cells were collected at 2,4,6,12 and 24 h after infection.The effects of Hp infection on autophagy and apoptosis of GES-1 were observed by Western Blotting,fluorescent staining,transmission electron microscopy,flow cytometry and intracellular bacterial survival counting.Results:Autophagy marker protein microtubule associated protein 1 light chain 3-Ⅱ expression and cellular autophagy fluorescent particles were found at 2 h after treatment,the typical autophagic vacuoles with double or multilayer membrane in the cytoplasm were observed by transmission electron microscopy.Autophagy of infected cells peaked at 4 h,gradually decreased and disappeared.Autophagy of RAPA-treated cells infected by Hp further enhanced,while the apoptotic rate and intracellular bacterial survival were lower than those in RAPA-untreated infected cells(PPPConclusions:Hp can induce the autophagy in gastric epithelial cells in the early infection.The autophagy inducer-RAPA and autophagy inhibitor-3-MA can promote or reduce the autophagy in Hp-infected cells,which can lessen or enhance injury of infected cells.
2014, 38(6): 711-713,717.
Abstract:
Objective:To investigate the expression of long-chain non-coding HOX transcript antisense RNA(HOTAIR) in breast cancer tissues and cells,and effects of arsenic trioxide(As2O3) on its expression.Methods:The expressions of long-chain non-coding HOTAIR mRNA in breast carcer tissue and pericarcinomatous tissue were detected using real-time fluorescence quantitative PCR.Breast cancer cell lines with high expression HOTAIR was screened,the effect of As2O3 on the expression of HOTAIR mRNA was detected.The expressions of HOTAIR between breast cancer tissue,pericarcinomatous tissue and breast cancer cell lines were compared.The HOTAIR expression in MCF-7 cells treated with As2O3 was detected.Results:The expression quantity of HOTAIR mRNA in breast cancer was 0.011?0.005,no expression of HOTAIR was found in pericarcinomatous tissue.The HOTAIR mRNA expression was not found in SKBR-3 and MAD-MB-231 cells.The high expression of HOTAIR mRNA(1?0.236) was detected in MCF-7 cells.The expression level of HOTAIR mRNA in MCF-7 cells treated with As2O3 was significantly lower than that in blank group(PConclusions:The HOTAIR expression is associated with the incidence of breast cancer,and As2O3 can inhibit its expression.
Objective:To investigate the expression of long-chain non-coding HOX transcript antisense RNA(HOTAIR) in breast cancer tissues and cells,and effects of arsenic trioxide(As2O3) on its expression.Methods:The expressions of long-chain non-coding HOTAIR mRNA in breast carcer tissue and pericarcinomatous tissue were detected using real-time fluorescence quantitative PCR.Breast cancer cell lines with high expression HOTAIR was screened,the effect of As2O3 on the expression of HOTAIR mRNA was detected.The expressions of HOTAIR between breast cancer tissue,pericarcinomatous tissue and breast cancer cell lines were compared.The HOTAIR expression in MCF-7 cells treated with As2O3 was detected.Results:The expression quantity of HOTAIR mRNA in breast cancer was 0.011?0.005,no expression of HOTAIR was found in pericarcinomatous tissue.The HOTAIR mRNA expression was not found in SKBR-3 and MAD-MB-231 cells.The high expression of HOTAIR mRNA(1?0.236) was detected in MCF-7 cells.The expression level of HOTAIR mRNA in MCF-7 cells treated with As2O3 was significantly lower than that in blank group(PConclusions:The HOTAIR expression is associated with the incidence of breast cancer,and As2O3 can inhibit its expression.
2014, 38(6): 714-717.
Abstract:
Objective:To evaluate the biocompatibility of Bi0.5 Na0.5TiO3-based(BNT) lead-free piezoceramics material in vitro.Methods:Human osteoblast-like cell lines were cultured with BNT，(LixNayK1-x-y)NbO3-based,polymethylmethacrylate，and blank control groups，the morphology and proliferation function of the osteoblast-like cells were observed by scanning electron microscopy and 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.Results:The osteoblast-like cell lines adhered,grew and proliferated normally and perfectly on the surface of the material.Compared with the (LixNayK1-x-y)NbO3-based，polymethylmethacrylate and blank control groups,BNT showed a higher optical density at 1,3,5,7,9 days(PConclusions:The new piezoelectric ceramic seems to possess the good biocompatibility in vitro，it may be a new type of biological material.
Objective:To evaluate the biocompatibility of Bi0.5 Na0.5TiO3-based(BNT) lead-free piezoceramics material in vitro.Methods:Human osteoblast-like cell lines were cultured with BNT，(LixNayK1-x-y)NbO3-based,polymethylmethacrylate，and blank control groups，the morphology and proliferation function of the osteoblast-like cells were observed by scanning electron microscopy and 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.Results:The osteoblast-like cell lines adhered,grew and proliferated normally and perfectly on the surface of the material.Compared with the (LixNayK1-x-y)NbO3-based，polymethylmethacrylate and blank control groups,BNT showed a higher optical density at 1,3,5,7,9 days(PConclusions:The new piezoelectric ceramic seems to possess the good biocompatibility in vitro，it may be a new type of biological material.
2014, 38(6): 718-720,723.
Abstract:
Objective:To detect the expression of the Dickkopf-1(DKK-1) in pancreas space-occupying lesions and its value in the diagnosis and differential diagnosis of pancreatic cancer.Methods:Sixty-three paraffin specimens of pancreas space-occupying lesions were collected,the expressions of DKK-1 in pancreatic cancer tissue and pancreatitis tissue were detected using immunohistochemical method.The blood CA19-9 level was measured by automatic labeling immunoluminescence analyzer.The relationships between DKK-1 and clinically pathological characteristics of patients and blood CA19-9 were analyzed.Results:The positive expression rate of DKK-1 in pancreatic cancer tissue(68.2%) was significantly higher than that in benign pancreatic lesions(5.3%)(PPPConclusions:The expression of DKK-1 in pancreatic cancer tissue is significantly higher than that in benign pancreatic lesion,which plays an important role in the formation and growth of pancreatic tumor.The detection of DKK-1 is helpful to the diagnosis and prognosis assessment of pancreatic cancer.
Objective:To detect the expression of the Dickkopf-1(DKK-1) in pancreas space-occupying lesions and its value in the diagnosis and differential diagnosis of pancreatic cancer.Methods:Sixty-three paraffin specimens of pancreas space-occupying lesions were collected,the expressions of DKK-1 in pancreatic cancer tissue and pancreatitis tissue were detected using immunohistochemical method.The blood CA19-9 level was measured by automatic labeling immunoluminescence analyzer.The relationships between DKK-1 and clinically pathological characteristics of patients and blood CA19-9 were analyzed.Results:The positive expression rate of DKK-1 in pancreatic cancer tissue(68.2%) was significantly higher than that in benign pancreatic lesions(5.3%)(PPPConclusions:The expression of DKK-1 in pancreatic cancer tissue is significantly higher than that in benign pancreatic lesion,which plays an important role in the formation and growth of pancreatic tumor.The detection of DKK-1 is helpful to the diagnosis and prognosis assessment of pancreatic cancer.
2014, 38(6): 721-723.
