胃癌组织中端粒酶活性与基质金属蛋白酶-7表达的检测及其临床意义
The clinical significance of telomerase activity and MMP-7 expression in gastric carcinoma tissues
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摘要: 目的：检测胃癌组织中的端粒酶活性和基质金属蛋白酶-7(MMP-7)的表达，并探讨其临床意义。方法：在49例胃癌组织(转移30例，未转移19例)和20例癌前病变组织中，采用RT-PCR法检测MMP-7 mRNA，TRAP法检测端粒酶活性。结果：49例胃癌组织中，MMP-7 mRNA及端粒酶阳性表达者分别为28例和32例，其中转移组表达分别为21例和20例；20例癌前病变组织中MMP-7、端粒酶表达分别为4例和8例。胃癌组联合检测均阳性表达者19例，均阴性者8例；癌前病变组联合检测均阳性者仅3例，均阴性者11例。而MMP-7 mRNA表达在性别、年龄间差异无统计学意义(P；0.05)；2组在病理分级中，Ⅰ、Ⅱ级的表达明显低于Ⅲ级(P；0.01)；侵及浆膜层者明显高于未侵及者(P；0.01)。端粒酶在性别、年龄和病理分级以及是否侵及浆膜层间差异均无统计学意义(P；0.05)。结论：MMP-7、端粒酶与胃癌的发生、发展可能有关，高表达者胃癌转移的风险可能较高。联合检测较单一指标的检测率高。Abstract: Objective:To evaluate the therapeutic efficacy of thrombus aspiration technique and thrombolysis of intracavitary and percutaneous transluminal angioplasty(PTA) for Budd-Chiari syndrome(BCS) complicated with inferior vena cava(IVC)thrombus. Methods:The clinical data of 11 cases of BCS complicated with IVC thrombus were collected.Venipuncture through femoral vein and inferior vena cavography were performed,and aspiration thrombus technique and thrombolysis of intracavitary were used to eliminate thrombus.After angiography of IVC,the recanalization of IVC was performed,which was followed by thrombus aspiration technique and thrombolysis of intracavitary.Finally,IVC was dilated with percutaneous transluminal balloon angioplasty.The clinical observation was used by color Doppler post-discharge. Results:All the patients occlusion was barrier diaphragm before operation,adopting this treatment,7 patients thrombus was eliminated instantly,after using intraductal thrombolysis,2 patients thrombus was eliminated 4 days later,2 patients thrombus was eliminated 7 days later.And pulmonary embolism was not happened with percutaneous transluminal balloon angioplasty.In following-up visit,blood current of IVC was continuous,there had no re-occlusion or thrombus happening. Conclusions:The therapeutic efficacy of thrombus aspiration technique and thrombolysis of intracavitary and PTA for BCS complicated with IVC thrombus is safe and effective.
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Key words:
- gastric neoplasms /
- telomerase /
- matrix metalloproteinase-7 /
- expression
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