血清UCH-L1蛋白在新生大鼠缺氧缺血性脑病评估中的价值
Application value of the serum ubiquitin carboxyl-terminal esterase L1 protein level in the diagnosis of hypoxic-ischemic encephalopathy in neonatal rats
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摘要: 目的：探讨血清泛素羧基末端水解酶-1（UCH-L1）蛋白在新生大鼠缺氧缺血性脑病中的价值。方法：将168只新生大鼠随机分为对照组、手术组及缺氧缺血性脑损伤（HIBD）组，每组56只；各组组内又随机分为7个亚组：6 h组、12 h组、24 h组、48 h组、72 h组、96 h组及7 d组，每亚组8只；HIBD组采用改良Rice法制备HIBD模型。各组大鼠于不同时间点处死取血，采用ELISA法检测血清中UCH-L1表达水平的变化。结果：不同时间点3组大鼠间UCH-L1蛋白表达水平差异均有统计学意义（P<0.01），HIBD组UCH-L1蛋白表达水平显著高于对照组和手术组（P<0.01），而对照组与手术组间UCH-L1蛋白表达水平差异无统计学意义（P>0.05）。HIBD组大鼠各时间点UCH-L1蛋白表达水平差异有统计学意义（P<0.01）；HIBD组血清UCH-L1蛋白在6 h时检测水平明显升高，48 h到达峰值，后逐渐下降，且脑组织损伤越严重，血清UCH-L1蛋白表达水平越高。结论：UCH-L1蛋白血清表达水平与脑组织损伤的严重程度表现一致，可作为检测指标应用于评估脑组织损伤的严重程度。
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关键词:
- 脑缺血 /
- 脑缺氧 /
- 血清泛素羧基末端水解酶-1蛋白 /
- 大鼠
Abstract: Objective:To explore the application value of serum level of ubiquitin carboxyl-terminal esterase L1(UCH-L1) in the diagnosis of hypoxic-ischemic encephalopathy in newborn rats.Methods:One hundred and sixty eighty newborn SD rats were randomly divided into the control group,sham-operation group and hypoxic-ischemic brain damage(HIBD) model group(56 rats each group).Each group was randomly subdivided into the 6 h,12 h,24 h,48 h,72 h,96 h and 7 d groups(8 rats each subgroup).The HIBD model was established using the improved Rice method.All rats were sacrificed to obtain blood at different time points.The serum levels of UCH-L1 were tested by ELISA.Results:At different time-points,the differences of the levels of UCH-L1 between three groups were statistically significant(P<0.01),the levels of UCH-L1 in HIBD model group were significantly higher than that in control group and sham-operation group(P<0.01),and the difference between the control group and sham-operation group was not statistically significant(P>0.05).The differences of the levels of UCH-L1 in HIBD model group between different time-points were statistically significant(P<0.01).In HIBD model group,the serum levels of UCH-L1 began to significantly increase at 6 h,reached the peak value at 48 h,then decreased gradually.The more severe the brain damaged,the higher the level of serum UCH-L1 was.Conclusions:The levels of UCH-L1 is positively correlated with the severity of the brain damage.The expression of UCH-L1 can be used an indicator in evaluating the brain damage. -
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