Abstract:
Objective:To investigate the curative effects and safety of amiodarone in the treatment of acute myocardial infarction elderly patients complicated with ventricular arrhythmia.Methods:One hundred and ten acute myocardial infarction elderly patients complicated with ventricular arrhythmia were divided into treatment group and control group.The treatment group(56 cases)and control group(54 cases) were treated with 150 to 300 mg of amiodarone by intravenous injection,then intravenous drip with 0.5 to 1.5 mg/min and 50 to 100 mg of lidocaine by intravenous injection,then intravenous drip with 1 to 4 mg/min,respectively.The number of ventricular arrhythmia,PR interval,QRS wave time,correct QT and adverse reactions in two groups were observed at 24 hours after treatment.Results:The total effective rate of the treatment group(94.64%) was higher than that of the control group(83.33%)(PPP>0.05).Conclusions:The effects of amiodarone in the treatment of acute myocardial infarction elderly patients complicated with ventricular arrhythmia are good,and no obvious added side effects are found.Amiodarone can be used as a preferred drug in treating acute myocardial infarction elderly patients complicated with ventricular arrhythmia.
Objective:To investigate the curative effects and safety of amiodarone in the treatment of acute myocardial infarction elderly patients complicated with ventricular arrhythmia.Methods:One hundred and ten acute myocardial infarction elderly patients complicated with ventricular arrhythmia were divided into treatment group and control group.The treatment group(56 cases)and control group(54 cases) were treated with 150 to 300 mg of amiodarone by intravenous injection,then intravenous drip with 0.5 to 1.5 mg/min and 50 to 100 mg of lidocaine by intravenous injection,then intravenous drip with 1 to 4 mg/min,respectively.The number of ventricular arrhythmia,PR interval,QRS wave time,correct QT and adverse reactions in two groups were observed at 24 hours after treatment.Results:The total effective rate of the treatment group(94.64%) was higher than that of the control group(83.33%)(PPP>0.05).Conclusions:The effects of amiodarone in the treatment of acute myocardial infarction elderly patients complicated with ventricular arrhythmia are good,and no obvious added side effects are found.Amiodarone can be used as a preferred drug in treating acute myocardial infarction elderly patients complicated with ventricular arrhythmia.
2014, 38(6): 724-725.
Abstract:
Objective:To explore the effects of the external removal of inferior turbinate in the correction of nasal septum deviation.Methods:Fifty-five patients with nasal septum deviation complicated with inferior turbinate hypertrophy were randomly divided into treatment group(33 cases) and nasal septum correction group(22 cases).The treatment group and nasal septum correction group were treated with the correction of nasal septum deviation combined with external removal of inferior turbinate and correction of nasal septum deviation,respectively.Results:All patients were followed up for more than 1 year.Amony the treatment group,25 patients were cured,8 patients were effective,the cure and improvement rates were 75.8% and 100.0%,respectively.Among the nasal septum correction group,7 patients were cured,11 patients were effective,and 4 patients were ineffective,the cure and improvement rates were 31.8% and 81.8%,respectively.The difference between the two groups had statistical significance(PConclusions:The correction of nasal septum deviation combined with external removal of inferior turbinate is little tissue damage,good effect,safety,consistent with the functional treatment,which is worth popularizing.
Objective:To explore the effects of the external removal of inferior turbinate in the correction of nasal septum deviation.Methods:Fifty-five patients with nasal septum deviation complicated with inferior turbinate hypertrophy were randomly divided into treatment group(33 cases) and nasal septum correction group(22 cases).The treatment group and nasal septum correction group were treated with the correction of nasal septum deviation combined with external removal of inferior turbinate and correction of nasal septum deviation,respectively.Results:All patients were followed up for more than 1 year.Amony the treatment group,25 patients were cured,8 patients were effective,the cure and improvement rates were 75.8% and 100.0%,respectively.Among the nasal septum correction group,7 patients were cured,11 patients were effective,and 4 patients were ineffective,the cure and improvement rates were 31.8% and 81.8%,respectively.The difference between the two groups had statistical significance(PConclusions:The correction of nasal septum deviation combined with external removal of inferior turbinate is little tissue damage,good effect,safety,consistent with the functional treatment,which is worth popularizing.
2014, 38(6): 726-729.
Abstract:
Objective:To investigate the clinical features and treatment of skin Fusarium infection.Methods:Sixteen cases of skin fusariun infections reported in China from 2001 to 2003 were collected and analyzed.Results:There were 10 cases of Fusarium solani infection,3 cases of Fusarium moniliforme infection,1 case of Fusarium oxysporum infection and 2 cases of unknown species in this group.Skin ulcer occurred in 12 cases,skin nodules in 3 cases,and skin ulcer and nodules in 1 case.One case presented disseminated Fusarium infection,2 cases the basic disease of acute leukemia and 1 case was complicated with squamouscell carcinoma.Fungal culture was proved positive in 16 cases;fungal component was detected by direct microscopy in 11 cases and by pathological examination in 7 cases.11 cases were treated with systemicantifungal therapy,and 8 of them were improved or cured.Conclusions:Fusarium solani is most commonly seen in Fusarium infection,presenting the symptoms of skin lesion,ulcer or nodular changes in general.Fungal culture,direct microscopic examination and histopathology examination are helpful to the diagnosis.There is no unified treatment protocol for Fusarium infection up to now.
Objective:To investigate the clinical features and treatment of skin Fusarium infection.Methods:Sixteen cases of skin fusariun infections reported in China from 2001 to 2003 were collected and analyzed.Results:There were 10 cases of Fusarium solani infection,3 cases of Fusarium moniliforme infection,1 case of Fusarium oxysporum infection and 2 cases of unknown species in this group.Skin ulcer occurred in 12 cases,skin nodules in 3 cases,and skin ulcer and nodules in 1 case.One case presented disseminated Fusarium infection,2 cases the basic disease of acute leukemia and 1 case was complicated with squamouscell carcinoma.Fungal culture was proved positive in 16 cases;fungal component was detected by direct microscopy in 11 cases and by pathological examination in 7 cases.11 cases were treated with systemicantifungal therapy,and 8 of them were improved or cured.Conclusions:Fusarium solani is most commonly seen in Fusarium infection,presenting the symptoms of skin lesion,ulcer or nodular changes in general.Fungal culture,direct microscopic examination and histopathology examination are helpful to the diagnosis.There is no unified treatment protocol for Fusarium infection up to now.
2014, 38(6): 729-731.
Abstract:
Objective:To investigate the effect of Jiangzhuoquyu decotion on the hemorheology and cell adhesion molecules levels in patients with carotid artery atherosclerosis.Methods:Sixty patients with carotid artery atherosclerosis were randomly divided into control group and therapy group.Patients in the control group were administered with simvastatin only,while patients in the therapy group were administered with Jiangzhuoquyu decotion and simvastatin,the total course of the treatment was 8 weeks.The hemorheology and plasma levels of soluble intercellular adhesion molecule-1(sICAM-1),soluble vascular cell adhesion molecule-1(sVCAM-1) and hypersensitive C reactive protein(hs-CRP) in two groups were measured.Results:Compared with the control group and before treatment in the therapy group,the hemorheology indexes and plasma levels of sICAM-1,sVCAM-1 and hs-CRP in therapy group were decreased significantly after treatment(PConclusions:Jiangzhuoquyu decotion can improve the hemorheology,and decrease the levels of sICAM-1,sVCAM-1 and hs-CRP in patients with carotid artery atherosclerosis.
