3种微柱凝胶抗人球蛋白卡在检测孕妇IgG血型抗体效价中的应用比较

管政; 张军; 陈丽; 郭博

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3种微柱凝胶抗人球蛋白卡在检测孕妇IgG血型抗体效价中的应用比较

管政 , 张军 , 陈丽 , 郭博

文章导航 > 蚌埠医学院学报 > 2011, 36 > 8: (872-874,877)

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3种微柱凝胶抗人球蛋白卡在检测孕妇IgG血型抗体效价中的应用比较

蚌埠医学院第一附属医院输血科, 安徽蚌埠 233004

作者简介: 管政(1971-),男,硕士,主管技师.

中图分类号: R446.61

Application study of three kinds of microcolumn gel Coombs' test cards in detecting titration of IgG antibody against blood group in pregnant women

Department of Transfusion, The First Affiliated Hospital of Bengbu Medical College, Bengbu Anhui 233004, China
CLC number: R446.61

摘要: 目的: 比较BioXun、DiaMed和BioVue 3种抗人球蛋白检测卡在检测孕妇IgG血型抗体效价中的差异,并分别计算它们的医学决定值。方法: 用3种检测卡和试管法平行检测137例与丈夫ABO血型不合的O型孕妇血清IgG抗-A (B)效价水平。结果: BioXun、DiaMed和BioVue卡测得的IgG抗-A (B)的平均效价分别为56.36(30.76)、111.94(59.02)、104.47(58.08),试管法为28.31(16.41),它们与试管法的差异均有统计学意义(P < 0.01),并均呈正相关关系(r=0.950,0.938;r=0.913,0.901;r=0.873,0.883)。DiaMed卡与BioVue卡测得的结果差异无统计学意义(P > 0.05)。BioXun卡与DiaMed卡或与BioVue卡之间差异均有统计学意义(P < 0.01),但其结果与DiaMed卡和BioVue卡的结果均呈高度正相关关系(r=0.960,0.917;r=0.948,0.940)。通过回归方程计算BioXun、DiaMed和BioVue卡检测孕妇IgG抗-A (B)效价时的医学决定值分别为122(122)、267(228)、215(229)。结论: 3种抗人球蛋白检测卡较之试管法具有高灵敏度,都需重新界定医学决定值。国产BioXun卡的灵敏度低于进口DiaMed和BioVue卡,但其试验结果与进口检测卡试验结果呈高度正相关。进口DiaMed和BioVue抗人球蛋白检测卡具有相同程度的灵敏度。
- 微柱凝胶抗人球蛋白试验 /
- 试管法抗人球蛋白试验 /
- IgG抗体效价
Abstract: Objective: To compare the performance of three kinds of microcolumn gel Coombs' test cards (BioXun, DiaMed and BioVue) in detecting titration of IgG antibody against blood group in pregnant women with blood type O and calculate their critical titres respectively. Methods: Parallel tests using three kinds of microcolumn gel Coombs' test cards and tube Coombs' test were carried out for detecting titration of IgG anti-A or IgG anti-B in 137 pregnant women whose blood types were O and incompatible to that of their husbands. Results: The mean titres of IgG anti-A(B) determined by BioXun,DiaMed and BioVue cards were 56.36(30.76),111.94 (59.02) and 104.47(58.08),respectively,and that determined by test tube Coombs' test was 28.31(16.41). The differences between mean titres which obtained using three kinds of Coombs' cards and which by test tube Coombs' test were significant(P < 0.01). The results of three kinds of Combs' tests were positively correlated with the results of tube Coombs' test(r=0.950,0.938; r=0.913, 0.901; r=0.873,0.883). The results between DiaMed cards and BioVue cards did not show any significant difference(P > 0.05). The differences between BioXun cards and DiaMed or BioVue cards were significant(P < 0.01),but the resuts of BioXun cards were highly correlated with each of them(r=0.960, 0.917; r=0.948, 0.940). Based on regression equations,the critical titres for IgG antibody against blood group IgG anti-A or IgG anti-B in pregnant women using BioXun,DiaMed and BioVue cards were 122(122),267(228) and 215(229), respectively. Conclusions: The three kinds of Coombs'cards are all hypersensitive,need to be redefined their critical titres. The sensitivity of domestic BioXun cards is lower than imported DiaMed or BioVue cards,but it has highly positive correlation with imported cards. The sensitivity of DiaMed and BioVue cards are at the same degree.
- microcolumn gel Coombs test /
- test tube Coombs test /
- titration of IgG antibody

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3种微柱凝胶抗人球蛋白卡在检测孕妇IgG血型抗体效价中的应用比较

作者简介: 管政(1971-),男,硕士,主管技师.

