游泳运动对高脂诱导肥胖SD大鼠Ghrelin表达的影响
Effects of swimming exercise on Ghrelin levels in high-diet induced obesity SD rats
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摘要: 目的：观察游泳运动干预后肥胖大鼠血清及下丘脑Ghrelin水平的变化，探讨其在肥胖及代谢紊乱中的作用。方法：SD大鼠50只随机分为普食(NC，n=10)组和高脂(HD，n=40)组。筛选出成功建立肥胖模型20只随机分为肥胖对照(OB)组、游泳(SWI)组，各10只。SWI组予以每天1 h，每周5 d，共行6周游泳训练；OB组及NC组予等剂量蒸馏水灌胃。分别测定干预6周前后各组体重(BW)、空腹血糖(FPG)、三酰甘油(TG)、总胆固醇(TCH)、空腹胰岛素(FINS)、胰岛素抵抗指数(HOMA-IR)及血清Ghrelin。免疫组织化学法检测各组大鼠下丘脑Ghrelin表达水平。结果：干预前，OB组、SWI组均较NC组BW、FPG、TG、TCH、FINS、HOMA-IR显著升高，Ghrelin明显降低(P0．01)；干预后，SWI组均较OB组BW、TG、TCH、FPG、FINS、HOMA-IR明显降低，Ghrelin水平升高(P0．01)。免疫组织化学显示OB组下丘脑Ghrelin表达较NC组降低(P0．01)，SWI组较OB组升高(P0．05)。结论：游泳运动可改善胰岛素敏感性并减轻体重，降低肥胖大鼠Ghrelin水平。Abstract: Objective:To observe the changes of Ghrelin level in serum and hypothalamus of high-diet induced obesity rats after 6-week swimming exercise, and to investigate the association of Ghrelin with obesity and metabolic syndrome. Methods:Fifty SD rats were randomly divided into normal diet group(NC, n=10) and high fat diet group(HD, n=40). Obesity models(n=20) were established from the HD group,and equally assigned to obesity group(OB) and swimming exercise group(SWI). The SWI group received standard swimming exercise 1 hour/day,5 day/week for 6 weeks,and the other two groups were given distilled water. Their body weight(BW), fasting plasma glucose(FPG),triglyceride(TG),total cholesterol(TCH),fasting insulin(FINS),HOMA index and fasting Ghrelin were measured 6 weeks before and after the intervene; the Ghrelin expression in the hypothalamus was detected by immunohistochemistry. Results:The BW,FPG,TG,TCH,FINS levels and HOMA-IR in the OB group and in the SWI group were increased and the Ghrelin level was decreased in contrast to the NC group before the intervene(P0.01);the BW,FPG,TG,TCH,FINS levels and HOMA-IR in the SWI group were decreased and the Ghrelin level was increased compared to that in the OB group(P0. 01) after the intervene. Immunohistochemistry results showed that the Ghrelin expression level was increased in the hypothalamus of the OB group but was down-regulated in the SWI group(P0. 05). Conclusions:Swimming exercise can improve the insulin resistance and reduce the weight and Ghrelin levels in obesity rats.
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Key words:
- obesity /
- models,animal /
- swimming /
- Ghrelin /
- rat
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