Fas与FasL在兔视网膜缺血再灌注损伤中的表达及rh-bFGF对其表达的影响
The expressions of Fas and FasL in rabbit retina with ischemia-reperfusion injury, and the effect of recombinant human basic fibroblast growth factor on its expression
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摘要: 目的:探讨重组人碱性成纤维细胞生长因子(rh-bFGF)对兔视网膜缺血再灌注损伤(RIRI)的保护作用。方法:应用前房加压灌注法,升高眼内压,造兔视网膜RIRI模型。外眼及眼底正常纯种大耳白兔66只,随机分为正常假手术组(6只)、RIRI组(30只)和RIRI+rh-bFGF组(30只),均选取右眼为实验眼。RIRI组和RIRI+rh-bFGF组在建立RIRI模型后,前者给予0.9%氯化钠注射液,后者给予rh-bFGF药物治疗。常规HE染色观察视网膜组织细胞形态学的变化,免疫组织化学法检测RIRI组和RIRI+rh-bFGF组6、12、24、48、72 h 5个时间点视网膜组织中Fas、FasL蛋白的表达变化。结果:正常假手术组兔视网膜未见凋亡细胞;RIRI组视网膜的内核层和神经节细胞层可见凋亡细胞,且细胞凋亡在24 h达到高峰。Fas在正常假手术组视网膜组织中未见明显表达;其他2组RIRI后6 h开始有少量表达,至24 h时达到高峰,随后48 h开始下降,到72 h后明显减弱。FasL在正常假手术组视网膜组织中未见明显表达;其他2组RIRI后12 h表达开始升高,至24 h达到高峰,之后略有下降,至72 h依旧可见较高水平表达。RIRI+rh-bFGF组与RIRI组相比较,各时间点凋亡因子阳性表达趋势大致相同,且Fas、FasL表达均明显减弱(P<0.01),2组在6、12、24、48 h各时间点表达差异均有统计学意义(P<0.01)。结论:rh-bFGF对兔RIRI保护作用机制可能与抑制视网膜内Fas、FasL表达有关。
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关键词:
- 再灌注损伤 /
- 视网膜 /
- 重组人碱性成纤维细胞生长因子 /
- 兔
Abstract: Objective:To investigate the protective effects of recombinant human basic fibroblast growth factor(rh-bFGF) on retinal ischemia-reperfusion injury(RIRI) in rabbit.Methods:The models of RIRI in rabbit were established by elevating intraocular pressure.Sixty-six healthy rabbits with normal external ocular and fundus were randomly divided into the sham-operation group(6 rabbits), retinal ischemia-reperfusion injury group(RIRI group, 30 rabbits) and rh-bFGF treatment group(RIRI+rh-bFGF group, 30 rabbits).The right eye was selected as the experimental eye.RIRI group and RIRI+rh-bFGF group were treated with 0.9% sodium chloride injection and rh-bFGF, respectively.Morphological changes of retinal cells and the expressions of Fas and FasL of retinal tissue in sham-operation group, and at 6 h, 12 h, 24 h, 48 h and 72 h time-points of RIRI and RIRI+rh-bFGF groups were detected by HE staining and immunohistochemical method, respectively.Results:No apoptotic cells was found in sham-operation group.Apoptotic cells in RIRI group located mainly in the inner nuclear layer and the ganglion cell layer, and which arrived at peak at 24 h after reperfusion.No obvious expression of Fas in retinal tissue of the sham-operation group was found.After RIRI, little Fas expressing at 6 h, arriving at peak at 24 h, then begaining to decrease at 48 h and significant weakening at 72 h were found.No obvious expression of FasL in retinal tissue of the sham-operation group was found.After RIRI, FasL expression beginning to increase at 12 h, arriving at peak at 24 h, then beginning to decrease and still maintaining a high level at 72 h were found.Compared with the RIRI group, the positive expression trend of apoptotic factors at each time-point in RIRI+rh-bFGF group was similar, the expressions of Fas and FasL in RIRI group and RIRI+rh-bFGF group obviously decreased(P<0.05).The differences of the expression of Fas and FasL at 6 h, 12 h, 24 h, 48 h and 72 h between RIRI and RIRI+rh-bFGF group were statistical significance(P<0.01).Conclusions:The protective mechanism of rh-bFGF on RIRI in rabbit may be related to inhibit the expressions of Fas and FasL in retina.-
Key words:
- reperfusion injury /
- retina /
- recombinant human basic fibroblast growth factor /
- rabbit
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[1] WANG XD,KASHⅡ S,ZHAO L,et al. Vitam in B6 protects primate retinal neurons from ischemic injury[J]. Brain Res, 2002,940(1/2):36. [2] 赵成,游志鹏. 视网膜缺血再灌注损伤的治疗进展[J]. 国际眼科杂志,2007,7(2):475. [3] KMI HS,PARK CK. Retinal ganglion cell death is delayed by activation of retinal intrinsic cell survival program[J]. Brain Res, 2005,1057(1/2):17. [4] 曾环想,张宏波,黄凯,等. 碱性成纤维细胞生长因子的药学研究进展[J]. 中国新药杂志,2010,11(19):949. [5] 王小兵,王晓健,张宝林. 不同浓度的重组人碱性成纤维细胞生长因子对离体人表皮细胞增殖的影响[J]. 中国药物与临床,2008,8(12):969. [6] FOULDS WS, JOHNSON NF. Rabbit electroretinogram during recovery from induced ischaemia[J]. Trans Ophthalmol Soc UK, 1974,94(2):383. [7] 刘朋朋,张琦. 外源性H2S预处理对大鼠RIRI细胞凋亡的影响[J]. 国际眼科杂志,2012,12(1):33. [8] MIYAKI K,MATSUBARA A,NISHIWAKI A,et al. Pitavastatin attenuates leukocyte-endothelial interactions induced by ischemia-reperfusion injury in the rat retina[J]. Curr Eye Res,2009,34(1):10. [9] NAGATA S,GOLSTEIN P. The Fas death factor[J]. Science, 1995,267(5203):1449. [10] GRIFFITH TS,BRUNNER T,FLETCHER SM,et al. Fas ligand-induced apoptosis as a mechanism of immune privilege[J]. Science,1995,270(5239):1189. [11] 刘索新,鞠学红. 神经营养因子对视网膜缺血再灌注损伤的影响[J]. 解剖科学进展,2013,19(5):454. [12] 孟根托娅,闫元奎,赵海霞. 实验性视网膜脱离时外源性bFGF对视网膜细胞凋亡的干预作用[J]. 中国实用眼科杂志,2009,27(9):1055. [13] 王彩荣,王超英. 碱性成纤维细胞生长因子在眼科的研究与应用[J]. 临床误诊误治,2009,22(5):73. -
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