Objective:To investigate the effect of Jiangzhuoquyu decotion on the hemorheology and cell adhesion molecules levels in patients with carotid artery atherosclerosis.Methods:Sixty patients with carotid artery atherosclerosis were randomly divided into control group and therapy group.Patients in the control group were administered with simvastatin only,while patients in the therapy group were administered with Jiangzhuoquyu decotion and simvastatin,the total course of the treatment was 8 weeks.The hemorheology and plasma levels of soluble intercellular adhesion molecule-1(sICAM-1),soluble vascular cell adhesion molecule-1(sVCAM-1) and hypersensitive C reactive protein(hs-CRP) in two groups were measured.Results:Compared with the control group and before treatment in the therapy group,the hemorheology indexes and plasma levels of sICAM-1,sVCAM-1 and hs-CRP in therapy group were decreased significantly after treatment(PConclusions:Jiangzhuoquyu decotion can improve the hemorheology,and decrease the levels of sICAM-1,sVCAM-1 and hs-CRP in patients with carotid artery atherosclerosis.
2014, 38(6): 732-734.
Abstract:
Objective:To explore the effects of fiber bronchoscope(FOB) and Lightwand wand on the hemodynamics of patients with difficult tracheal intubation through the mouth.Methods:Fifty patients with difficult airway treated with surgery through tracheal anesthesia were randomly divided into FOB group(F group) and Light wand group(L group).The systolic pressure,diastolic blood pressure,heart rate,pulse oxygen saturation in two groups were observed before anesthesia induction(T0),before intubation(T1),endotracheal intubation(T2),and at 1 minute(T3),3 minutes(T4) and 5 minutes(T5) after intubation,and the total time of endotracheal intubation,successful intubation cases and postoperative complications in two groups were recorded.Results:The endotracheal intubation success rate in F group and L group were 100.0% and 92.0%,respectively,the difference of which had no statistical significance(P>0.05).The differences of the incidences of postoperative voice hoarse and sore throat between two groups had no statistical significance(P>0.05).The total time of endotracheal intubation in F group was significantly less than that in L group(P2 and T3(PPConclusions:Compared with Lightwand,the effect of FOB on hemodynamics is smaller.Both FOB and Lightwand are effective methods to solve the difficult tracheal intubation.
Objective:To explore the effects of fiber bronchoscope(FOB) and Lightwand wand on the hemodynamics of patients with difficult tracheal intubation through the mouth.Methods:Fifty patients with difficult airway treated with surgery through tracheal anesthesia were randomly divided into FOB group(F group) and Light wand group(L group).The systolic pressure,diastolic blood pressure,heart rate,pulse oxygen saturation in two groups were observed before anesthesia induction(T0),before intubation(T1),endotracheal intubation(T2),and at 1 minute(T3),3 minutes(T4) and 5 minutes(T5) after intubation,and the total time of endotracheal intubation,successful intubation cases and postoperative complications in two groups were recorded.Results:The endotracheal intubation success rate in F group and L group were 100.0% and 92.0%,respectively,the difference of which had no statistical significance(P>0.05).The differences of the incidences of postoperative voice hoarse and sore throat between two groups had no statistical significance(P>0.05).The total time of endotracheal intubation in F group was significantly less than that in L group(P2 and T3(PPConclusions:Compared with Lightwand,the effect of FOB on hemodynamics is smaller.Both FOB and Lightwand are effective methods to solve the difficult tracheal intubation.
2014, 38(6): 735-738.
Abstract:
Objective:To compare the clinical value of the artificial vaginoplasty with pudendal-thigh skin flap transplantation and modified Vechietti vaginoplasty through Retzius gap,and explore the latter puncture path and safety.Methods:Eighteen patients with congenital absence of uterus and vagina were treated with vaginoplasty using pudendal-thigh skin flap transplantation(skin flap transplantation group,9 cases) and modified Vechietti vaginoplasty through Retzius gap(modified group,9 cases).The operation time,blood loss,hospital stay,complications and postoperative effects between two groups were compared.Results:All operations were successful.In skin flap transplantation group,except for 1 case lost following-up,8 cases were followed up for 3 months to 2 years.Mild vulvae shape change and 1 case with artificial vagina secondary infection in skin flap transplantation group were found.The modified group were followed up for 4 months to 2.5 years,no vulvae shape change and complications were found.The appropriate width and depth of vagina,lubricated and elastic mucosa and harmonious sexual life in two groups were confirmed.Compared with the skin flap transplantation group,the shorter operation time,less blood loss and shorter hospital stay were found in modified group,the differences of two groups were statistical significance(PConclusions:The modified Vechietti vaginoplasty through Retzius gap is minimally invasive,simple and safe,which is worth clinical promotion.
Objective:To compare the clinical value of the artificial vaginoplasty with pudendal-thigh skin flap transplantation and modified Vechietti vaginoplasty through Retzius gap,and explore the latter puncture path and safety.Methods:Eighteen patients with congenital absence of uterus and vagina were treated with vaginoplasty using pudendal-thigh skin flap transplantation(skin flap transplantation group,9 cases) and modified Vechietti vaginoplasty through Retzius gap(modified group,9 cases).The operation time,blood loss,hospital stay,complications and postoperative effects between two groups were compared.Results:All operations were successful.In skin flap transplantation group,except for 1 case lost following-up,8 cases were followed up for 3 months to 2 years.Mild vulvae shape change and 1 case with artificial vagina secondary infection in skin flap transplantation group were found.The modified group were followed up for 4 months to 2.5 years,no vulvae shape change and complications were found.The appropriate width and depth of vagina,lubricated and elastic mucosa and harmonious sexual life in two groups were confirmed.Compared with the skin flap transplantation group,the shorter operation time,less blood loss and shorter hospital stay were found in modified group,the differences of two groups were statistical significance(PConclusions:The modified Vechietti vaginoplasty through Retzius gap is minimally invasive,simple and safe,which is worth clinical promotion.
2014, 38(6): 738-740.
Abstract:
Objective:To study the morphological characteristics,diagnosis and differential diagnosis of fetus in fetu(FIF).Methods:Observed the histopathology of 2 cases of FIF,and the combination of literature review.Results:Morphological appearance of the mass has human organs,X-ray has vertebrae,vertebral axis section has formed cartilage and bone tissue,microscopic examination has the more mature differentiated normal human tissue.Conclusions:FIF is a mass which includes vertebral axis and the other organs or limbs;difficulty of diagnosis is to identify vertebral axis;mainly identification is teratoma.
Objective:To study the morphological characteristics,diagnosis and differential diagnosis of fetus in fetu(FIF).Methods:Observed the histopathology of 2 cases of FIF,and the combination of literature review.Results:Morphological appearance of the mass has human organs,X-ray has vertebrae,vertebral axis section has formed cartilage and bone tissue,microscopic examination has the more mature differentiated normal human tissue.Conclusions:FIF is a mass which includes vertebral axis and the other organs or limbs;difficulty of diagnosis is to identify vertebral axis;mainly identification is teratoma.
2014, 38(6): 741-744,748.
Abstract:
Objective:To observe the clinical efficacy and bone cell growth of porous tricalcium phosphate in the treatment of calcaneal fracture.Methods:Eighty-five patients with calcaneal fractures were treated with steel plate internal fixation under traditional incision(57 cases) and cannulated screw internal fixation under small incision(28 cases).Seventy-five patients were treated with tricalcium phosphate bone graft,the clinical efficacy of which was observed.The internal fixation was removed after fracture healing.The bone specimens from the original graft area were harvested,the histological feature of which was observed,and the growth of bone cells was analyzed.Results:The clinical effects of patients treated with steel plate internal fixation under traditional incision and internal fixation with bone graft under small incision were significantly better than that in patients treated with internal fixation without bone graft under small incision(PConclusions:Porous tricalcium phosphate has good bio-compatibility,biomechanical strength and bone conduction effect,the biological degradation effect of which is synchronized with bone tissue reconstruction.The new bone tissue after fracture healing is similar to the normal bone.The growth of new bone is good,which is similar to the original bone tissue.
