人乳头状瘤病毒和EB病毒在子宫颈上皮内瘤变中的表达

钟辉; 刘锦钰; 龙璐雯

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人乳头状瘤病毒和EB病毒在子宫颈上皮内瘤变中的表达

钟辉 , 刘锦钰 , 龙璐雯

文章导航 > 蚌埠医学院学报 > 2012, 36 > 7: (765-768)

引用本文:

Citation:

人乳头状瘤病毒和EB病毒在子宫颈上皮内瘤变中的表达

1.
1. 安徽省淮北市妇幼保健院病理科;
2.
2. 安徽省淮北市妇幼保健院妇科 235000

作者简介: 钟辉(1974-),男,主治医师．

基金项目:
安徽省淮北市科技局基金资助项目(201002043)

Expression of human papillomavirus and Epstein-Barr virus in cervical intraepithelial neoplasia

1.
1. Department of Pathology;
2.
2. Department of Gynecology, Huaibei Maternal and Child Hospital, Huaibei Anhui 235000, China

摘要: 目的:探讨人乳头状瘤病毒(HPV)和EB病毒(EBV)在子宫颈上皮内瘤变(CIN)中的表达。方法:采用原位杂交对130例CIN(CIN1 50例,CIN2 40例,CIN3 40例)、子宫颈癌10例、子宫颈湿疣样病变10例、子宫颈正常黏膜10例的HPV(高危型16/18、低危型6/11)和EBV进行检测。结果:HPV16/18在CIN1、CIN2、CIN3的阳性表达率分别是44.0%、37.5%和62.5%,子宫颈癌为8/10,子宫颈湿疣样病变为10/10,子宫颈正常黏膜为3/10,差异有统计学意义(P0.01)。HPV6/11在CIN1、CIN2、CIN3的表达率分别是36.0%、52.5%和70.0%,在子宫颈癌中的表达是6/10,子宫颈湿疣样病变9/10,子宫颈正常黏膜3/10,差异有统计学意义(P0.01)。HPV16/18和HPV6/11在CIN1、CIN2、CIN3中联合表达率分别为26.0%、22.5%和50.0%,子宫颈癌为6/10,子宫颈湿疣样病变为7/10,子宫颈正常黏膜为2/10,差异有统计学意义(P0.01)。EBV在CIN1、CIN2、CIN3的阳性表达率分别是24.0%、12.5%和22.5%,子宫颈癌为3/10,子宫颈湿疣样病变为0/10,子宫颈正常黏膜为0/10,差异无统计学意义(P0.05)。结论:HPV感染是引起CIN的主要致病因子,临床上往往是多种亚型混合感染。EB病毒在CIN和子宫颈癌表达的意义仍有争议。
- 子宫颈肿瘤 /
- 子宫颈上皮内瘤变 /
- 人乳头状瘤病毒 /
- EB病毒 /
- 病理学,临床
Abstract: Objective:To explore the expression of human papillomavirus(HPV) and Epstein-Barr virus(EBV) in cervical intraepithelial neoplasia(CIN).Methods:The high-risk HPV(16/18),low-risk HPV(6/11)and EBV of 130 cases of CIN(50 CIN1,40 CIN2,40 CIN3),10 cases of invasive cervical carcinomas,10 cervical condyloma-like lesions and 10 normal mucosa of the uterine cervix were detected using in situ hybridization(ISH) technique.Results:The positive expression rates of HPV16/18 in CIN1,CIN2 and CIN3 were 44.0%,37.5% and 62.5%,respectively;and positive HPV16/18 was detected in 8/10 of the invasive cervical carcinomas,10/10 of cervical condyloma-like lesions and 3/10 of normal mucosa of the uterine cervix(P0.01).The positive expression rates of HPV6/11 in CIN1,CIN2 and CIN3 were 36.0%,52.5% and 70.0%,respectively;and positive HPV6/11 was detected in 6/10 of the invasive cervical carcinomas,9/10 of cervical condyloma-like lesions and 3/10 of normal mucosa of the uterine cervix(P0.01).The combined positive expression rates of HPV16/18 and HPV6/11 in CIN1,CIN2 and CIN3 were 26.0%,22.5% and 50.0%,respectively;and combined positive expression of HPV16/18 and HPV6/11 was detected in 6/10 of the invasive cervical carcinomas,7/10 of cervical condyloma-like lesions and 2/10 of normal mucosa of the uterine cervix(P0.01).The positive expression rates of EBV in CIN1,CIN2 and CIN3 were 24.0%,12.5% and 22.5%,respectively;and EBV was detected positive in 3/10 of the invasive cervical carcinomas,0/10 of cervical condyloma-like lesions and 0/10 of normal mucosa of the uterine cervix(P0.05).Conclusions:HPV infection is the major etiological factor for CIN,clinically often coinfected with some variants.The signification of positive expression of EBV in CINs and invasive cervical carcinomas is still debatable.
- cervix neoplasms /
- cervical intraepithelial neoplasia /
- human papillomavirus /
- Epstein-Barr virus /
- pathology,clinical