蚌埠医学院第一附属医院输血科, 安徽蚌埠 233004

收稿日期: 2011-04-19

关键词:

摘要: 目的: 比较BioXun、DiaMed和BioVue 3种抗人球蛋白检测卡在检测孕妇IgG血型抗体效价中的差异,并分别计算它们的医学决定值。方法: 用3种检测卡和试管法平行检测137例与丈夫ABO血型不合的O型孕妇血清IgG抗-A (B)效价水平。结果: BioXun、DiaMed和BioVue卡测得的IgG抗-A (B)的平均效价分别为56.36(30.76)、111.94(59.02)、104.47(58.08),试管法为28.31(16.41),它们与试管法的差异均有统计学意义(P < 0.01),并均呈正相关关系(r=0.950,0.938;r=0.913,0.901;r=0.873,0.883)。DiaMed卡与BioVue卡测得的结果差异无统计学意义(P > 0.05)。BioXun卡与DiaMed卡或与BioVue卡之间差异均有统计学意义(P < 0.01),但其结果与DiaMed卡和BioVue卡的结果均呈高度正相关关系(r=0.960,0.917;r=0.948,0.940)。通过回归方程计算BioXun、DiaMed和BioVue卡检测孕妇IgG抗-A (B)效价时的医学决定值分别为122(122)、267(228)、215(229)。结论: 3种抗人球蛋白检测卡较之试管法具有高灵敏度,都需重新界定医学决定值。国产BioXun卡的灵敏度低于进口DiaMed和BioVue卡,但其试验结果与进口检测卡试验结果呈高度正相关。进口DiaMed和BioVue抗人球蛋白检测卡具有相同程度的灵敏度。

English Abstract

Application study of three kinds of microcolumn gel Coombs' test cards in detecting titration of IgG antibody against blood group in pregnant women

Department of Transfusion, The First Affiliated Hospital of Bengbu Medical College, Bengbu Anhui 233004, China

Received Date: 2011-04-19

Abstract: Objective: To compare the performance of three kinds of microcolumn gel Coombs' test cards (BioXun, DiaMed and BioVue) in detecting titration of IgG antibody against blood group in pregnant women with blood type O and calculate their critical titres respectively. Methods: Parallel tests using three kinds of microcolumn gel Coombs' test cards and tube Coombs' test were carried out for detecting titration of IgG anti-A or IgG anti-B in 137 pregnant women whose blood types were O and incompatible to that of their husbands. Results: The mean titres of IgG anti-A(B) determined by BioXun,DiaMed and BioVue cards were 56.36(30.76),111.94 (59.02) and 104.47(58.08),respectively,and that determined by test tube Coombs' test was 28.31(16.41). The differences between mean titres which obtained using three kinds of Coombs' cards and which by test tube Coombs' test were significant(P < 0.01). The results of three kinds of Combs' tests were positively correlated with the results of tube Coombs' test(r=0.950,0.938; r=0.913, 0.901; r=0.873,0.883). The results between DiaMed cards and BioVue cards did not show any significant difference(P > 0.05). The differences between BioXun cards and DiaMed or BioVue cards were significant(P < 0.01),but the resuts of BioXun cards were highly correlated with each of them(r=0.960, 0.917; r=0.948, 0.940). Based on regression equations,the critical titres for IgG antibody against blood group IgG anti-A or IgG anti-B in pregnant women using BioXun,DiaMed and BioVue cards were 122(122),267(228) and 215(229), respectively. Conclusions: The three kinds of Coombs'cards are all hypersensitive,need to be redefined their critical titres. The sensitivity of domestic BioXun cards is lower than imported DiaMed or BioVue cards,but it has highly positive correlation with imported cards. The sensitivity of DiaMed and BioVue cards are at the same degree.