Objective:To observe the clinical efficacy and bone cell growth of porous tricalcium phosphate in the treatment of calcaneal fracture.Methods:Eighty-five patients with calcaneal fractures were treated with steel plate internal fixation under traditional incision(57 cases) and cannulated screw internal fixation under small incision(28 cases).Seventy-five patients were treated with tricalcium phosphate bone graft,the clinical efficacy of which was observed.The internal fixation was removed after fracture healing.The bone specimens from the original graft area were harvested,the histological feature of which was observed,and the growth of bone cells was analyzed.Results:The clinical effects of patients treated with steel plate internal fixation under traditional incision and internal fixation with bone graft under small incision were significantly better than that in patients treated with internal fixation without bone graft under small incision(PConclusions:Porous tricalcium phosphate has good bio-compatibility,biomechanical strength and bone conduction effect,the biological degradation effect of which is synchronized with bone tissue reconstruction.The new bone tissue after fracture healing is similar to the normal bone.The growth of new bone is good,which is similar to the original bone tissue.
2014, 38(6): 745-748.
Abstract:
Objective:To investigate the serum levels of estradiol and testosterone in patients with hypertensive intracerebral hemorrhage and their relations with illness severity and prognosis.Methods:The serum levels of estradiol and testosterone in 168 patients with hypertensive intracerebral hemorrhage(cerebral hemorrhage group) and 60 healthy people(control group) were determined.All patients were scored by U.S.National Institutes of Health Stroke Scale,and compared in two groups.Results:The differences of the serum levels of estradiol and testosterone in male between two groups were statistically significant(PPP>0.05).The serum level of estradiol in male patient with mild illness was moderate,and high in severe patients(PP>0.05).The serum levels of estradiol and testosterone in male patients with small amount of bleeding were higher than those in male patients with median amount of bleeding(PP>0.05).The difference of the estradiol serum level in female between two groups was not statistically significant(P>0.05),but for testosteron difference,it was statistically significant(PPPPPP>0.05).Compared with the no change and deterioration patients,the serum levels of estradiol and testosterone and the ratio of estradiol and testosterone in patients with obvious improvement were significantly increased,decreased and incresed,respectively(PPConclusions:The serum level of estradiol and ratio of estradiol and testosterone in patients with hypertensive intracerebral hemorrhage are helpful for judging the disease prognosis.
Objective:To investigate the serum levels of estradiol and testosterone in patients with hypertensive intracerebral hemorrhage and their relations with illness severity and prognosis.Methods:The serum levels of estradiol and testosterone in 168 patients with hypertensive intracerebral hemorrhage(cerebral hemorrhage group) and 60 healthy people(control group) were determined.All patients were scored by U.S.National Institutes of Health Stroke Scale,and compared in two groups.Results:The differences of the serum levels of estradiol and testosterone in male between two groups were statistically significant(PPP>0.05).The serum level of estradiol in male patient with mild illness was moderate,and high in severe patients(PP>0.05).The serum levels of estradiol and testosterone in male patients with small amount of bleeding were higher than those in male patients with median amount of bleeding(PP>0.05).The difference of the estradiol serum level in female between two groups was not statistically significant(P>0.05),but for testosteron difference,it was statistically significant(PPPPPP>0.05).Compared with the no change and deterioration patients,the serum levels of estradiol and testosterone and the ratio of estradiol and testosterone in patients with obvious improvement were significantly increased,decreased and incresed,respectively(PPConclusions:The serum level of estradiol and ratio of estradiol and testosterone in patients with hypertensive intracerebral hemorrhage are helpful for judging the disease prognosis.
2014, 38(6): 749-751.
Abstract:
Objective:To evaluate the effects of psychological intervention on the depressive emotion of community patients with malignant tumors.Methods:Eighty-one depression patients with malignant tumor from Yingbo community were randomly divided into control group(40 cases) and intervention group(41 cases).The control group were treated with the routine rehabilitation,and the intervention group were treated with psychological intervention based on routine rehabilitation,respectively.The Hamilton's scales in two groups were compared before treatment and 3 months after treatment.Results:The Hamilton's scale in the intervention group was significantly better than that in the control group after treatment(PConclusions:Psychological intervention can improve the depressive emotion,life confidence and quality of community patients with malignant tumor.
Objective:To evaluate the effects of psychological intervention on the depressive emotion of community patients with malignant tumors.Methods:Eighty-one depression patients with malignant tumor from Yingbo community were randomly divided into control group(40 cases) and intervention group(41 cases).The control group were treated with the routine rehabilitation,and the intervention group were treated with psychological intervention based on routine rehabilitation,respectively.The Hamilton's scales in two groups were compared before treatment and 3 months after treatment.Results:The Hamilton's scale in the intervention group was significantly better than that in the control group after treatment(PConclusions:Psychological intervention can improve the depressive emotion,life confidence and quality of community patients with malignant tumor.
2014, 38(6): 752-753,756.
Abstract:
Objective:To observe the curative effects of Albuterol combined with budesonide in the treatment of asthma acute attack in children and its effects on pulmonary function.Methods:One hundred and twenty-six children were divided into observation group and control group.The control group were treated with anti-infection,and regular inhaling corticosteroids and oxygen,the observation group were treated with Salbutamol and Budesonide on the basis of the control group treatment.Results:The total effective rates of the observation group and control group were 95.24% and 73.02%，respectively.The therapeutic effects of observation group were better than that of control group(PPPPPConclusions:The treatment of Salbutamol combined with Budesonide can improve the clinical efficacy of children with asthma acute attack,which is the ideal,safe and effective therapy method.
Objective:To observe the curative effects of Albuterol combined with budesonide in the treatment of asthma acute attack in children and its effects on pulmonary function.Methods:One hundred and twenty-six children were divided into observation group and control group.The control group were treated with anti-infection,and regular inhaling corticosteroids and oxygen,the observation group were treated with Salbutamol and Budesonide on the basis of the control group treatment.Results:The total effective rates of the observation group and control group were 95.24% and 73.02%，respectively.The therapeutic effects of observation group were better than that of control group(PPPPPConclusions:The treatment of Salbutamol combined with Budesonide can improve the clinical efficacy of children with asthma acute attack,which is the ideal,safe and effective therapy method.
2014, 38(6): 754-756.
Abstract:
Objective:To preliminarily analyze of the effect of preoperative score and rating on postoperative complications of colon polyps.Methods:According to the number of polyps,size,shape,pathological,total of 213 cases of colon polyps were scored and rated before endoscopic treatment of colon polyps,analyzed the impact of postoperative complications.Results:According to preoperative scores and ratings,choosing different endoscopic treatment,no case of serious postoperative complicationg was occurred in 213 patients with colon polyps.Conclusions:According to preoperative scores and ratings,choosing different endoscopic treatment can reduce postoperative complications of colon polyp.
Objective:To preliminarily analyze of the effect of preoperative score and rating on postoperative complications of colon polyps.Methods:According to the number of polyps,size,shape,pathological,total of 213 cases of colon polyps were scored and rated before endoscopic treatment of colon polyps,analyzed the impact of postoperative complications.Results:According to preoperative scores and ratings,choosing different endoscopic treatment,no case of serious postoperative complicationg was occurred in 213 patients with colon polyps.Conclusions:According to preoperative scores and ratings,choosing different endoscopic treatment can reduce postoperative complications of colon polyp.
