银杏叶提取物(EGb761)对鼠脑缺血半暗带神经细胞凋亡及超微结构的影响

李建军; 张晓雷; 王小姗; 孙琦; 张玲如

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

银杏叶提取物(EGb761)对鼠脑缺血半暗带神经细胞凋亡及超微结构的影响

李建军 , 张晓雷 , 王小姗 , 孙琦 , 张玲如

文章导航 > 蚌埠医学院学报 > 2008, 33 > 4: (400-402)

引用本文:

Citation:

银杏叶提取物(EGb761)对鼠脑缺血半暗带神经细胞凋亡及超微结构的影响

1. 安徽省天长市人民医院神经科, 239300;
2. 南京医科大学附属南京脑科医院神经科, 江苏南京 210029

作者简介: 李建军(1971-),男,主治医师.

中图分类号: R743.31

Influence of Ginkgo Biloba Extract on neural cell apoptosis and ultrastructure in ischemic penumbra after cerebral ischemia/reperfusion in rats

1. Department of Neurology, Tianchang People's Hospital, Tianchang Anhui 239300;
2. Department of Neurology, Nanjing Brain Hospital, Nanjing Medical University, Nanjing Jiangsu 210029, China
CLC number: R743.31

摘要: 目的:探讨银杏叶提取物(EGb761)对鼠脑缺血半暗带神经细胞凋亡及细胞超微结构的影响。方法:雄性Wistar大鼠30只,随机分为假手术组、对照组(包括缺血90 min和缺血90 min再灌注12 h两个亚组)、治疗组(亦包括缺血90 min和缺血90 min再灌注12 h两个亚组),每组6只。治疗组于缺血前和缺血后即刻经腹腔注射EGb761溶液(每次20 mg/kg),假手术组和对照组均于相同时限腹腔注射等容量的生理盐水。建立大鼠大脑中动脉闭塞(MCAO)/再灌注模型。采用原位末端标记法,观察各组鼠脑缺血半暗带神经细胞凋亡,并结合电镜观察缺血神经细胞超微结构的改变。结果:对照组缺血90 min再灌注12 h缺血半暗带可见大量凋亡细胞,凋亡细胞计数为21.2±3.8,较药物治疗组缺血90 min再灌注12 h缺血的9.1±2.3显著增加(P<0.01)。对照组缺血90 min再灌注12 h鼠脑组织电镜检查可见神经元细胞核染色质边聚,呈团块状,线粒体可见絮状结构改变;相同时限药物治疗组脑组织神经元细胞核染色质分布比较均匀,胞质内线粒体及其它细胞器基本正常。结论:血小板活化因子受体拮抗剂EGb761具有显著的缺血后脑保护作用。
- 脑缺血 /
- 再灌注损伤 /
- 银杏叶提取物 /
- 缺血半暗带 /
- 细胞凋亡 /
- 大鼠,近交系
Abstract: Objective: To investigate the influence of Ginkgo Biloba Extract on neural cell apoptosis and urtra micro-structure of neural cells in cerebral ischemic penumbra after cerebral ischemia/reperfusion in rats.Methods: Thirty male Wister rats were randomly divided into sham-operation group(n=6),control group(n=6) and treatment group(n=6).The treatment group were injected intraperitoneally Ginkgo Biloba Extract 761(20 mg/kg) before and after middle cerebral artery occlusion;the sham-operation group and control group were injected normal saline(20 mg/kg).Animal models were established by middle cerebral artery occlusion.The number of nerual cell apoptosis was measured by thymidine/deoxythymidine mediated dexoy UTP-biotin nick and labeling(TUNEL),and the changes of ultra micro-structure of neural cells were observed by electromicroscope.Results: The number of neural cell apoptosis was 21.2±3.8 in control group after ischemia 90 min/reperfusion 12 h,versus 9.1±2.3 in Ginkgo Biloba Extract-treated group after ischemia 90 min/reperfusion 12 h,the difference was significant(P<0.01).The neuron apoptotic cells were observed by their fragmented nuclei condensed chromatin,fragmented or condensed cytoplasm and floccule of mitochondrion in the control group after ischemia 90 min/reperfusion 12 h;whereas the distribution of chromatin was well-proportioned,and normal mitochondrion and other organelle were observed in the Ginkgo Biloba Extract-treated group.Conclusions: As platelet-activating factor receptor antagonists,Ginkgo Biloba Extract has remarkable neuroprotective effect.
- cerebral ischemia /
- reperfusion injury /
- Ginkgo Biloba Extract /
- ischemic penumbra /
- cells apoptosis /
- rats,inbred strains

