溃疡性结肠炎大鼠免疫功能的变化

王爱磊; 张学明

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溃疡性结肠炎大鼠免疫功能的变化

王爱磊 , 张学明

文章导航 > 蚌埠医学院学报 > 2013, 37 > 6: (661-662,665)

引用本文:

Citation:

溃疡性结肠炎大鼠免疫功能的变化

王爱磊¹,
张学明²

1.
1. 河北省唐山市中医医院肛肠科;
2.
2. 河北省唐山市工人医院肛肠科

作者简介: 王爱磊(1975－)，男，副主任医师．

基金项目:

Experimental study on the immune function of ulcerative colitis

WANG Ai-lei¹,
ZHANG Xue-ming²

1.
1. Department of Anorectal Surgery, Tangshan Traditional Chinese Medicine Hospital, Tangshan Hebei 063000;
2.
2. Department of Anorectal Surgery, Tangshan Gongren Hospital, Tangshan Hebei 063000, China

摘要: 目的：研究三硝基苯磺酸(TNBS)/乙醇致溃疡性结肠炎(UC)大鼠免疫球蛋白、补体及红细胞免疫功能的变化。方法：48只大鼠随机分为对照组、模型1组、模型2组、模型3组，每组12只。模型组用0.5ml含20mgTNBS的30%乙醇灌肠制备UC大鼠模型，对照组用0.9%氯化钠注射液0.5ml灌肠。检测UC大鼠血清免疫球蛋白、补体及红细胞免疫功能的变化。结果：造模后1周、3周、7周模型组大鼠外周血IgA、造模1周和3周IgG和红细胞免疫复合物含量均明显高于对照组(P0.01)，在造模1周时表达最高，3周时已有下降，7周时与1周模型差异均有统计学意义(P0.01)；IgM与对照组差异无统计学意义(P0.05)。而补体C3和红细胞表面C3b受体在3个时间段及造模后1周与3周C4均较对照组降低(P0.01)。结论：免疫球蛋白、补体及红细胞免疫功能的变化，说明UC大鼠存在免疫功能亢进，导致循环免疫复合物在肠黏膜沉积，进而引发肠黏膜局部变态反应。
- 结肠炎，溃疡性 /
- 免疫学 /
- 大鼠
Abstract: Objective:To investigate the variation of the immune functions in ulcerative colitis(UC)rats,including the immune globulin,complement and red blood cell(rBC).Methods:Forty-eight rats were randomly divided into control group model group 1,model group 2 and model group 3,with 12 rats in each.The model groups were given 0.5 ml 30% alcohol enema containing 20 mgtrinitrobenzene-sulfonic acid(TNBS)to construct UC rat models and the control group was given 0.5 ml physiological saline enema.The changes of the immune globulin complement and rBC immune function were detected.Results:The content of IgA and rBC circulating immune complexes in the blood serum of the model group increased predominantly at the first,third and seventh week as compared with those of the normal group(P0.01);the content of IgG in the model group increased predominantly at the first and third week as compared with that in the normal group(P0.01).The expressions of IgA,rBC circulating immune complexes and IgG were the highest in the model group at the first week,which began to decrease at the third one,and the expression at the seventh and the first week was statistically different(P0.01).IgM had no significant deviation between the model groups and the control group(P0.05),but C3 and red blood cell surface C3b receptor in the model group were lower than those in the control group at the first,third and seventh week(P0.01).The content of C4 in the model group was lower at the first and the third week than that in the control group(P0.01).Conclusions:The changes of immune globulin,complement and rBC immune function reveal that abnormal increasing of immunoresponse may lead to deposition of circulating immune complexes in the intestinal mucosa of the UC rats and activate local allergy.
- colitis,ulcerative /
- immunology /
- rats

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溃疡性结肠炎大鼠免疫功能的变化

王爱磊¹
张学明²

作者简介: 王爱磊(1975－)，男，副主任医师．

1. 1. 河北省唐山市中医医院肛肠科;
2. 2. 河北省唐山市工人医院肛肠科

收稿日期: 2012-07-06
网络出版日期: 2013-06-15

基金项目:

关键词:

摘要: 目的：研究三硝基苯磺酸(TNBS)/乙醇致溃疡性结肠炎(UC)大鼠免疫球蛋白、补体及红细胞免疫功能的变化。方法：48只大鼠随机分为对照组、模型1组、模型2组、模型3组，每组12只。模型组用0.5ml含20mgTNBS的30%乙醇灌肠制备UC大鼠模型，对照组用0.9%氯化钠注射液0.5ml灌肠。检测UC大鼠血清免疫球蛋白、补体及红细胞免疫功能的变化。结果：造模后1周、3周、7周模型组大鼠外周血IgA、造模1周和3周IgG和红细胞免疫复合物含量均明显高于对照组(P0.01)，在造模1周时表达最高，3周时已有下降，7周时与1周模型差异均有统计学意义(P0.01)；IgM与对照组差异无统计学意义(P0.05)。而补体C3和红细胞表面C3b受体在3个时间段及造模后1周与3周C4均较对照组降低(P0.01)。结论：免疫球蛋白、补体及红细胞免疫功能的变化，说明UC大鼠存在免疫功能亢进，导致循环免疫复合物在肠黏膜沉积，进而引发肠黏膜局部变态反应。

English Abstract

Experimental study on the immune function of ulcerative colitis

WANG Ai-lei¹
ZHANG Xue-ming²

1. 1. Department of Anorectal Surgery, Tangshan Traditional Chinese Medicine Hospital, Tangshan Hebei 063000;
2. 2. Department of Anorectal Surgery, Tangshan Gongren Hospital, Tangshan Hebei 063000, China

Received Date: 2012-07-06
Available Online: 2013-06-15

Abstract: Objective:To investigate the variation of the immune functions in ulcerative colitis(UC)rats,including the immune globulin,complement and red blood cell(rBC).Methods:Forty-eight rats were randomly divided into control group model group 1,model group 2 and model group 3,with 12 rats in each.The model groups were given 0.5 ml 30% alcohol enema containing 20 mgtrinitrobenzene-sulfonic acid(TNBS)to construct UC rat models and the control group was given 0.5 ml physiological saline enema.The changes of the immune globulin complement and rBC immune function were detected.Results:The content of IgA and rBC circulating immune complexes in the blood serum of the model group increased predominantly at the first,third and seventh week as compared with those of the normal group(P0.01);the content of IgG in the model group increased predominantly at the first and third week as compared with that in the normal group(P0.01).The expressions of IgA,rBC circulating immune complexes and IgG were the highest in the model group at the first week,which began to decrease at the third one,and the expression at the seventh and the first week was statistically different(P0.01).IgM had no significant deviation between the model groups and the control group(P0.05),but C3 and red blood cell surface C3b receptor in the model group were lower than those in the control group at the first,third and seventh week(P0.01).The content of C4 in the model group was lower at the first and the third week than that in the control group(P0.01).Conclusions:The changes of immune globulin,complement and rBC immune function reveal that abnormal increasing of immunoresponse may lead to deposition of circulating immune complexes in the intestinal mucosa of the UC rats and activate local allergy.