2014, 38(6): 757-759.
Abstract:
Objective:To explore the clinical effects of manual reduction and plasticity splint fixation combined with traditional Chinese medicine packet in the treatment of ankle fracture.Methods:Fifty-four patients with ankle fracture were treated with manual reduction and plasticity splint fixation,traditional Chinese medicine packet and early functional exercise..Results:All patients were followed up for 10 to 20 months.Excellent in 36 cases(66.7%),good in 17 cases(31.5%) and poor in 1 case(1.9%) were found according to the prognosis assessment of Olerud-Molander ankle fracture scoring system.Conclusions:The clinical effects of manual reduction and plasticity splint fixation combined with traditional Chinese medicine packet in the treatment of ankle fracture are good.The treatment is optimal choice for the patients with fear of surgery,financial difficulty and other reasons.
Objective:To explore the clinical effects of manual reduction and plasticity splint fixation combined with traditional Chinese medicine packet in the treatment of ankle fracture.Methods:Fifty-four patients with ankle fracture were treated with manual reduction and plasticity splint fixation,traditional Chinese medicine packet and early functional exercise..Results:All patients were followed up for 10 to 20 months.Excellent in 36 cases(66.7%),good in 17 cases(31.5%) and poor in 1 case(1.9%) were found according to the prognosis assessment of Olerud-Molander ankle fracture scoring system.Conclusions:The clinical effects of manual reduction and plasticity splint fixation combined with traditional Chinese medicine packet in the treatment of ankle fracture are good.The treatment is optimal choice for the patients with fear of surgery,financial difficulty and other reasons.
2014, 38(6): 760-762.
Abstract:
Objective:To investigate the clinical effects of the one-stage resection and anastomosis in the treatment of left colon carcinoma complicated with acute obstruction.Methods:The left colon carcinoma complicated with acute obstruction patients treated with one-staged resection and anastomosis and traditional two-stage anastomosis were divided into the observation group(27 cases) and control group(19 cases),respectively.The postoperative exhaust time,intraoperative blood loss,hospital stay,complications and 3-year survival rate between two groups were compared.Results:The differences of the postoperative exhaust time,intraoperative blood loss,hospital stay and complications between two groups were not statistically signifficant(P>0.05).The 3-year survival rates of observation group and control group were 92.5% and 63.2%,respectively,the difference of which was statistically significant(PConclusions:The one-stage resection and anastomosis in the treatment of left colon carcinoma complicated with acute obstruction is safe and reliable,which can improve the long-term survival rate,and is worth clinical promotion.
Objective:To investigate the clinical effects of the one-stage resection and anastomosis in the treatment of left colon carcinoma complicated with acute obstruction.Methods:The left colon carcinoma complicated with acute obstruction patients treated with one-staged resection and anastomosis and traditional two-stage anastomosis were divided into the observation group(27 cases) and control group(19 cases),respectively.The postoperative exhaust time,intraoperative blood loss,hospital stay,complications and 3-year survival rate between two groups were compared.Results:The differences of the postoperative exhaust time,intraoperative blood loss,hospital stay and complications between two groups were not statistically signifficant(P>0.05).The 3-year survival rates of observation group and control group were 92.5% and 63.2%,respectively,the difference of which was statistically significant(PConclusions:The one-stage resection and anastomosis in the treatment of left colon carcinoma complicated with acute obstruction is safe and reliable,which can improve the long-term survival rate,and is worth clinical promotion.
2014, 38(6): 762-764.
Abstract:
Objective:To compare the the controlling results of early morning hypertension by amlodipine besylate and valsartan.Methods:200 patients with essential hypertension in self-management hypertensive patients in community were selected.Then 88 morning hypertensive patients were screened out.And antihypertensive medications originally were abandoned for 14 days.The patients were divided into two groups randomly.And 41 patients were asked to take amlodipine besylate as the experimental group,while the other 47 patients taking valsartan as controlling group.The morning blood pressure for four weeks(home self-test blood pressure) were observed to understand the controlling results of early morning hypertension.Results:Both drugs can significantly lower blood pressure(PPConclusions:Amlodipine besylate and valsartan are both relatively safe and effective long-acting antihypertensive drugs.Amlodipine besylate is better than valsartan on the controlling of morning systolic blood pressure.There is no difference on morning diastolic blood pressure.
Objective:To compare the the controlling results of early morning hypertension by amlodipine besylate and valsartan.Methods:200 patients with essential hypertension in self-management hypertensive patients in community were selected.Then 88 morning hypertensive patients were screened out.And antihypertensive medications originally were abandoned for 14 days.The patients were divided into two groups randomly.And 41 patients were asked to take amlodipine besylate as the experimental group,while the other 47 patients taking valsartan as controlling group.The morning blood pressure for four weeks(home self-test blood pressure) were observed to understand the controlling results of early morning hypertension.Results:Both drugs can significantly lower blood pressure(PPConclusions:Amlodipine besylate and valsartan are both relatively safe and effective long-acting antihypertensive drugs.Amlodipine besylate is better than valsartan on the controlling of morning systolic blood pressure.There is no difference on morning diastolic blood pressure.
2014, 38(6): 765-767.
Abstract:
Objective:To analyze the clinical features,diagnosis and treatment of throat syphilis with pharyngeal pain,pharyngeal foreign body sensation and hoarseness as the first symptoms for reducing its misdiagnosis and mistreatment.Methods:The clinical data of 5 patients with throat syphilis were retrospectively analyzed,and the related literatures were reviewed.Results:The white lesions in pharyngeal were or laryngeal mucosa of five patients were found,which was positive by the toluidine red unheated serum test and treponema pallidum particle agglutination test.The symptoms were disappeared and the titer of toluidine red unheated serum test were decreased after treatment.All patients were followed up for 0.5 to 2 years,and only one case recurred.Conclusions:The definite diagnosis is the key of the treatment of throat syphilis,which bases primarily on the patient's exposure history,typical clinical manifestations and laboratory test.When the exposure history is not clear or definite diagnosis is difficult,the corresponding pathogenic and pathological examination is important.
Objective:To analyze the clinical features,diagnosis and treatment of throat syphilis with pharyngeal pain,pharyngeal foreign body sensation and hoarseness as the first symptoms for reducing its misdiagnosis and mistreatment.Methods:The clinical data of 5 patients with throat syphilis were retrospectively analyzed,and the related literatures were reviewed.Results:The white lesions in pharyngeal were or laryngeal mucosa of five patients were found,which was positive by the toluidine red unheated serum test and treponema pallidum particle agglutination test.The symptoms were disappeared and the titer of toluidine red unheated serum test were decreased after treatment.All patients were followed up for 0.5 to 2 years,and only one case recurred.Conclusions:The definite diagnosis is the key of the treatment of throat syphilis,which bases primarily on the patient's exposure history,typical clinical manifestations and laboratory test.When the exposure history is not clear or definite diagnosis is difficult,the corresponding pathogenic and pathological examination is important.
2014, 38(6): 768-770.
Abstract:
Objective:To study the diagnosis,treatment and prognosis of peripheral artery injury.Methods:The clinical data of 32 cases with peripheral artery injury(including blunt injury in 22 cases,penetrating injury in 7 cases and iatrogenic injury in 3 cases) were retrospectively analyzed.Two,5,1,22 and 2 cases were treated with artery wall repairing,simple ligation,end-to-end anastomosis,autologous vein graft and covered stent inplant,respectively.Results:Twenty-eight cases cured,4 cases were amputated and no case died.The cure rate,amputation rate and mortality were 87.50%,12.50% and 0.00%,respectively.Conclusions:The early diagnosing,effective controlling bleeding,anti-shock treating,correct operating,revascularizating,early cutting fascia and appropriate managing the compound wounds can decrease the mortality and morbidity.