[1]	侯晞 , 梁枫 , 李爱剑 , 王灿 , 戴体俊 , 桂常青 . 银杏叶提取物联合依达拉奉对气虚血瘀型脑缺血再灌注损伤大鼠脑组织NO及NOS表达的影响. 蚌埠医学院学报, 2011, 36(7): 673-676.
[2]	董银凤 , 陈前芬 . 大豆异黄酮对大鼠脑缺血再灌后ATP酶及bcl-2和bax的影响. 蚌埠医学院学报, 2012, 36(1): 8-11.
[3]	朱磊 , 傅鸣郁 , 王明光 , 田静 , 何秀华 , 安新江 . 银杏叶提取物对急性心肌梗死大鼠心肌细胞的保护作用. 蚌埠医学院学报, 2011, 36(4): 335-338.
[4]	董银凤 , 陈前芬 . 硫酸镁对小鼠脑缺血再灌注损伤后一氧化氮及ATP酶的影响. 蚌埠医学院学报, 2010, 35(4): 330-333.
[5]	杨锡兰 , 王盼 , 董银凤 , 赵士弟 , 陈前芬 . 大豆异黄酮和法舒地尔联合应用对大鼠脑缺血再灌注损伤的保护作用. 蚌埠医学院学报, 2016, 41(6): 712-716. doi: 10.13898/j.cnki.issn.1000-2200.2016.06.003
[6]	余晴晴 , 柏建峰 , 王江军 . 黄芪注射液对脑缺血再灌注损伤大鼠脑内血管新生及HIF-1α/VEGF信号转导通路的影响. 蚌埠医学院学报, 2017, 42(10): 1309-1313. doi: 10.13898/j.cnki.issn.1000-2200.2017.10.005
[7]	朱旭友 , 吕鸿燕 , 赵士弟 , 陈前芬 . 硫酸镁对小鼠全脑缺血再灌注损伤模型NO、NOS的影响. 蚌埠医学院学报, 2009, 34(11): 956-958.
[8]	吕鸿燕 , 朱旭友 , 陈前芬 . 硫酸镁预处理对小鼠全脑缺血再灌注损伤后一氧化氮及一氧化氮合酶的影响. 蚌埠医学院学报, 2009, 34(9): 762-764.
[9]	徐丙发 , 吴华璞 , 祝晓光 . 姜黄素对大鼠缺血再灌注损伤的保护作用. 蚌埠医学院学报, 2007, 32(6): 637-639,765.
[10]	王栓虎 , 姜从桥 . 硫普罗宁和脾切除对大鼠肝脏缺血再灌注损伤的保护作用. 蚌埠医学院学报, 2009, 34(11): 962-965.
[11]	葛嫣然 , 邵宏超 , 王福海 . 雌激素对兔视网膜缺血再灌注损伤中视网膜神经节细胞凋亡及bcl-2表达的影响. 蚌埠医学院学报, 2015, 40(1): 11-13. doi: 10.13898/j.cnki.issn.1000-2200.2015.01.004
[12]	张远海 . 桃红四物汤对脑缺血再灌注损伤大鼠脑内血管新生及PI3K/AKT信号转导通路的影响. 蚌埠医学院学报, 2017, 42(1): 36-40,56. doi: 10.13898/j.cnki.issn.1000-2200.2017.01.010
[13]	黄丽 , 王春 , 赵士弟 . 针刺预处理对大鼠小脑浦肯野细胞缺血性损伤的影响. 蚌埠医学院学报, 2016, 41(1): 7-9,15. doi: 10.13898/j.cnki.issn.1000-2200.2016.01.002
[14]	朱莉文 , 孙彩敬 . 银杏叶提取物对地卓西平致精神分裂症模型大鼠的改善作用及其机制研究. 蚌埠医学院学报, 2022, 47(12): 1619-1622. doi: 10.13898/j.cnki.issn.1000-2200.2022.12.001
[15]	于影 , 胡杰 , 高琴 , 汪思应 . 大鼠肾下腹主动脉缺血再灌注对心肌肌浆网钙泵活性及其基因表达的影响. 蚌埠医学院学报, 2010, 35(2): 112-114.
[16]	郑璇 , 马宜传 , 沈俊杰 , 徐加利 , 陈偏偏 , 谢宗玉 . 急性缺血性脑梗死头颅CT灌注参数与侧支循环分级的相关性分析. 蚌埠医学院学报, 2020, 45(12): 1692-1696. doi: 10.13898/j.cnki.issn.1000-2200.2020.12.026
[17]	李晓红 , 金钟 , 薛梅 , 王南海 . 硬膜外阻滞复合异氟烷吸入对肝脏缺血再灌注损伤的影响. 蚌埠医学院学报, 2004, 29(4): 309-312.
[18]	凌云志 , 禹莉 , 梁启胜 , 李晓红 , 王南海 . 七氟醚对兔肝脏缺血再灌注损伤的影响. 蚌埠医学院学报, 2012, 36(8): 884-887.
[19]	祝晓光 , 韦颖梅 , 吴华璞 . 脑复灵对大鼠局灶性脑缺血的保护作用. 蚌埠医学院学报, 2004, 29(4): 291-293.
[20]	邵宏超 , 葛嫣然 , 刘岩 , 苏杰 . Fas与FasL在兔视网膜缺血再灌注损伤中的表达及rh-bFGF对其表达的影响. 蚌埠医学院学报, 2016, 41(1): 20-23. doi: 10.13898/j.cnki.issn.1000-2200.2016.01.005

