住院儿童EB病毒感染临床分析
Clinical analysis of the Epstein-Barr virus infection in hospitalized children
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摘要: 目的：了解云南省昆明市住院患儿EB病毒（EBV）感染状况及相关疾病谱临床流行分布特征。方法：回顾性统计分析疑似EBV感染住院患儿经PCR荧光法检测全血中EBV核酸阳性病例的临床资料。结果：共送检住院患儿血样本547例，EBV核酸检测阳性例数97例，检出率17.73%，其中男56例（57.73%），女41例（42.27%）；平均年龄（4.60±2.84）岁，学龄前儿童（0~6岁）76例，占检出人数的78.35%。儿童EBV感染临床症状多样，常以发热、咳嗽、淋巴结肿大等上呼吸道感染症状首诊，多以EB病毒感染、肺炎、支气管炎、传染性单核细胞增多症、咽炎等临床表现为主，可伴有肝-脾等肿大、皮疹、肝脏和心肌损害等并发症状；同时检出合并其他病原体感染71例次，其中肺炎支原体19例（19.59%），伤寒或副伤寒杆菌18例（18.56%），乙型脑炎病毒17例（17.53%），副流感病毒8例（8.25%），肠道病毒2例（2.06%），其他病毒共7例（7.22%），合并感染患儿47例（42.27%）；且各季节时段的检出率差异无统计学意义（P>0.05）。结论：EBV感染是昆明地区住院患儿呼吸道感染的重要病原体之一，各季节时段均可发病，常呈现与其他病原体混合感染。Abstract: Objective:To investigate the Epstein-Barr virus(EBV) infection condition in hospitalized children from kunming city of Yunnan province,and related clinical popular distribution characteristics of disease spectrum.Methods:The clinical data of children suspected with EBV infection,the EBV nucleic acid positive cases identified using PCR fluorescence method,were retrospectively analyzed.Results:Among 547 blood samples,97 cases with positive EBV nucleic acid were detected,the detection rate of which was 17.73%,which included 56 males(57.73%) and 41 females(42.27%).The mean age of all cases was(4.60±2.84) years,and the pre-school children(0 to 6 years old) in 76 cases(78.35%) were found.The clinical symptoms of children infected with EBV was various.The upper respiratory tract infection symptoms(including fever,cough and lymph node enlargement) were the first symptoms of the EB V infection,and the pneumonia,bronchitis,infectious mononucleosis and pharyngitis were the clinical manifestations,which was accompanied by liver and spleen enlargement,rash,liver and heart muscle function damage.The combined pathogen infection in 71 cases were identified,which included mycoplasma pneumoniae infection in 19 cases(19.59%),typhoid and paratyphoid infection in 18 cases(18.56%),Japanese encephalitis virus infection in 17 cases(17.53%),parainfluenza infection in 8 cases(8.25%),intestinal virus infection in 2 cases(2.06%),other viruses in 7 cases(7.22%) and co-infection in 47 cases(42.27%).The difference of the detection rate in different seasons was not statstically significant(P>0.05).Conclusions:EBV infection is one of the most important pathogen of respiratory tract infection in hospitalized children in Kunming region,the disaese can occure in each season,and the mixture infection with other pathogen is common.
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Key words:
- Epstein-Barr virus /
- DNA detection /
- epidemiology /
- child
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