Objective:To study the diagnosis,treatment and prognosis of peripheral artery injury.Methods:The clinical data of 32 cases with peripheral artery injury(including blunt injury in 22 cases,penetrating injury in 7 cases and iatrogenic injury in 3 cases) were retrospectively analyzed.Two,5,1,22 and 2 cases were treated with artery wall repairing,simple ligation,end-to-end anastomosis,autologous vein graft and covered stent inplant,respectively.Results:Twenty-eight cases cured,4 cases were amputated and no case died.The cure rate,amputation rate and mortality were 87.50%,12.50% and 0.00%,respectively.Conclusions:The early diagnosing,effective controlling bleeding,anti-shock treating,correct operating,revascularizating,early cutting fascia and appropriate managing the compound wounds can decrease the mortality and morbidity.
2014, 38(6): 786-788.
Abstract:
Objective:To investigate the current status and management of drinking water among the primary and middle schools in Bozhou city,and to provide scientific basis for the guidance.Methods:Stratified sampling was conducted in 150 primary and middle schools,and 150 samples of drinking water were detected.Results:The overall qualified rate of the drinking water was 64.00%.Ninety-one schools provided central water supply,14 schools had secondary water supply and 45 schools had no drinking water supply.The drinking water quality was evaluated as good in 34 schools,fair in 78 schools and poor in 38.The main unqualified tested items focused on ammonia nitrogen,fluoride,total bacterial count and total coliform group.The water quality in schools with high management level was superior to that of schools with ordinary or poor management levels(PPConclusions:There exists security danger in drinking water among the primary and middle schools in Bozhou city.It is suggested that drinking water security system should be established,health education strengthened and health supervision and guidance enforced.
Objective:To investigate the current status and management of drinking water among the primary and middle schools in Bozhou city,and to provide scientific basis for the guidance.Methods:Stratified sampling was conducted in 150 primary and middle schools,and 150 samples of drinking water were detected.Results:The overall qualified rate of the drinking water was 64.00%.Ninety-one schools provided central water supply,14 schools had secondary water supply and 45 schools had no drinking water supply.The drinking water quality was evaluated as good in 34 schools,fair in 78 schools and poor in 38.The main unqualified tested items focused on ammonia nitrogen,fluoride,total bacterial count and total coliform group.The water quality in schools with high management level was superior to that of schools with ordinary or poor management levels(PPConclusions:There exists security danger in drinking water among the primary and middle schools in Bozhou city.It is suggested that drinking water security system should be established,health education strengthened and health supervision and guidance enforced.
2014, 38(6): 790-792.
Abstract:
Objective:To investigate the value of echocardiography in diagnosis of the rupture of aortic simusal aneurysm and its complications.Methods:Eighteen patients were detected to have the rupture of aortic simusal aneurysm by echocardiography,and the result was compared with that by pathology examination.Results:Seventeen cases were confirmed to have the rupture of aortic sinus aneurysm by echocardiography,and its diagnosis accordance rate with the result by pathology reached 94.44%;12 cases were confirmed to have complications by echocardiography,and the diagnosis accordance rate with the result by pathology was 92.31%.Conclusions:The echocardiography is in high accordance with pathology examination in diagnosis of the rupture of aortic sinus aneurysm and its complications;it may provide a reliable basis for the choice of therapeutic regimens.It is the preferred method of inspection.
Objective:To investigate the value of echocardiography in diagnosis of the rupture of aortic simusal aneurysm and its complications.Methods:Eighteen patients were detected to have the rupture of aortic simusal aneurysm by echocardiography,and the result was compared with that by pathology examination.Results:Seventeen cases were confirmed to have the rupture of aortic sinus aneurysm by echocardiography,and its diagnosis accordance rate with the result by pathology reached 94.44%;12 cases were confirmed to have complications by echocardiography,and the diagnosis accordance rate with the result by pathology was 92.31%.Conclusions:The echocardiography is in high accordance with pathology examination in diagnosis of the rupture of aortic sinus aneurysm and its complications;it may provide a reliable basis for the choice of therapeutic regimens.It is the preferred method of inspection.
2014, 38(6): 793-794,797.
Abstract:
Objective:To study the preoperative application value of magnetic resonance arteriography(MRA) and magnetic resonance venography(MRV) in intracranial tumor.Methods:Twenty patients with intracranial tumor dignosed by pathology were detected by MRI,MRA and/or MRV before operation.The preoperative image data were compared with the intraoperative findings.Results:Among 18 cases examined by MRA,4 cases with blood supply artery abnormal enlargement and 6 cases with intracranial major vascular oppressed,moved and embedded by tumor were found.Among 12 cases examined by MRV,6 cases with venous sinus invaded and 4 cases with venous sinus oppressed and moved by tumor were found.Three cases with draining vein enlargement were found.The intraoperative findings were highly consistent with the preoperative MRA and MRV images.Conclusions:MRA and MRV can clearly display the anatomical relationships between intracranial tumor and major blood vessel,which can provide the precise image information for formulating reasonable operation scheme.
Objective:To study the preoperative application value of magnetic resonance arteriography(MRA) and magnetic resonance venography(MRV) in intracranial tumor.Methods:Twenty patients with intracranial tumor dignosed by pathology were detected by MRI,MRA and/or MRV before operation.The preoperative image data were compared with the intraoperative findings.Results:Among 18 cases examined by MRA,4 cases with blood supply artery abnormal enlargement and 6 cases with intracranial major vascular oppressed,moved and embedded by tumor were found.Among 12 cases examined by MRV,6 cases with venous sinus invaded and 4 cases with venous sinus oppressed and moved by tumor were found.Three cases with draining vein enlargement were found.The intraoperative findings were highly consistent with the preoperative MRA and MRV images.Conclusions:MRA and MRV can clearly display the anatomical relationships between intracranial tumor and major blood vessel,which can provide the precise image information for formulating reasonable operation scheme.
2014, 38(6): 795-797.
Abstract:
Objective:To investigate the level changes of cytokines during the radioactive iodine(131I) treatment of Graves disease and its clinical significance.Methods:The serum levels of cytokines including interleukin-1β(IL-1β),solubility interleukin-2R(sIL-2R),IL-5,IL-6 and tumor necrosis factor-α(TNF-α) in the patients with Graves disease(n=61),the cured patients treated with 131I(n=65) and health control group(n=68) were measured by ELISA.The relationships between cytokines and thyroid hormone were analyzed.Results:The levels of IL-1β,sIL-2R,IL-5,IL-6 and TNF-α in patients with Graves disease and the cured patients were significantly higher than those in heath control group(PPP131I and health control group was not statistically significant(P>0.05).The levels of serum IL-1β and free thyroxine,sIL-2R and serum thyrotropin receptor antibody(TRAb),and TNF-α and TRAb were positive correlations(PConclusions:The serum cytokines levels are closely related to the function of thyroid,which can be used as an evaluation index of radioactive iodine treatment effects for patients with hyperthyroidism.