点击查看大图

计量

文章访问数: 3664
HTML全文浏览量: 324
PDF下载量: 5
被引次数: 0

银杏叶提取物(EGb761)对鼠脑缺血半暗带神经细胞凋亡及超微结构的影响

作者简介: 李建军(1971-),男,主治医师.

1. 安徽省天长市人民医院神经科, 239300;
2. 南京医科大学附属南京脑科医院神经科, 江苏南京 210029

收稿日期: 2007-11-06

关键词:

摘要: 目的:探讨银杏叶提取物(EGb761)对鼠脑缺血半暗带神经细胞凋亡及细胞超微结构的影响。方法:雄性Wistar大鼠30只,随机分为假手术组、对照组(包括缺血90 min和缺血90 min再灌注12 h两个亚组)、治疗组(亦包括缺血90 min和缺血90 min再灌注12 h两个亚组),每组6只。治疗组于缺血前和缺血后即刻经腹腔注射EGb761溶液(每次20 mg/kg),假手术组和对照组均于相同时限腹腔注射等容量的生理盐水。建立大鼠大脑中动脉闭塞(MCAO)/再灌注模型。采用原位末端标记法,观察各组鼠脑缺血半暗带神经细胞凋亡,并结合电镜观察缺血神经细胞超微结构的改变。结果:对照组缺血90 min再灌注12 h缺血半暗带可见大量凋亡细胞,凋亡细胞计数为21.2±3.8,较药物治疗组缺血90 min再灌注12 h缺血的9.1±2.3显著增加(P<0.01)。对照组缺血90 min再灌注12 h鼠脑组织电镜检查可见神经元细胞核染色质边聚,呈团块状,线粒体可见絮状结构改变;相同时限药物治疗组脑组织神经元细胞核染色质分布比较均匀,胞质内线粒体及其它细胞器基本正常。结论:血小板活化因子受体拮抗剂EGb761具有显著的缺血后脑保护作用。

English Abstract

Influence of Ginkgo Biloba Extract on neural cell apoptosis and ultrastructure in ischemic penumbra after cerebral ischemia/reperfusion in rats

1. Department of Neurology, Tianchang People's Hospital, Tianchang Anhui 239300;
2. Department of Neurology, Nanjing Brain Hospital, Nanjing Medical University, Nanjing Jiangsu 210029, China

Received Date: 2007-11-06

Abstract: Objective: To investigate the influence of Ginkgo Biloba Extract on neural cell apoptosis and urtra micro-structure of neural cells in cerebral ischemic penumbra after cerebral ischemia/reperfusion in rats.Methods: Thirty male Wister rats were randomly divided into sham-operation group(n=6),control group(n=6) and treatment group(n=6).The treatment group were injected intraperitoneally Ginkgo Biloba Extract 761(20 mg/kg) before and after middle cerebral artery occlusion;the sham-operation group and control group were injected normal saline(20 mg/kg).Animal models were established by middle cerebral artery occlusion.The number of nerual cell apoptosis was measured by thymidine/deoxythymidine mediated dexoy UTP-biotin nick and labeling(TUNEL),and the changes of ultra micro-structure of neural cells were observed by electromicroscope.Results: The number of neural cell apoptosis was 21.2±3.8 in control group after ischemia 90 min/reperfusion 12 h,versus 9.1±2.3 in Ginkgo Biloba Extract-treated group after ischemia 90 min/reperfusion 12 h,the difference was significant(P<0.01).The neuron apoptotic cells were observed by their fragmented nuclei condensed chromatin,fragmented or condensed cytoplasm and floccule of mitochondrion in the control group after ischemia 90 min/reperfusion 12 h;whereas the distribution of chromatin was well-proportioned,and normal mitochondrion and other organelle were observed in the Ginkgo Biloba Extract-treated group.Conclusions: As platelet-activating factor receptor antagonists,Ginkgo Biloba Extract has remarkable neuroprotective effect.