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人乳头状瘤病毒和EB病毒在子宫颈上皮内瘤变中的表达

作者简介: 钟辉(1974-),男,主治医师．

1. 1. 安徽省淮北市妇幼保健院病理科;
2. 2. 安徽省淮北市妇幼保健院妇科 235000

收稿日期: 2012-02-02
网络出版日期: 2012-07-15

基金项目: 安徽省淮北市科技局基金资助项目(201002043)

关键词:

摘要: 目的:探讨人乳头状瘤病毒(HPV)和EB病毒(EBV)在子宫颈上皮内瘤变(CIN)中的表达。方法:采用原位杂交对130例CIN(CIN1 50例,CIN2 40例,CIN3 40例)、子宫颈癌10例、子宫颈湿疣样病变10例、子宫颈正常黏膜10例的HPV(高危型16/18、低危型6/11)和EBV进行检测。结果:HPV16/18在CIN1、CIN2、CIN3的阳性表达率分别是44.0%、37.5%和62.5%,子宫颈癌为8/10,子宫颈湿疣样病变为10/10,子宫颈正常黏膜为3/10,差异有统计学意义(P0.01)。HPV6/11在CIN1、CIN2、CIN3的表达率分别是36.0%、52.5%和70.0%,在子宫颈癌中的表达是6/10,子宫颈湿疣样病变9/10,子宫颈正常黏膜3/10,差异有统计学意义(P0.01)。HPV16/18和HPV6/11在CIN1、CIN2、CIN3中联合表达率分别为26.0%、22.5%和50.0%,子宫颈癌为6/10,子宫颈湿疣样病变为7/10,子宫颈正常黏膜为2/10,差异有统计学意义(P0.01)。EBV在CIN1、CIN2、CIN3的阳性表达率分别是24.0%、12.5%和22.5%,子宫颈癌为3/10,子宫颈湿疣样病变为0/10,子宫颈正常黏膜为0/10,差异无统计学意义(P0.05)。结论:HPV感染是引起CIN的主要致病因子,临床上往往是多种亚型混合感染。EB病毒在CIN和子宫颈癌表达的意义仍有争议。

English Abstract

Expression of human papillomavirus and Epstein-Barr virus in cervical intraepithelial neoplasia

1. 1. Department of Pathology;
2. 2. Department of Gynecology, Huaibei Maternal and Child Hospital, Huaibei Anhui 235000, China

Received Date: 2012-02-02
Available Online: 2012-07-15

Abstract: Objective:To explore the expression of human papillomavirus(HPV) and Epstein-Barr virus(EBV) in cervical intraepithelial neoplasia(CIN).Methods:The high-risk HPV(16/18),low-risk HPV(6/11)and EBV of 130 cases of CIN(50 CIN1,40 CIN2,40 CIN3),10 cases of invasive cervical carcinomas,10 cervical condyloma-like lesions and 10 normal mucosa of the uterine cervix were detected using in situ hybridization(ISH) technique.Results:The positive expression rates of HPV16/18 in CIN1,CIN2 and CIN3 were 44.0%,37.5% and 62.5%,respectively;and positive HPV16/18 was detected in 8/10 of the invasive cervical carcinomas,10/10 of cervical condyloma-like lesions and 3/10 of normal mucosa of the uterine cervix(P0.01).The positive expression rates of HPV6/11 in CIN1,CIN2 and CIN3 were 36.0%,52.5% and 70.0%,respectively;and positive HPV6/11 was detected in 6/10 of the invasive cervical carcinomas,9/10 of cervical condyloma-like lesions and 3/10 of normal mucosa of the uterine cervix(P0.01).The combined positive expression rates of HPV16/18 and HPV6/11 in CIN1,CIN2 and CIN3 were 26.0%,22.5% and 50.0%,respectively;and combined positive expression of HPV16/18 and HPV6/11 was detected in 6/10 of the invasive cervical carcinomas,7/10 of cervical condyloma-like lesions and 2/10 of normal mucosa of the uterine cervix(P0.01).The positive expression rates of EBV in CIN1,CIN2 and CIN3 were 24.0%,12.5% and 22.5%,respectively;and EBV was detected positive in 3/10 of the invasive cervical carcinomas,0/10 of cervical condyloma-like lesions and 0/10 of normal mucosa of the uterine cervix(P0.05).Conclusions:HPV infection is the major etiological factor for CIN,clinically often coinfected with some variants.The signification of positive expression of EBV in CINs and invasive cervical carcinomas is still debatable.