Objective:To investigate the level changes of cytokines during the radioactive iodine(131I) treatment of Graves disease and its clinical significance.Methods:The serum levels of cytokines including interleukin-1β(IL-1β),solubility interleukin-2R(sIL-2R),IL-5,IL-6 and tumor necrosis factor-α(TNF-α) in the patients with Graves disease(n=61),the cured patients treated with 131I(n=65) and health control group(n=68) were measured by ELISA.The relationships between cytokines and thyroid hormone were analyzed.Results:The levels of IL-1β,sIL-2R,IL-5,IL-6 and TNF-α in patients with Graves disease and the cured patients were significantly higher than those in heath control group(PPP131I and health control group was not statistically significant(P>0.05).The levels of serum IL-1β and free thyroxine,sIL-2R and serum thyrotropin receptor antibody(TRAb),and TNF-α and TRAb were positive correlations(PConclusions:The serum cytokines levels are closely related to the function of thyroid,which can be used as an evaluation index of radioactive iodine treatment effects for patients with hyperthyroidism.
2014, 38(6): 798-801.
Abstract:
Objective:To explore the correlation between human sperm DNA integrity and related semen parameters in male infertility.Methods:Semen samples of male infertients(100 cases) were collected.Sperm chromatin dispersion experiment was used to detect the sperm DNA integrity,mixed agglutination reaction test was used to detect the sperm antibody and computer-aided semen analysis system was performed to detect the seminal parameters.Results:Age was independent of sperm fragmentation rate,semen parameters and anti-sperm antibody(P>0.05).Sperm DNA fragmentation rate was negatively correlated with sperm density and sperm motility(PP>0.05).Anti-sperm antibody was weakly correlated with wobble and straightness(PConclusions:Sperm DNA integrity and anti-sperm antibody as the supplementary index of semen analysis have important clinical significance.
Objective:To explore the correlation between human sperm DNA integrity and related semen parameters in male infertility.Methods:Semen samples of male infertients(100 cases) were collected.Sperm chromatin dispersion experiment was used to detect the sperm DNA integrity,mixed agglutination reaction test was used to detect the sperm antibody and computer-aided semen analysis system was performed to detect the seminal parameters.Results:Age was independent of sperm fragmentation rate,semen parameters and anti-sperm antibody(P>0.05).Sperm DNA fragmentation rate was negatively correlated with sperm density and sperm motility(PP>0.05).Anti-sperm antibody was weakly correlated with wobble and straightness(PConclusions:Sperm DNA integrity and anti-sperm antibody as the supplementary index of semen analysis have important clinical significance.
2014, 38(6): 802-803.
Abstract:
Objective:To investigate the infection status of infectious diseases in patients before blood transfusion so as to prevent hospital infection,infection of the occupational health care workers and medical disputes due to blood transfusion.Methods:Six hundred and twenty blood recipients were measured of the contagious serum markers including HBV markers(HBsAg,anti-HBs,HBeAg,anti-HBe,anti-HBe),anti-HCV,anti-HIV and anti-TP with ELISA before blood transfusion.Results:The positive rates of HBsAg,anti-HCV,anti-HIV and anti-TP in the 620 patients before blood transfusion were 8.71%,1.77%,0.16% and 1.94%,respectively.Conclusions:It is of great clinical significance to test the contagious serum markers of the blood receivers before blood transfusion,which can reveal the condition of the blood recipients,ensure the safety of blood transfusion,decrease medical dissatisfaction and prevent hospital infection.
Objective:To investigate the infection status of infectious diseases in patients before blood transfusion so as to prevent hospital infection,infection of the occupational health care workers and medical disputes due to blood transfusion.Methods:Six hundred and twenty blood recipients were measured of the contagious serum markers including HBV markers(HBsAg,anti-HBs,HBeAg,anti-HBe,anti-HBe),anti-HCV,anti-HIV and anti-TP with ELISA before blood transfusion.Results:The positive rates of HBsAg,anti-HCV,anti-HIV and anti-TP in the 620 patients before blood transfusion were 8.71%,1.77%,0.16% and 1.94%,respectively.Conclusions:It is of great clinical significance to test the contagious serum markers of the blood receivers before blood transfusion,which can reveal the condition of the blood recipients,ensure the safety of blood transfusion,decrease medical dissatisfaction and prevent hospital infection.
2014, 38(6): 804-806.
Abstract:
Objective:To investigate the clinical value of fluorescent quatititive polymerase chain reaction(FQ-PCR) in the detection of hepatitis B Virus(HBV)-DNA.Methods:The positive rate and copies of HBV-DNA in 265 HBV patients and 150 healthy people were detected using FQ-PCR,which were compared with the serological markers of hepatitis B detected by enzyme linked immunosorbent assay.Results:The HBV-DNA was detected in all cases with different serological markers,the positive rate and copies of HBV-DNA in HBsAg,HBeAg and HBcAb(+) group and HBsAg and HBeAg(+) group were higher than those in other groups(PP>0.05),the copies of HBV in acute hepatitis patients were significantly higher than that in other groups(PConclusions:The HBV-DNA detected by FQ-PCR can directly reflect the HBV infection,judge the ability of replication and infection,guide the clinical medication and observe curative effects.
Objective:To investigate the clinical value of fluorescent quatititive polymerase chain reaction(FQ-PCR) in the detection of hepatitis B Virus(HBV)-DNA.Methods:The positive rate and copies of HBV-DNA in 265 HBV patients and 150 healthy people were detected using FQ-PCR,which were compared with the serological markers of hepatitis B detected by enzyme linked immunosorbent assay.Results:The HBV-DNA was detected in all cases with different serological markers,the positive rate and copies of HBV-DNA in HBsAg,HBeAg and HBcAb(+) group and HBsAg and HBeAg(+) group were higher than those in other groups(PP>0.05),the copies of HBV in acute hepatitis patients were significantly higher than that in other groups(PConclusions:The HBV-DNA detected by FQ-PCR can directly reflect the HBV infection,judge the ability of replication and infection,guide the clinical medication and observe curative effects.
2014, 38(6): 809-811.
Abstract:
Objective:To investigate the effects of Peer-Assisted Learning in the safety education course of medical students.Methods:Two hundred and six students from a clinical medical department of 2013 class A(the observation group) were divided into 20 Peer-assisted groups,who were taught using Peer-Assisted Learning method.Two hundred and one students from class B(the control group) were taught using the traditional lecturing method.The summative evaluation,teaching experience and satisfaction degree between two groups were compared.Results:The final summary evaluation scores of the observation group were significantly higher than that in control group(PPConclusions:Peer-Assisted Learning can improve the medical students interest of learning safety education course and abilities to solve practical security problems,which can further enhance the teaching effects.
Objective:To investigate the effects of Peer-Assisted Learning in the safety education course of medical students.Methods:Two hundred and six students from a clinical medical department of 2013 class A(the observation group) were divided into 20 Peer-assisted groups,who were taught using Peer-Assisted Learning method.Two hundred and one students from class B(the control group) were taught using the traditional lecturing method.The summative evaluation,teaching experience and satisfaction degree between two groups were compared.Results:The final summary evaluation scores of the observation group were significantly higher than that in control group(PPConclusions:Peer-Assisted Learning can improve the medical students interest of learning safety education course and abilities to solve practical security problems,which can further enhance the teaching effects.
2014, 38(6): 812-815.
Abstract:
Objective:To find out ways of improving the nursing students' professional ability and the pass rate in Chinese Licensure Examination for Nurse(CLEN) in higher vocational colleges.Methods:On the basis of studying 2011 CLEN syllabus,we recompiled the textbook,redrew up the teaching plan and reformed the teaching and examination methods.Results:The nursing students' professional ability and CLEN pass rate improved significantly.In 2011,709 of the 723 graduates who had participated in CLEN passed the examination,with a pass rate of 98.06%;and in 2012,777 of the 780 graduates who had participated in the CLEN passed,with a pass rate of 99.62%.Conclusions:The teaching reform based on CLEN syllabus can improve the students' professional ability and their pass rate in CLEN.
Objective:To find out ways of improving the nursing students' professional ability and the pass rate in Chinese Licensure Examination for Nurse(CLEN) in higher vocational colleges.Methods:On the basis of studying 2011 CLEN syllabus,we recompiled the textbook,redrew up the teaching plan and reformed the teaching and examination methods.Results:The nursing students' professional ability and CLEN pass rate improved significantly.In 2011,709 of the 723 graduates who had participated in CLEN passed the examination,with a pass rate of 98.06%;and in 2012,777 of the 780 graduates who had participated in the CLEN passed,with a pass rate of 99.62%.Conclusions:The teaching reform based on CLEN syllabus can improve the students' professional ability and their pass rate in CLEN.
2014, 38(6): 816-818.
Abstract:
Objective:To assess the value of comparative imageology in nuclear medicine teaching.Methods:Students of grade 2010 from the department of clinical medicine in Bengbu Medical College were selected as the study objects,and the class was divided into traditional teaching group and comparative imageological group.The teaching effect was evaluated by a questionnaire survey and the examination performance.Results:The teaching effect of the comparative imageological group was better than that of the traditional teaching group(P＜0.01).Conclusions:The introduction of comparative imageology in nuclear medicine teaching may stimulate the students' learning interest,improve their learning efficiency and help them make rational selection in imaging diagnosis methods,which would be of great value for their future clinical practice and work.
Objective:To assess the value of comparative imageology in nuclear medicine teaching.Methods:Students of grade 2010 from the department of clinical medicine in Bengbu Medical College were selected as the study objects,and the class was divided into traditional teaching group and comparative imageological group.The teaching effect was evaluated by a questionnaire survey and the examination performance.Results:The teaching effect of the comparative imageological group was better than that of the traditional teaching group(P＜0.01).Conclusions:The introduction of comparative imageology in nuclear medicine teaching may stimulate the students' learning interest,improve their learning efficiency and help them make rational selection in imaging diagnosis methods,which would be of great value for their future clinical practice and work.
2014, 38(6): 823-825,828.
Abstract:
Objective:To understand the current situation and its influencing factors of nursing humanistic caring capability in some comprehensive hospitals of a city.Methods:Two hundred and sixty-eight nurses in three 3A grade hospitals in a city were investigated and analyzed via nursing humanistic caring capability scale.Results:The total score of nursing humanistic caring capability was 135.79±12.15,there were statistical differences in nurses with different ages,professional titles,educational backgrounds and marriage situations(P＜0.01).Among them,the score of inculcation belief and expectation was 25.65±1.63,the score of health education was 21.48±1.71,the score of humanity and altruism value was 19.36±3.48,the score of scientific solution to health problem was 13.58±2.53,the score of assistance in meeting basic requirement was 10.62±1.91,the score of providing good environment was 13.82±3.62,the score of promoting emotion communication was 16.79±4.73 and the score of helping to solve the difficulties was 14.75±3.28.Conclusions:Educational background,professional title,age and marriage situation were the influencing factors of nursing humanistic caring capability,nursing administrators should implement hierarchical continuing education and training to carry out high quality nursing services according to all kinds of influencing factors.
Objective:To understand the current situation and its influencing factors of nursing humanistic caring capability in some comprehensive hospitals of a city.Methods:Two hundred and sixty-eight nurses in three 3A grade hospitals in a city were investigated and analyzed via nursing humanistic caring capability scale.Results:The total score of nursing humanistic caring capability was 135.79±12.15,there were statistical differences in nurses with different ages,professional titles,educational backgrounds and marriage situations(P＜0.01).Among them,the score of inculcation belief and expectation was 25.65±1.63,the score of health education was 21.48±1.71,the score of humanity and altruism value was 19.36±3.48,the score of scientific solution to health problem was 13.58±2.53,the score of assistance in meeting basic requirement was 10.62±1.91,the score of providing good environment was 13.82±3.62,the score of promoting emotion communication was 16.79±4.73 and the score of helping to solve the difficulties was 14.75±3.28.Conclusions:Educational background,professional title,age and marriage situation were the influencing factors of nursing humanistic caring capability,nursing administrators should implement hierarchical continuing education and training to carry out high quality nursing services according to all kinds of influencing factors.
2014, 38(6): 826-828.
Abstract:
Objective:To investigate the effects of humanized nursing service mode in improving patient's satisfaction and nursing quality.Methods:The humanized nursing service mode and routine nursing service mode were implemented from Jan.2012 to Jan.2013(the experimental stage) and from Jan.2011 to Dec.2011(the control stage) in phthalmic outpatient nursing management,respectively.The nursing satisfaction of patient,work quality of nursing staff,and the percentage of nursing dispute of ophthalmology dispute between two stages were compared.Results:Compared with the control stage,the scores of nursing technology,health guidance,psychological care,hospitality,nursing performance and service attitude of nurse and patient's satisfaction significantly increased in experimental stage(PPP=0.017).Conclusions:The humanized nursing service mode in ophthalmic outpatient nursing management can improve patient's satisfaction and work quality of nursing staff,which is conducive to reducing the occurrence of the dispute of nurse-patient,and establishing their good relations.
Objective:To investigate the effects of humanized nursing service mode in improving patient's satisfaction and nursing quality.Methods:The humanized nursing service mode and routine nursing service mode were implemented from Jan.2012 to Jan.2013(the experimental stage) and from Jan.2011 to Dec.2011(the control stage) in phthalmic outpatient nursing management,respectively.The nursing satisfaction of patient,work quality of nursing staff,and the percentage of nursing dispute of ophthalmology dispute between two stages were compared.Results:Compared with the control stage,the scores of nursing technology,health guidance,psychological care,hospitality,nursing performance and service attitude of nurse and patient's satisfaction significantly increased in experimental stage(PPP=0.017).Conclusions:The humanized nursing service mode in ophthalmic outpatient nursing management can improve patient's satisfaction and work quality of nursing staff,which is conducive to reducing the occurrence of the dispute of nurse-patient,and establishing their good relations.
Clinical observation of a new type of dressing for management of venous ulcer in the lower extremity
2014, 38(6): 829-830.
Abstract:
Objective:To investigate the effect of a new type of dressing in the management of venous ulcer wound in the lower extremity.Methods:Seventy cases of venous ulcer in the lower extremity were randomly divided into 2 groups(n=35 in each).The control group were applied the traditional dressing,while the treatment group adopted the new type of dressing.The clinical efficacy was observed in the 2 groups.Results:The total effective rate of the treatment group was 100.00％,which was significantly higher than that of the control group(71.43%)(PPConclusions:The new type of dressing is superior to the traditional one in management of the venous ulceration in the lower limb.It is worth popularizing.
Objective:To investigate the effect of a new type of dressing in the management of venous ulcer wound in the lower extremity.Methods:Seventy cases of venous ulcer in the lower extremity were randomly divided into 2 groups(n=35 in each).The control group were applied the traditional dressing,while the treatment group adopted the new type of dressing.The clinical efficacy was observed in the 2 groups.Results:The total effective rate of the treatment group was 100.00％,which was significantly higher than that of the control group(71.43%)(PPConclusions:The new type of dressing is superior to the traditional one in management of the venous ulceration in the lower limb.It is worth popularizing.
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